Recombinant Rat Serine/threonine-protein kinase WNK1 (Wnk1), partial

中文名稱(chēng)：

大鼠Wnk1重組蛋白
貨號(hào)：

CSB-YP884914RA
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：
來(lái)源：

Yeast
其他：

中文名稱(chēng)：

大鼠Wnk1重組蛋白
貨號(hào)：

CSB-EP884914RA
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：
來(lái)源：

E.coli
其他：

中文名稱(chēng)：

大鼠Wnk1重組蛋白
貨號(hào)：

CSB-EP884914RA-B
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：
來(lái)源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱(chēng)：

大鼠Wnk1重組蛋白
貨號(hào)：

CSB-BP884914RA
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：
來(lái)源：

Baculovirus
其他：

中文名稱(chēng)：

大鼠Wnk1重組蛋白
貨號(hào)：

CSB-MP884914RA
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：
來(lái)源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

Wnk1
Uniprot No.：

Q9JIH7
別名：

Wnk1; Hsn2; Prkwnk1Serine/threonine-protein kinase WNK1; EC 2.7.11.1; Protein kinase lysine-deficient 1; Protein kinase with no lysine 1
種屬：

Rattus norvegicus (Rat)
蛋白長(zhǎng)度：

Partial
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4, SLC26A6 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition.; Dominant-negative regulator of the longer isoform 1. Does not have kinase activity, does not directly inhibit WNK4 and has no direct effect on sodium and chloride ion transport. Downregulates sodium-chloride cotransporter activity indirectly by inhibiting isoform 1, it associates with isoform 1 and attenuates its kinase activity. In kidney, may play an important role regulating sodium and potassium balance.
基因功能參考文獻(xiàn)：
1. these data provide insight into the mechanism regulating Cl(-) homeostasis in immature neurons, and suggest that WNK1-regulated changes in KCC2 phosphorylation contribute to the developmental excitatory-to-inhibitory GABA sequence. PMID: 26126716
2. WNK1 interacts with LINGO1 in oligodendrocytes. Knockdown WNK1 attenuated Nogo66-induced inhibition of OPC maturation. PMID: 25749374
3. The WNK1-AI domain has a circularly permuted topology relative to the OSR1-PF2 domain. PMID: 23376100
4. Data find that expression of the N termini of all four WNKs results in modest to strong activation of SGK1. PMID: 20525693
5. HSN2 is a nervous system-specific exon of the WNK1 gene (WNK1/HSN2 isoform). PMID: 18521183
6. WNK1 has a role in the sodium regulatory pathway of the distal PMID: 12671053
7. stimulation of KS-WNK1 expression might be an important element of aldosterone-induced Na(+) retention and hypertension PMID: 15583131
8. ROMK is antagonistically regulated by long and kidney-specific WNK1 isoforms PMID: 16428287
9. the effect of WNK1 on CFTR activity requires intact WNK1 kinase activity. Moreover WNK1 and WNK4 exhibit additive CFTR inhibition PMID: 17194447
10. a novel function of WNK1 in endothelial cells that is critical for angiogenesis and heart development PMID: 19644017
11. The kidney-specific WNK1 isoform suppresses thiazide-sensitive Na-Cl cotransport through the inhibition of full length WNK1 activity. PMID: 16204408
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亞細(xì)胞定位：

Cytoplasm.
蛋白家族：

Protein kinase superfamily, Ser/Thr protein kinase family, WNK subfamily
數(shù)據(jù)庫(kù)鏈接：

KEGG: rno:116477

STRING: 10116.ENSRNOP00000013355

UniGene: Rn.27409