Recombinant Rat Glycogen synthase kinase-3 beta (Gsk3b)
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中文名稱：大鼠Gsk3b重組蛋白
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貨號：CSB-EP009963RA-B
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說明書：
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規格：
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來源：E.coli
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共軛：Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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其他：
產品詳情
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純度：>85% (SDS-PAGE)
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基因名：
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Uniprot No.：
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別名：Gsk3bGlycogen synthase kinase-3 beta; GSK-3 beta; EC 2.7.11.26; Factor A; FA; Serine/threonine-protein kinase GSK3B; EC 2.7.11.1
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種屬：Rattus norvegicus (Rat)
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蛋白長度：Full length protein
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表達區域：1-420
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氨基酸序列MSGRPRTTSF AESCKPVQQP SAFGSMKVSR DKDGSKVTTV VATPGQGPDR PQEVSYTDTK VIGNGSFGVV YQAKLCDSGE LVAIKKVLQD KRFKNRELQI MRKLDHCNIV RLRYFFYSSG EKKDEVYLNL VLDYVPETVY RVARHYSRAK QTLPVIYVKL YMYQLFRSLA YIHSFGICHR DIKPQNLLLD PDTAVLKLCD FGSAKQLVRG EPNVSYICSR YYRAPELIFG ATDYTSSIDM WSAGCVLAEL LLGQPIFPGD SGVDQLVEII KVLGTPTREQ IREMNPNYTE FKFPQIKAHP WTKVFRPRTP PEAIALCSRL LEYTPTARLT PLEACAHSFF DELRDPNVKL PNGRDTPALF NFTTQELSSN PPLATILIPP HARIQAAASP PANATAASDT NAGDRGQTNN AASASASNST
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蛋白標簽：Tag?type?will?be?determined?during?the?manufacturing?process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially. -
產品提供形式：Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
復溶：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
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儲存條件：Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質期：The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期：Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
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注意事項：Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Datasheet ：Please contact us to get it.
產品評價
問答及客戶評論
■ 常見問題解答
Q:
Is the protein active form? Please let me know whether the expression sequence is included the active part.
I know you don't measure the avtivity.
A:
Very nice to receive your inquiry.
Yes, as you already know, we haven't tested the activity of these proteins.
However, as what we provide is the full length protein, and it's purified under very mild condition, in theory, they are very likely to be ative.
Probably, you can consider order a small size to have a try.
Yes, as you already know, we haven't tested the activity of these proteins.
However, as what we provide is the full length protein, and it's purified under very mild condition, in theory, they are very likely to be ative.
Probably, you can consider order a small size to have a try.
靶點詳情
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功能：Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, DPYSL2/CRMP2, JUN, NFATC1/NFATC, MAPT/TAU and MACF1. Requires primed phosphorylation of the majority of its substrates. In skeletal muscle, contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. May also mediate the development of insulin resistance by regulating activation of transcription factors. Regulates protein synthesis by controlling the activity of initiation factor 2B (EIF2BE/EIF2B5) in the same manner as glycogen synthase. In Wnt signaling, GSK3B forms a multimeric complex with APC, AXIN1 and CTNNB1/beta-catenin and phosphorylates the N-terminus of CTNNB1 leading to its degradation mediated by ubiquitin/proteasomes. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates NFATC1/NFATC on conserved serine residues promoting NFATC1/NFATC nuclear export, shutting off NFATC1/NFATC gene regulation, and thereby opposing the action of calcineurin. Phosphorylates MAPT/TAU on 'Thr-548', decreasing significantly MAPT/TAU ability to bind and stabilize microtubules. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Phosphorylates MACF1, inhibiting its binding to microtubules which is critical for its role in bulge stem cell migration and skin wound repair. Probably regulates NF-kappa-B (NFKB1) at the transcriptional level and is required for the NF-kappa-B-mediated anti-apoptotic response to TNF-alpha (TNF/TNFA). Negatively regulates replication in pancreatic beta-cells, resulting in apoptosis, loss of beta-cells. Through phosphorylation of the anti-apoptotic protein MCL1, may control cell apoptosis in response to growth factors deprivation. Phosphorylates MUC1 in breast cancer cells, decreasing the interaction of MUC1 with CTNNB1/beta-catenin. Is necessary for the establishment of neuronal polarity and axon outgrowth. Phosphorylates MARK2, leading to inhibit its activity. Phosphorylates SIK1 at 'Thr-182', leading to sustain its activity. Phosphorylates ZC3HAV1 which enhances its antiviral activity. Phosphorylates SFPQ upon T-cell activation. Phosphorylates SNAI1, leading to its BTRC-triggered ubiquitination and proteasomal degradation. Phosphorylates NR1D1 st 'Ser-55' and 'Ser-59' and stabilizes it by protecting it from proteasomal degradation. Regulates the circadian clock via phosphorylation of the major clock components including ARNTL/BMAL1, CLOCK and PER2. Phosphorylates CLOCK AT 'Ser-427' and targets it for proteasomal degradation. Phosphorylates ARNTL/BMAL1 at 'Ser-17' and 'Ser-21' and primes it for ubiquitination and proteasomal degradation. Phosphorylates OGT at 'Ser-3' or 'Ser-4' which positively regulates its activity. Regulates the circadian rhythmicity of hippocampal long-term potentiation and ARNTL/BMLA1 and PER2 expression. Acts as a regulator of autophagy by mediating phosphorylation of KAT5/TIP60 under starvation conditions, leading to activate KAT5/TIP60 acetyltransferase activity and promote acetylation of key autophagy regulators, such as ULK1 and RUBCNL/Pacer. Negatively regulates extrinsic apoptotic signaling pathway via death domain receptors. Promotes the formation of an anti-apoptotic complex, made of DDX3X, BRIC2 and GSK3B, at death receptors, including TNFRSF10B. The anti-apoptotic function is most effective with weak apoptotic signals and can be overcome by stronger stimulation.
