Recombinant Staphylococcus aureus Peptide deformylase (def)

^{In
Stock}

中文名稱：

Recombinant Staphylococcus aureus Peptide deformylase(def)
貨號：

CSB-YP017707FKZ
說明書：

MSDS Datasheet
規格：

￥2616
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
問答及客戶評論
靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

def
Uniprot No.：

P68826
別名：

def; def1; pdf1Peptide deformylase; PDF; EC 3.5.1.88; Polypeptide deformylase
種屬：

Staphylococcus aureus
蛋白長度：

Full Length
來源：

Yeast
分子量：

22.6kDa
表達區域：

1-183aa
氨基酸序列

MLTMKDIIRDGHPTLRQKAAELELPLTKEEKETLIAMREFLVNSQDEEIAKRYGLRSGVGLAAPQINISKRMIAVLIPDDGSGKSYDYMLVNPKIVSHSVQEAYLPTGEGCLSVDDNVAGLVHRHNRITIKAKDIEGNDIQLRLKGYPAIVFQHEIDHLNGVMFYDHIDKNHPLQPHTDAVEV
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

問答及客戶評論

■ 常見問題解答

I have purchased your E. coli expressed def recombinant protein (cat#: CSB-EP017707FKZ; Lot#: 03380).I would like to develop a stable recombinant peptide deformylase. The half-life of this enzyme is really short (10 seconds) and I want to improve it.Do you have any information on how to increase the half-life?

Very nice to receive your inquiry. We're sorry but we haven't performed related experiment to study the half-life of this protein and haven't received any relevant feedback from other customers.
You has mentioned that "The half-life of this enzyme is really short (10 seconds)". What analysis did you do? How did you get this conclusion?
Here we provide a literature for your reference. Here is the link: http://europepmc.org/articles/PMC2312423

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靶點詳情

功能：

Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions.
蛋白家族：

Polypeptide deformylase family