Recombinant Rat Kalirin (Kalrn), partial

中文名稱：

大鼠Kalrn重組蛋白
貨號：

CSB-YP011981RA
說明書：

MSDS Datasheet
規(guī)格：
來源：

Yeast
其他：

中文名稱：

大鼠Kalrn重組蛋白
貨號：

CSB-EP011981RA
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
其他：

中文名稱：

大鼠Kalrn重組蛋白
貨號：

CSB-EP011981RA-B
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

大鼠Kalrn重組蛋白
貨號：

CSB-BP011981RA
說明書：

MSDS Datasheet
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

大鼠Kalrn重組蛋白
貨號：

CSB-MP011981RA
說明書：

MSDS Datasheet
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價
靶點詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

Kalrn
Uniprot No.：

P97924
別名：

Kalrn; Duo; Hapip; Kalirin; EC 2.7.11.1; Huntingtin-associated protein-interacting protein; PAM COOH-terminal interactor protein 10; P-CIP10; Protein Duo; Serine/threonine-protein kinase with Dbl- and pleckstrin homology domain
種屬：

Rattus norvegicus (Rat)
蛋白長度：

Partial
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

Promotes the exchange of GDP by GTP. Activates specific Rho GTPase family members, thereby inducing various signaling mechanisms that regulate neuronal shape, growth, and plasticity, through their effects on the actin cytoskeleton. Induces lamellipodia independent of its GEF activity. Isoforms 1 and 7 are necessary for neuronal development and axonal outgrowth. Isoform 6 is required for dendritic spine formation.
基因功能參考文獻(xiàn)：
1. Mass spectrometry was used to quantify phosphorylation at 26 sites in Kal7 from individual adult rat nucleus accumbens and prefrontal cortex before and after exposure to acute or chronic cocaine. Region- and isoform-specific phosphorylation was observed along with region-specific effects of cocaine on Kal7 phosphorylation. Calpain cleaved Kal7 at 2 sites, separating N-terminal domain and PDZ binding motif from GEF domain. PMID: 28418645
2. These results suggest that kalirin and Rac1 guanine nucleotide exchange factor play important roles in FSTL-1-mediated glucose regulation in skeletal muscle cells. PMID: 27989699
3. Chronic unpredictable mild stress alters structural plasticity in the orbitofrontal cortex and is associated with decreased expression of kalirin. PMID: 26921771
4. Results demonstrate that both endogenous kalirin-9 and endogenous kalirin-12 contribute to dendritic maturation in early development. PMID: 25146373
5. The effects of NRG1 and erbB4 on spines were mediated by the RacGEF kalirin PMID: 23742124
6. Knock-down of kalirin-7 abolishes AMPA receptor and dendritic spine upregulation normally seen during cocaine withdrawal. PMID: 23825406
7. The rigidity and flexibility of the nine spectrin repeats of Kal7, which separate its essential N-terminal Sec14p domain from its catalytic domain, play an essential role in its contribution to the formation and function of dendritic spines. PMID: 22738176
8. our findings indicate that kalirin-7 is differentially compartmentalized in spines as a function of both synapse morphology and spine size. PMID: 22458534
9. Estradiol treatment of cultured hippocampal neurons increased Kal7 levels at the postsynaptic side of excitatory synapses PMID: 20333733
10. results explain why Rac1 and its activator (Kal-7) serve as important mediators of spine enlargement and why constitutive Rac1 activation decreases spine size PMID: 20139976
11. Reduced expression of Kalirin in CA1 hippocampal neurons resulted in a reduction in linear spine density, with dispersion of postsynaptic density markers and elimination of presynaptic endings PMID: 14627644
12. we have identified multiple transcriptional start sites in rats and humans. These multiple transcriptional start sites result in full-length Kalirin transcripts possessing different 5' ends encoding proteins with differing amino termini PMID: 14742910
13. Data demonstrate that Kalirin binds to and colocalizes with the TrkA neurotrophin receptor in neurons. PMID: 15923627
14. a novel molecular mechanism whereby intramolecular and intermolecular Kalirin SH3 domain/ligand interactions modulate GEF activity, a regulatory mechanism that is likely used by other RhoGEF family members PMID: 16644733
15. NMDA receptor activation in pyramidal neurons causes CaMKII-dependent phosphorylation of kalirin-7 at threonine 95, regulating its GEF activity, leading to activation of small GTPase Rac1 and rapid enlargement of existing spines. PMID: 18031682
16. Sec14p and N-terminal spectrin-like domains of Kalirin play critical roles in distinguishing the actions of full-length and Delta-Kalirin proteins. PMID: 18585704
17. Kalirin9, a dual RhoGEF, binds p75 directly and regulates p75-Nogo receptor-dependent RhoA activation and neurite inhibition in response to myelin-associated glycoprotein. PMID: 18625710
18. The presence of Thr1590 in each major Kalirin isoform would allow Cdk5 to regulate Kalirin function throughout development PMID: 18628310
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亞細(xì)胞定位：

Cytoplasm. Cytoplasm, cytoskeleton. Note=Isoform 6 is largely associated with synaptosomal membranes and to punctate structures in cortical neurons. Isoforms 1 and 7 are expressed in neuronal cell bodies, isoform 7 is also found in neuronal processes.
蛋白家族：

Protein kinase superfamily, CAMK Ser/Thr protein kinase family
組織特異性：

Highly expressed in the brain and nervous system. Isoform 6 is highly enriched in the postsynaptic density fraction of the cerebral cortex.
數(shù)據(jù)庫鏈接：

KEGG: rno:84009

STRING: 10116.ENSRNOP00000039072

UniGene: Rn.87882