Recombinant Saccharomyces cerevisiae Lariat debranching enzyme (DBR1)

中文名稱：

Recombinant Saccharomyces cerevisiae Lariat debranching enzyme(DBR1)
貨號：

CSB-YP006529SVG
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

Recombinant Saccharomyces cerevisiae Lariat debranching enzyme(DBR1)
貨號：

CSB-EP006529SVG
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

Recombinant Saccharomyces cerevisiae Lariat debranching enzyme(DBR1)
貨號：

CSB-EP006529SVG-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Saccharomyces cerevisiae Lariat debranching enzyme(DBR1)
貨號：

CSB-BP006529SVG
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Saccharomyces cerevisiae Lariat debranching enzyme(DBR1)
貨號：

CSB-MP006529SVG
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

DBR1
Uniprot No.：

P24309
別名：

DBR1; PRP26; YKL149C; YKL604; Lariat debranching enzyme; EC 3.1.-.-
種屬：

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
蛋白長度：

full length protein
表達區域：

1-405
氨基酸序列

MTKLRIAVQG CCHGQLNQIY KEVSRIHAKT PIDLLIILGD FQSIRDGQDF KSIAIPPKYQ RLGDFISYYN NEIEAPVPTI FIGGNHESMR HLMLLPHGGY VAKNIFYMGY SNVIWFKGIR IGSLSGIWKE WDFNKQRPDW NDLENNNWKA NIRNLYHVRI SDIAPLFMIK HRIDIMLSHD WPNGVVYHGD TKHLLKLKPF FEQDIKEGKL GSPVTWQLLR DLRPQWWLSA HLHVRFMASI KHNKRSHEPP NKSTSKTKKN NNEIDLDLSS DEDERSGIMN CQEENEYDSK YGETRFLALD KCLPRRRWLE ILEIEPDTSH ASWKDENHRM FWDPEFINNL VICQKNKNLL SNKPFNSVNW IELSQSNREE GRDIDWENYA IPAYTLDIQK DEVRQTKAFI SKFMT
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Cleaves the 2'-5' phosphodiester linkage at the branch point of lariat intron pre-mRNAs after splicing and converts them into linear molecules that are subsequently degraded. It thereby facilitates ribonucleotide turnover. It also participates in Ty1 retrovirus-like transposition via an RNA lariat intermediate in cDNA synthesis.
基因功能參考文獻：
1. Sequencing of lariat termini in S. cerevisiae reveals 5' splice sites, branch points, and novel splicing events. PMID: 26647463
2. data link three RNA catabolic enzymes, Rex4p, Rnt1p, and Dbr1p, to ITS1 processing and the relative production of 5.8SS and 5.8SL rRNA PMID: 15525710
3. Identification of 13 residues that are important for the intron debranching activity of Dbr1. PMID: 16275784
4. Dbr1p does not debranch an obligatory 2',5'-branched RNA linkage during the normal biological pathway of Ty1 retrotransposition. PMID: 17652136
亞細胞定位：

Nucleus. Cytoplasm.
蛋白家族：

Lariat debranching enzyme family
數據庫鏈接：

KEGG: sce:YKL149C

STRING: 4932.YKL149C