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基因功能參考文獻：
- the findings obtained from the study support the concept that sevoflurane preconditioning confers cardioprotection against myocardial I/R injury and GSK-3beta/beta-catenin signaling activation mediated by TDZD-8 as a novel target to prolong cardioprotection by sevoflurane anaesthesia. PMID: 29987974
- GSK-3beta/beta-catenin signaling contributes to the bone remodeling induced by orthodontic forces PMID: 29224185
- that PI3K/Akt-dependent GSK-3beta signaling and subsequent expression of NeuroD were involved in the neurogenesis elicited by cerebral ischemia PMID: 27866373
- Activation of GSK-3 disrupts cholinergic homoeostasis in nucleus basalis of Meynert and frontal cortex of rats. PMID: 28656644
- found that inactivation of glycogen synthase kinase-3 beta (GSK3beta) resulting from activation of protein kinase C alpha (PKCalpha) is involved in the nNOS-mediated neuronal differentiation PMID: 27624386
- By down-regulating the PI3 K/AKT/GSK3beta pathway. PMID: 28810530
- This is the first time a comprehensive subset of proteins involved in FGF inhibitory response is defined. We were able to identify a number of targets and specifically discover GSK3B as a novel key mediator of FGF inhibitory response in chondrocytes. PMID: 28298517
- These results indicate that GSK-3beta inhibition protects the blood-brain barrier and attenuates early ischemia-reperfusion stroke injury PMID: 28553165
- PINCH/GSK3beta/ERK pathway induced in kidney in unilateral ureteral obstruction PMID: 27771700
- GSK-3beta inactivation suppresses osteolysis through regulating both osteoblast and osteoclast differentiation in a rat model of instability-induced osteolysis. PMID: 28731198
- The chemopreventive and anti-neoplastic effects of COX-2 inhibitors in the early stage of colon cancer are due to promotion apoptosis and over-expression of GSK-3beta while down-regulating the PI3-K/Akt oncogenic pathway. PMID: 28718684
- results suggest that silencing of GSK3beta in the Nuc. Accumbens increases depression- and addiction-related behavior. PMID: 28126496
- Astrocytes attenuate propofol-induced neurotoxicity through BDNF-mediated Akt/glycogen synthase kinase 3beta/mitochondrial fission pathway. PMID: 28622174
- activation of estrogen receptor beta may protect against brain ischemia-induced BBB disruption by inhibiting GSK3beta-mediated signaling PMID: 28440874
- Pharmacological reperfusion treatment with insulin and GSK3B inhibitor interferes with the cardioprotection afforded by myocardial ischemic postconditioning. PMID: 28276718
- Data suggest that ETA receptor antagonism is a mediator to attenuate sympathetic hyperinnervation probably through restoration of PI3K/Akt/GSK-3beta/ROS signaling pathway, a potential pharmacological target for arrhythmias after infarction. PMID: 27991911
- This study suggests that breviscapine inhibits the GSK3beta signaling pathway to promote neurobehavioral function following neurotrauma. These events may provide a new insight into the mechanism of breviscapine treating brain injury. PMID: 28143715
- mediates the development of cardiac hypertrophy by promoting GSK-3beta phosphorylation. PMID: 27545607
- I/R injuryresulted in overexpression of GSK-3b activity while reduced significantly the level of BDNF. However, lithium administration significantly inactivated GSK-3b and improved the BDNF level along with increased number of healthy hippocampal neurons ; lithium attenuated the levels of oxidative stress and pro-inflammatory cycytokines TNF-a level PMID: 26554621
- results indicate that mechanical stress-regulated osteogenesis and adipogenesis of rat BMSCs are mediated, at least in part, by the PI3K/Akt/GSK-3beta/beta-catenin signaling pathway. PMID: 28286769
- after the pectin treatment, phosphorylated Akt expression was upregulated and GSK3b expression was downregulated, indicating that the potential anti-diabetic mechanism of citrus pectin might occur through regulation of the PI3K/Akt signaling pathway PMID: 27164497
- expression of GSK-3beta in the dorsal root ganglia following peripheral nerve injury PMID: 27125978
- Synthesis of Novel Oxazolo[4,5-b]pyridine-2-one based 1,2,3-triazoles as Glycogen Synthase Kinase-3beta Inhibitors with Anti-inflammatory Potential. PMID: 26804375
- Rosuvastatin prevents myocardial ischemia-reperfusion injury by inducing phosphorylation of PI3K-Akt and GSK-3beta, preventing oxidative stress and subsequent inhibition of mPTP opening. PMID: 27580017
