Recombinant Rat Low-density lipoprotein receptor-related protein 2 (Lrp2), partial
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中文名稱：大鼠Lrp2重組蛋白
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貨號：CSB-EP013096RA-B
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說明書：
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規格：
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來源：E.coli
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共軛：Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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其他：
產品詳情
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純度：>85% (SDS-PAGE)
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基因名：
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Uniprot No.：
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別名：Lrp2; Low-density lipoprotein receptor-related protein 2; LRP-2; Glycoprotein 330; gp330; Megalin
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種屬：Rattus norvegicus (Rat)
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蛋白長度：Partial
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蛋白標簽：Tag?type?will?be?determined?during?the?manufacturing?process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially. -
產品提供形式：Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
復溶：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
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儲存條件：Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質期：The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期：Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
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注意事項：Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Datasheet ：Please contact us to get it.
產品評價
相關產品
靶點詳情
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功能：Multiligand endocytic receptor. Acts together with CUBN to mediate endocytosis of high-density lipoproteins. Mediates receptor-mediated uptake of polybasic drugs such as aprotinin, aminoglycosides and polymyxin B. In the kidney, mediates the tubular uptake and clearance of leptin. Also mediates transport of leptin across the blood-brain barrier through endocytosis at the choroid plexus epithelium. Endocytosis of leptin in neuronal cells is required for hypothalamic leptin signaling and leptin-mediated regulation of feeding and body weight. Mediates endocytosis and subsequent lysosomal degradation of CST3 in kidney proximal tubule cells. Mediates renal uptake of 25-hydroxyvitamin D3 in complex with the vitamin D3 transporter GC/DBP. Mediates renal uptake of metallothionein-bound heavy metals. Together with CUBN, mediates renal reabsorption of myoglobin. Mediates renal uptake and subsequent lysosomal degradation of APOM. Plays a role in kidney selenium homeostasis by mediating renal endocytosis of selenoprotein SEPP1. Mediates renal uptake of the antiapoptotic protein BIRC5/survivin which may be important for functional integrity of the kidney. Mediates renal uptake of matrix metalloproteinase MMP2 in complex with metalloproteinase inhibitor TIMP1. Mediates endocytosis of Sonic hedgehog protein N-product (ShhN), the active product of SHH. Also mediates ShhN transcytosis. In the embryonic neuroepithelium, mediates endocytic uptake and degradation of BMP4, is required for correct SHH localization in the ventral neural tube and plays a role in patterning of the ventral telencephalon. Required at the onset of neurulation to sequester SHH on the apical surface of neuroepithelial cells of the rostral diencephalon ventral midline and to control PTCH1-dependent uptake and intracellular trafficking of SHH. During neurulation, required in neuroepithelial cells for uptake of folate bound to the folate receptor FOLR1 which is necessary for neural tube closure. In the adult brain, negatively regulates BMP signaling in the subependymal zone which enables neurogenesis to proceed. In astrocytes, mediates endocytosis of ALB which is required for the synthesis of the neurotrophic factor oleic acid. Involved in neurite branching. During optic nerve development, required for SHH-mediated migration and proliferation of oligodendrocyte precursor cells. Mediates endocytic uptake and clearance of SHH in the retinal margin which protects retinal progenitor cells from mitogenic stimuli and keeps them quiescent. Plays a role in reproductive organ development by mediating uptake in reproductive tissues of androgen and estrogen bound to the sex hormone binding protein SHBG. Mediates endocytosis of angiotensin-2. Also mediates endocytosis of angiotensin 1-7. Binds to the complex composed of beta-amyloid protein 40 and CLU/APOJ and mediates its endocytosis and lysosomal degradation. Required for embryonic heart development. Required for normal hearing, possibly through interaction with estrogen in the inner ear.
