Recombinant Saccharomyces cerevisiae DNA replication complex GINS protein PSF1 (PSF1)
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中文名稱:釀酒酵母PSF1重組蛋白
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貨號(hào):CSB-EP614228SVG-B
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說明書:
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規(guī)格:
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來源:E.coli
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共軛:Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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其他:
產(chǎn)品詳情
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純度:>85% (SDS-PAGE)
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基因名:PSF1
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Uniprot No.:
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別名:PSF1; YDR013W; PZA208; YD8119.18; DNA replication complex GINS protein PSF1; Partner of Sld five 1
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種屬:Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
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蛋白長(zhǎng)度:full length protein
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表達(dá)區(qū)域:1-208
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氨基酸序列MYGDLGNKLV LEAKRTKQLY ARSNQDVNLP MYHEDIIRNI LKEVSNLRKN TEYLKEQQQL GMLDDKVAKC QYFVTLLCME RNKRCLLAYQ RLRTDILDSM AWNNNGLDLM SSITFSQQDT NNLSHQEQEY LKEYCDLITD LKSGDLVDID LSGSLVPPSD VFIDVRVLKD AGEIQTEYGV FNLIKDSQFF VRQSDVERLI QQGYLQKI
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蛋白標(biāo)簽:Tag?type?will?be?determined?during?the?manufacturing?process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially. -
產(chǎn)品提供形式:Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
復(fù)溶:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
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儲(chǔ)存條件:Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質(zhì)期:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期:Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
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注意事項(xiàng):Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Datasheet :Please contact us to get it.
靶點(diǎn)詳情
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功能:Required for DNA replication. Functions as part of the GINS complex which plays an essential role in the initiation of DNA replication by binding to DNA replication origins and facilitating the assembly of the DNA replication machinery. Required for the chromatin binding of CDC45.
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基因功能參考文獻(xiàn):
- The association of Dpb2 with the B domain of Psf1 is required for initiation, and afterwards it links the leading strand DNA polymerase to the Cdc45-MCM-GINS helicase within the replisome. PMID: 23499531
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亞細(xì)胞定位:Nucleus. Note=Associates with autonomously replicating sequence (ARS) regions in S phase. This association requires SLD3 and DPB11.
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蛋白家族:GINS1/PSF1 family
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數(shù)據(jù)庫(kù)鏈接:
KEGG: sce:YDR013W
STRING: 4932.YDR013W
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