- results show for the first time that GSK-3beta can influence cerebral ischemia/reperfusion injury. PMID: 27019634
- GSK3beta plays an important role in regulating the proliferation and migration of Bone mesenchymal stem cells by inhibiting cyclin D1 and CXCR-4 under high glucose conditions. PMID: 25840567
- TNF-alpha and RANKL can suppress the proliferation of osteoblasts while had little effect on osteoclast proliferation; they also can promote the GSK-3beta gene expression PMID: 26722385
- indicate that GSK-3 activation mediates the olfactory deficits to the hippocampus, and targeting GSK-3 blocks the pathological connection PMID: 25367884
- Salidroside hase cardioprotective effects in rats subjected to LPS-induced sepsis, probably through regulation of IGF-1/PI3K/Akt/GSK-3beta signaling. PMID: 26104971
- Exploration of the molecular causes of enhanced cardiac hypertrophy revealed significant activation of beta-catenin/GSK-3beta signaling, whereas MAPK and MKL1/serum-response factor pathways were inhibited. PMID: 26719331
- diabetes during pregnancy up-regulates GSK-3beta mRNA, protein and phosphorylation levels in the developing hippocampi. PMID: 26242887
- The ILK pathway appears to play a significant role in memory and synaptic plasticity impairments in FASD rats. These impairments appear to be mediated by reduced GSK3beta regulation PMID: 26305322
- depressive-like behavior inversely correlated with serotonin-induced phosphorylation of GSK3beta in the subgranular neurons of the dentate gyrus. PMID: 25169083
- GSK-3beta suppresses the proliferation of hepatic oval cells by modulating the Wnt/beta-catenin signaling pathway. PMID: 25661318
- Early PI3K mediated activation of GSK-3beta attenuated quisqualic acid-induced dorsal root ganglia neurite outgrowth and prevented the development of at-level dysesthesias. PMID: 26093674
- GSK3beta is activated and p-LRP6 is down-regulated in kidneys of diabetic rats. These abnormalities were prevented by treatment with green tea. PMID: 25655048
- data demonstrate that IPC exerts protective effects against liver injury induced by I/R and activates the GSK-3beta/beta-catenin signaling pathway. PMID: 25813855
- GSK-3beta as a key negative effector of neural stem cell survival downstream of insulin signaling. PMID: 25986948
- GSK-3[beta] inhibition has an effect on rat liver ischemia-reperfusion and thermal injury PMID: 25394244
- Results that GSK3beta could regulate BDNF-dependent TrkB endocytosis through phosphorylating Ser-774 residue on Dyn1 and therefore influence BDNF-induced downstream signaling PMID: 25484286
- These data provide evidence that dysregulation of GSK3beta is a contributing factor in the molecular basis of innate dysfunction and susceptibility of diabetic host to melioidosis infection. PMID: 25801253
- Inhibition of neural progenitor cell proliferation induced by low concentration of MeHg was associated with up-regulation of GSK-3beta at the early stage and subsequent degeneration of cyclin E. PMID: 26184774
- Limb ischemic preconditioning protects against contrast-induced acute kidney injury in rats via phosphorylation of GSK-3beta. PMID: 25451640
- Results show that downregulation of GSK3 promotes synapse formation in Drosophila neurons. However in rats hippocampal neurons, GSK3 inhibition yields to decrease of synapses in young neurons culture and the opposite in aged culture. PMID: 25764078
- GSK3 is involved in the formation of leading processes and in the migration of medial ganglionic eminence neurons. PMID: 25646586
- Enhanced GSK-3beta activity within podocytes under diabetic conditions is associated with podocyte loss in diabetic nephropathy. PMID: 25284613
- identified GSK3beta as a profound positive regulator of oligodendrocyte precursor cells differentiation PMID: 24691545
- HS could attenuate neuronal apoptosis in early brain injury and improve the neurofunctional outcome after rachnoid hemorrhage, partially via the Akt/GSK3beta pathway. PMID: 24763696
- Accelerated recovery of mitochondrial membrane potential by GSK-3beta inactivation affords cardiomyocytes protection from oxidant-induced necrosis. PMID: 25390651
- The inhibition of GSK3beta attenuates renal disorders. PMID: 25323773
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亞細胞定位：Cytoplasm. Nucleus. Membrane. Cell membrane.
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蛋白家族：Protein kinase superfamily, CMGC Ser/Thr protein kinase family, GSK-3 subfamily
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