E. coli biotin ligase
(BirA) is highly specific in covalently attaching biotin to the 15
amino
acid AviTag peptide. This recombinant protein was biotinylated in
vivo
by AviTag-BirA technology, which method is BriA catalyzes amide
linkage
between the biotin and the specific lysine of the AviTag.
The tag type will
be
determined during production process. If you have specified tag
type, please tell us and we will develop the specified tag
preferentially.
產品提供形式:
Lyophilized
powder
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Note: We will
preferentially ship the format that we have in stock, however,
if you have any special requirement for the format, please
remark your requirement when placing the order, we will prepare
according to your demand.
復溶:
We recommend that this vial be briefly centrifuged
prior
to opening to bring the contents to the bottom. Please reconstitute
protein in deionized sterile water to a concentration of 0.1-1.0
mg/mL.We recommend to add 5-50% of glycerol (final concentration)
and
aliquot for long-term storage at -20℃/-80℃. Our default final
concentration of glycerol is 50%. Customers could use it as
reference.
儲存條件:
Store at -20°C/-80°C upon receipt, aliquoting is
necessary for
mutiple use. Avoid repeated freeze-thaw cycles.
保質期:
The shelf life is related to many factors, storage
state,
buffer ingredients, storage temperature and the stability of the
protein
itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C.
The
shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期:
Delivery time may
differ from different purchasing way or location, please kindly
consult your local distributors for specific delivery time.
Note: All of our
proteins are default shipped with normal blue ice packs, if you
request to ship with dry ice, please communicate with us in
advance
and extra fees will be charged.
注意事項:
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.
基因功能參考文獻:
findings indicate that unilateral cochlear ablation suppresses VGLUT1 expression in the central auditory pathways not only ipsilateral but also contralateral to the ablated side. PMID: 28238468
VGLUT1 was transiently down-regulated in ipsilateral dorsal root ganglia and contralateral spinal cord in a model of spared nerve injury. PMID: 27210824
In previously unstressed rats, vGluT1 siRNA significantly enhanced ACTH and corticosterone secretion. Compared with chronic variable stress (CVS) animals receiving the green fluorescent protein control vector, the vGluT1 siRNA further increased basal and stress-induced corticosterone release. Further analysis revealed enhanced adrenal responsiveness in CVS rats treated with vGluT1 siRNA. PMID: 28938481
results indicated that VGLUT1v exists in a functional state in rat photosensitive cells and is involved in glutamatergic chemical transmission PMID: 28188742
vGlut1 has a strong influence on the kinetics of retrieval of half of the known SV cargos and that specifically impairing the endocytosis PMID: 26224632
The results indicated that glutamatergic spinal trigeminal nucleus neurons sending their axons to the cerebellar cortical regions mainly express VGLUT1. PMID: 23380804
Following peripheral nerve injury, 62% of VGLUT1 synapses are lost on spinal motor neuron dendrites. PMID: 24599449
These results suggest that BDNF regulates VGLUT expression during development and its effect on VGLUT1 may contribute to enhance glutamate release in LTP PMID: 23326507
Up-regulation of VGLUT1 is important for development of presynaptic terminals in the cortex. PMID: 23226425
VGluT1 immunoreactions are localized within the cytoplasm for all sizes of trigeminal neurons, although predominantly in medium-large trigeminal neurons. PMID: 21957077
VGLUT1 labeling is concentrated in astrocytic processes from wild type, and not in VGLUT1 knockout hippocampus. PMID: 22009457
Expression of VGLUT2 precedes that of VGLUT1 during olfactory bulb morphogenesis, suggesting differential contribution of the two VGLUT subtypes to glutamate-mediated embryonic events. PMID: 21609737
After nerve injuries synapses are stripped from motoneurons, other excitatory and inhibitory inputs eventually recover but VGLUT1 synapses are permanently lost on the cell body (75-95% synaptic losses) and on the proximal 100 mum of dendrite (50% loss). PMID: 21832035
The VGLUT1 upstream promoter and the first intron, fused to the basal promoter, both support glutamatergic-specific expression in rat postrhinal cortex. PMID: 21172319
The vesicular glutamate transporter-1 (VGLUT1)promoter supports expression in VGLUT1-containing glutamatergic neurons in rat postrhinal cortex. PMID: 20849834
Most of the glutamatergic neurons that expressed glutamate vesicular transporter 1 also co-expressed choline acetyltransferase. PMID: 20632124