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基因功能參考文獻：
- Data suggest that, in acute kidney injury (here, due to cadmium poisoning), kidney exhibits down-regulation of megalin receptor activity; up-regulation of NFkappaB, TLR2 (toll like receptor-2), and TLR4 (toll like receptor-4); and significant reduction in GSH (glutathione). PMID: 29286200
- Exocytosis-mediated urinary C-megalin excretion is associated with the development and progression of diabetic nephropathy in T2DM, particularly due to megalin-mediated lysosomal dysfunction in proximal tubules. PMID: 28289043
- Data suggest both LRP1 and LRP2 are necessary in growth cones for ligand- (zinc metallothionein IIa-)mediated chemotactic calcium-dependent signal transduction in sensory neurons, for axon guidance/neurogenesis, and for peripheral nerve regeneration. PMID: 26598525
- Megalin-mediated B12 endocytosis in renal tubules may be regulated by methylmalonic acid. PMID: 26248135
- The inhibition of megalin/cubilin or lysosomal cathepsin B reduced albuminuria-induced NLRP3 inflammasome activation. PMID: 26025362
- ARH knockdown leads to fast recycling of megalin via a Rab35 pathway. PMID: 23836931
- Dietary resistant starch normalized megalin and Dab2 mRNA overexpression in diabetic rats. PMID: 23677864
- megalin-dependent endocytosis has a role along the proximal nephron in the intrarenal renin angiotensin system PMID: 20966072
- Amino acids 107-136 of megalin-CT containing the second NPXY-like motif are critical for apical sorting and targeting, whereas the regions containing the first and third NPXY motifs are required for efficient endocytosis. PMID: 12519751
- megalin functions for endocytosis of AGE via an indirect mechanism. PMID: 12707383
- endocytic receptors megalin and cubilin are involved in renal reabsorption of myoglobin PMID: 12724130
- Because thyroglobulin binding to megalin is greatest at acidic pH, it is possible that TG does not dissociate from megalin in the lysosomal pathway. However, the pH-dependence of TG binding to megalin does not account for much of transcytosis. PMID: 12809172
- Megalin is an endocytic receptor that binds multiple ligands and is essential for many physiological processes such as brain development PMID: 14528014
- megalin has a role in thyroid homeostasis with possible implications in thyroid diseases PMID: 14657389
- Evidence is provided in a rat model that a megalin N-terminal domain includes B and T cell epitopes sufficient for a pathogenic autoimmune response and that a native-like conformation and glycosylation are essential for induction of Heymann nephritis. PMID: 14764706
- the hinge SCKKSCC region of metallothionein as a critical site for megalin(lrp2) binding PMID: 15126248
- These data suggest that light chains bind the tandem megalin-cubilin complex. PMID: 15286052
- Cubilin expression at later developmental stages of the rodent embryo and its correlation to that of megalin was investigated. PMID: 16027047
- Megalin mediates IGF-I-induced clearance of Abeta and is involved in IGF-I transport into brain. Attenuated IGF-I/megalin input may contribute to increased risk of neurodegeneration, including late-onset Alzheimer's disease. PMID: 16306401
- In nephrotic rats, the rate of uptake of albumin by the proximal tubule (PT) is decreased. This is consistent with reduced expression of clathrin, megalin, and vacuolar H(+)-ATPase A subunit, proteins that are critical components of PT endocytosis. PMID: 17228368
- The solution structure of the twelfth cysteine-rich ligand-binding repeat with class A motif found in megalin features two short beta-strands and two helical turns. PMID: 17245526
- The present data indicate that leptin represents a novel megalin ligand of importance in the levels and therapeutic actions of leptin into the brain. PMID: 17324488
- Significant amounts of megalin and cubilin were present in the glomerular cells in addition to the proximal tubular cells. The quantity of these receptors progressively increased in the glomerulus with age. PMID: 17453355
- Upregulation of B7.1 with the downregulation of GLEPP1, Wilms' tumor gene (WT1), megalin, and vascular endothelial growth factor started early and persisted through the course of primary glomerular disease PMID: 17457373
- Three distinct regions of cubilin bind to megalin and its interactions with both megalin and amnionless are essential for its intracellular stability. PMID: 17990981
- Astrocytes express megalin, an endocytic receptor for multiple ligands including albumin; megalin is required for albumin binding and internalization in the astrocyte. PMID: 18466341
- There is AT(1A)R- and insulin-mediated competitive signaling cross talk to regulate megalin expression in cultured proximal tubule cells. PMID: 18927221
- cubilin and megalin constitute a dual-receptor complex facilitating the function of each other in the inner ear PMID: 19202329
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亞細胞定位：Apical cell membrane; Single-pass type I membrane protein. Endosome lumen. Membrane, clathrin-coated pit. Cell projection, dendrite. Cell projection, axon.
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蛋白家族：LDLR family
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組織特異性：In the inner ear, expressed in the lumen of the endolymphatic sac where it localizes to macrophage-like cells as well as to mitochondria-rich and ribosome-rich epithelial cells (at protein level). In the inner ear, expressed in marginal cells of the stria
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