Recombinant Saccharomyces cerevisiae General control protein GCN4 (GCN4)

^{In
Stock}

中文名稱：

Recombinant Saccharomyces cerevisiae General control protein GCN4 (GCN4)
貨號：

CSB-EP365837SVGa0
說明書：

MSDS Datasheet
規格：

￥2328
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

GCN4
Uniprot No.：

P03069
別名：

Amino acid biosynthesis regulatory protein;General control protein GCN4
種屬：

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
蛋白長度：

Full Length
來源：

E.coli
分子量：

37.3 kDa
表達區域：

1-281aa
氨基酸序列

MSEYQPSLFALNPMGFSPLDGSKSTNENVSASTSTAKPMVGQLIFDKFIKTEEDPIIKQDTPSNLDFDFALPQTATAPDAKTVLPIPELDDAVVESFFSSSTDSTPMFEYENLEDNSKEWTSLFDNDIPVTTDDVSLADKAIESTEEVSLVPSNLEVSTTSFLPTPVLEDAKLTQTRKVKKPNSVVKKSHHVGKDDESRLDHLGVVAYNRKQRSIPLSPIVPESSDPAALKRARNTEAARRSRARKLQRMKQLEDKVEELLSKNYHLENEVARLKKLVGER
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Master transcriptional regulator that mediates the response to amino acid starvation. Binds variations of the DNA sequence 5'-ATGA[CG]TCAT-3' in canonical nucleosome-depleted 5'-positioned promoters, and also within coding sequences and 3' non-coding regions. During nutrient starvation (low or poor amino acid, carbon or purine sources), it activates genes required for amino acid biosynthesis and transport, autophagy, cofactor biosynthesis and transport, mitochondrial transport, and additional downstream transcription factors. Activates transcription by recruiting multiple coactivators, including the mediator complex, the SAGA complex, and the SWI/SNF complex, to enable assembly of the pre-initiation complex at core promoters.
基因功能參考文獻：
1. The down-regulation of the GCN4 expression (Gcn(-) phenotype) in the eIF5(G31R) mutant was not because of leaky scanning defects; rather was due to the utilization of upUUG initiation codons at the 5' regulatory region present between uORF1 and the main GCN4 ORF. PMID: 28385532
2. GCN4 is required for nitrogen catabolite repression-sensitive nuclear Gln3 localization. PMID: 28007891
3. Gcn4 sumoylation in limiting its association with chromatin. PMID: 27770033
4. Presented are molecular dynamics simulations reflecting a secondary structure of phopspho-GCN4 at different pH. PMID: 28230348
5. Reinitiation on GCN4 mRNA after translation of its four short uORFs is regulated by the sequence immediately following their respective stop codons. PMID: 26822200
6. Upstream-open reading frame 2 (uORF2) from the GCN4 mRNA leader resembles the well-established reinitiation (REI)-permissive uORF1 in its high REI-promoting activity. PMID: 24623812
7. our results suggest that the folding behavior of the well-studied GCN4 leucine-zipper domain is more complex than was previously appreciated. PMID: 23116373
8. Elucidated RNA binding interface of the GCN4 leucine zipper motif from yeast. Site-directed mutagenesis of the LZ-GCN4 RNA binding interface showed that substrate binding is facilitated by lysine and arginine side chains. PMID: 22374868
9. the combination of Gln3 and Gcn4 results in the constitution of a hybrid modulator which elicits a novel transcriptional response, not evoked when these modulators act in a non-combinatorial fashion. PMID: 21184740
10. Gcn4(L267S) variant carries an amino acid substitution in the third of four conserved leucines of the zipper dimerization domain. This abolished FLO11 expression and resulted in reduced transcriptional activity for amino acid biosynthetic genes. PMID: 21111745
11. GAAC is a major effector of the TOR pathway, with Gcn4p and Gln3p each inducing a similar number of genes during rapamycin treatment PMID: 20233714
12. interactions between the two nanorope-like assemblies are due to the hydrophobic residues on the leucine faces, which are also responsible for the dimer formation of the wild-type GCN4 leucine zipper. PMID: 20027625
13. eIF3b functions between 48S assembly and subunit joining to influence AUG recognition and reinitiation on GCN4 mRNA PMID: 14976554
14. Transcriptional activator Gcn4p recruits SAGA and Srb mediator to the upstream activation sequence of the promoter regions. PMID: 15831453
15. Site-specific photocrosslinkers positioned within the central transcription-activating region of yeast Gcn4 were used to identify three polypeptides in direct physical proximity to the activator during the process of transcription activation. PMID: 15866178
16. eIF5 is critical for the integrity of the scanning preinitiation complex and the accurate control of GCN4 translation. PMID: 15964804
17. Simultaneous recruitment of trans-activators by GCN4 stimulates multiple steps of transcription in vivo. PMID: 15964818
18. GCN4 expression is induced by sodium and has a role in controlling the accumulation of the 5' to 3' RNA degradation inhibitor, 3'-phosphoadenosine 5'-phosphate PMID: 16352596
19. large fraction of genes activated by Gcn4p are repressed by H3 and H4 amino termini under non-inducing conditions, indicating that one function of Gcn4p is to overcome chromatin-mediated repression that depends on histone tails PMID: 16461773
20. report here the design of a modified GCN4-bZIP DNA-binding protein with an azobenzene chromophore introduced between Cys residues at positions 262 and 269 (S262C, N269C) within the zipper domain PMID: 16681380
21. a set of native or nativelike tertiary interactions, distributed throughout the entire sequence, collectively stabilize the folding transition state of the GCN4 coiled coil. PMID: 16953584
22. Results describe the CPC1 regulon, the ortholog of Saccharomyces cerevisiae GCN4, by profiling transcription in wild-type and cpc-1 mutant strains. PMID: 17449655
23. LZGCN4 x-form is generally less structured in comparison with the classical GCN4-p1 coiled coil, but still retains a structured alpha-helical central core PMID: 17469817
24. Rpn13 protein and Rpn14 protein are involved in the efficient recognition of 26S proteasome for the proteolysis of ubiquitinated Gcn4 protein. PMID: 17499717
25. Gcn4 is required for the response to peroxide stress in the yeast Saccharomyces cerevisiae PMID: 18417611
26. Genetic epistasis analyses suggest that dietary restriction, reduced 60S subunit abundance, and Gcn4 activation extend yeast life span by similar mechanisms. PMID: 18423200
27. Results suggest that decreasing cargo proteins in the late endosome through impaired delivery or enhanced degradation is required to attenuate substantially transcriptional activation by Gcn4. PMID: 18794364
28. Adaptation to methylglyoxal was impaired in gcn4Delta cells, indicating the expression of certain genes regulated by Gcn4 to be important for the adaptive response to methylglyoxal. PMID: 18812164
29. 5,764 mRNAs, 54 metabolites, and 83 experimental (13)C-based reaction fluxes were quantified in continuous cultures of yeast under stress in the absence or presence of global regulator Gcn4p. PMID: 19346491
顯示更多

收起更多
亞細胞定位：

Nucleus.
蛋白家族：

BZIP family, GCN4 subfamily
數據庫鏈接：

KEGG: sce:YEL009C

STRING: 4932.YEL009C