Recombinant Staphylococcus epidermidis Lantibiotic epidermin (epiA)

中文名稱：

表皮葡萄球菌epiA重組蛋白
品名簡稱：

Recombinant Staphylococcus epidermidis epiA protein
貨號：

CSB-EP357305FLL
說明書：

MSDS Datasheet
規格：

￥2328
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

epiA
Uniprot No.：

P08136
別名：

epiALantibiotic epidermin
種屬：

Staphylococcus epidermidis
蛋白長度：

Full Length of Mature Protein
來源：

E.coli
分子量：

17.7 kDa
表達區域：

31-52aa
氨基酸序列

IASKFICTPGCAKTGSFNSYCC
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-KSI-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Tris-based buffer，50% glycerol
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產品描述：

In the production of recombinant S. epidermidis epiA protein, the gene for epiA (E.coli) was cloned into a vector and expressed as epiA protein in E.coli. The plasmids with the copy of epiA, or the expression vector, were often used to enhance gene expression. Every step of production was undergone with a strict QC system. N-terminal 6xHis-KSI tag was used in the process. The purity is 85% determined by SDS-PAGE.
The Staphylococcus epidermidis genes encoding the His-tag-labelled epidermin precursor peptide EpiA. The 52-amino acid prepeptide EpiA consists of an N-terminal leader peptide with amino acids - 30 to - 1 and a C-terminal pro-epidermin with amino acids + 1 to + 22. EpiA is post-translationally converted to the mature antibiotic by dehydration of serine and threonine residues, sulfide bridge formation, oxidative decarboxylation at the C-terminus and removal of the leader peptide. enzymes involved in epidermin biosynthesis. Studies identified that EpiA was expressed in Escherichia coli using a malE-epiA fusion. The identity of purified EpiA was confirmed by ion spray mass spectrometry and amino acid sequencing. For EpiA detection, anti-EpiA antisera were raised. Upon prolonged incubation, factor Xa not only cleaved EpiA from the fusion protein, but also less efficiently cleaved EpiA internally between R-1 and I + 1.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Lanthionine-containing peptide antibiotic (lantibiotic) active on Gram-positive bacteria. The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores.
蛋白家族：

Type A lantibiotic family