There are regionally significant correlations between VGLUT1 concentration and cognitive scores between different dementia groups and those with stroke who do not develop dementia. PMID: 21079182
observed a biphasic and bilateral change in the protein expression levels of both VGLUTs in the striatum of hemi-Parkinson rats indicative for a different and time-dependent change in glutamatergic neurotransmission from two types of striatal afferents PMID: 20450947
The study suggested that VGLUT enhances transmitter storage in nonglutamatergic neurons. PMID: 20519538
The presence of the glutamate markers VGLUT1 and VGLUT2 in distinct populations of peptide hormone-secreting hypophysial cells highly indicates the involvement of endogenous glutamate release in autocrine/paracrine regulatory mechanisms PMID: 20025917
These findings show the specific lingual pattern of distribution for VGluts and suggest that only VGlut 1 takes part in glutamatergic regulation of epithelial and taste cells within the tongue in physiological conditions. PMID: 19952853
VGLUT1 immunoreactivity was detected in the sensory endings on the equatorial and juxta-equatarial regions of intrafusal fibers as well as in many axon terminals within lamina IX of the spinal cord. PMID: 15157812
VGLUT1 provides a specific marker for glutamatergic neuronal elements in the septal hippocampus. PMID: 15224985
The developmental pattern of Vglut1 in the olivary nucleus was studied. PMID: 15714284
the coupling of zinc transporter 3 and Vglut1 transport mechanisms regulates neurotransmitter content in secretory vesicles PMID: 15860731
A newly proposed mechanism for altering the strength of glutamatergic synapses based on the recently identified vesicular glutamate transporter VGLUT1. PMID: 15987952
a high proportion of cardiac neurones appear to be glutamatergic, but differ from other vagal afferents in expressing vesicular glutamate transporter 1 PMID: 16084661
VGLUT1-ir was found in extrinsic as well as intrinsic innervation of the rat esophagus. PMID: 16231188
Overall, the discovery of endophilin as a partner for VGLUT1 in nerve terminals strongly suggests the existence of functional differences between VGLUT1 and -2 terminals in their abilities to replenish vesicle pools. PMID: 16606361
VGLUT1 and endophilins may interact to play an important role affecting the vesicular sequestration and synaptic release of glutamate. PMID: 16710756
Lamina I and IIo displayed a moderate density of small VGLUT1 varicosities at all spinal levels. Corticospinal tract axons displayed VGLUT1, indicating that the corticospinal tract is an important source of small VGLUT1 varicosities. PMID: 16786558
After stimulation, inhibition of the AP3 pathway prevents the full recovery of VGLUT1 by endocytosis, implicating the AP3 pathway specifically in compensatory endocytosis. PMID: 16815333
These data indicate that VGLUT1 is expressed in both the central axon terminals and the peripheral sensory endings of Vmes neurons, although no VGLUT1 immunoreactivity was detectable in the cell bodies of Vmes neurons in adult rats. PMID: 16856164
In situ hybridization with variant-specific probes showed expression of VGLUT1v in the inner segment layer of photoreceptor cells. PMID: 16987242
No significant change in either VGLUT1 or GlyT2 labeling of sacrocaudal motoneurons at any of the time points examined. PMID: 17134699
There was a striking loss of mossy cells during the latent period, demonstrated by a long-term decrease of VGLUT1 mRNA-containing hilar neurons and associated loss of VGLUT1-containing terminals in the dentate gyrus. PMID: 17503488
VGluT1 was localized to partial neurons of VG and to the putative primary afferent fibers innervating vestibular end-organs. PMID: 17612597
Histological analysis revealed that intensities of VGLUT1 hybridization signal and immunostaining drastically increase by postnatal day (P) 7 in pinal gland. PMID: 18291592
Results showed numerous VGLUT1 immunolabeled terminals in the central nucleus, lateral cortex and dorsal cortex, and Results showed numerous VGLUT1 and 2 immunolabeled terminals in the central nucleus, lateral cortex and dorsal cortex. PMID: 18436385
In rat retina, VGLUT1 was initially detected at postnatal day (P5) in photoreceptor terminals and P6 in bipolar terminals. PMID: 18482716
In the present study observed a higher expression of vglut1 mRNA in the left cingulate cortex. This suggests a hemispheric asymmetry in the strength of the cortico-subcortical glutamatergic system. PMID: 19626270
In cortical cultures and layer V cortical terminals VGLUT1 is coexpressed with vesicular transporter VGAT in the same population of synaptic vesicles. PMID: 19627441