Recombinant Salmonella typhimurium DNA gyrase subunit B (gyrB), partial

中文名稱：

鼠傷寒沙門菌gyrB重組蛋白
貨號：

CSB-YP363256SXB
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

鼠傷寒沙門菌gyrB重組蛋白
貨號：

CSB-EP363256SXB
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

鼠傷寒沙門菌gyrB重組蛋白
貨號：

CSB-EP363256SXB-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

鼠傷寒沙門菌gyrB重組蛋白
貨號：

CSB-BP363256SXB
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

鼠傷寒沙門菌gyrB重組蛋白
貨號：

CSB-MP363256SXB
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

gyrB
Uniprot No.：

P0A2I3
別名：

gyrB; STM3835; DNA gyrase subunit B; EC 5.6.2.2
種屬：

Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state, and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings. Replenishes negative supercoiling downstream of highly transcribed genes to help control overall chromosomal supercoiling density. E.coli makes 15% more negative supercoils in pBR322 plasmid DNA than S.typhimurium; the S.typhimurium GyrB subunit is toxic in E.coli, while the E.coli copy can be expressed in S.typhimurium even though the 2 subunits have 777/804 residues identical.; Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.
基因功能參考文獻：
1. GyrB652 mutant is not inactive at 42 degrees C in vivo or in vitro and doesn't directly produce breaks in chromosomal DNA; it has a low k(cat) compared to wild-type GyrB subunit PMID: 16267301
亞細胞定位：

Cytoplasm.
蛋白家族：

Type II topoisomerase GyrB family
數據庫鏈接：

KEGG: stm:STM3835

STRING: 99287.STM3835