Recombinant Saccharomyces cerevisiae Mediator of RNA polymerase II transcription subunit 8 (MED8)

中文名稱：

釀酒酵母MED8重組蛋白
貨號：

CSB-YP013668SVG
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

釀酒酵母MED8重組蛋白
貨號：

CSB-EP013668SVG
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

釀酒酵母MED8重組蛋白
貨號：

CSB-EP013668SVG-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

釀酒酵母MED8重組蛋白
貨號：

CSB-BP013668SVG
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

釀酒酵母MED8重組蛋白
貨號：

CSB-MP013668SVG
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

MED8
Uniprot No.：

P38304
別名：

MED8; YBR193C; YBR1403Mediator of RNA polymerase II transcription subunit 8; Mediator complex subunit 8
種屬：

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
蛋白長度：

Full Length of Mature Protein
表達區域：

2-223
氨基酸序列

SQSTASLVP EGNQGSLQED VSFDFNGVPG QALDAVRMRL AQLTHSLRRI RDEMSKAELP QWYTLQSQLN VTLSQLVSVT STLQHFQETL DSTVVYPLPK FPTTSHESLV TTLLRKKNIP EVDEWMKYVR ETSGVTTALL KDEEIEKLLQ QDREITNWAR TTFRNEYGKH DFKNEESLSE EHASLLVRDS KPSKPFNVDD VLKFTFTGEK PIITGSTSTS SSN
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 668
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins. MED8 binds to the consensus sequence 5'-[AC][AG]GAAAT-3' in both the UAS of SUC2 and the DRS2 of HXK2.
基因功能參考文獻：
1. We identified Rgt1 as a transcription factor that, together with the Med8 protein, is essential for repression of the HXK2 gene in the absence of glucose. PMID: 15705057
2. Our results and published data support a positive role of the Med8/18/20 subcomplex in initiation-complex formation and suggest that the Mediator head contains a multipartite TBP-binding site that can be modulated by transcriptional activators. PMID: 16964259
3. Deletion of med8C, med18, or med20 causes similar changes in the yeast transcriptome. PMID: 18381891
亞細胞定位：

Nucleus.
蛋白家族：

Mediator complex subunit 8 family
數據庫鏈接：

KEGG: sce:YBR193C

STRING: 4932.YBR193C