Recombinant Rat Cholesterol 7-alpha-monooxygenase (Cyp7a1)

中文名稱：

Recombinant Rat Cholesterol 7-alpha-monooxygenase(Cyp7a1)
貨號：

CSB-YP006460RA
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

Recombinant Rat Cholesterol 7-alpha-monooxygenase(Cyp7a1)
貨號：

CSB-EP006460RA
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

Recombinant Rat Cholesterol 7-alpha-monooxygenase(Cyp7a1)
貨號：

CSB-EP006460RA-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Rat Cholesterol 7-alpha-monooxygenase(Cyp7a1)
貨號：

CSB-BP006460RA
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Rat Cholesterol 7-alpha-monooxygenase(Cyp7a1)
貨號：

CSB-MP006460RA
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Cyp7a1
Uniprot No.：

P18125
別名：

Cyp7a1; Cyp7Cytochrome P450 7A1; 24-hydroxycholesterol 7-alpha-hydroxylase; EC 1.14.14.26; CYPVII; Cholesterol 7-alpha-hydroxylase; Cholesterol 7-alpha-monooxygenase; EC 1.14.14.23
種屬：

Rattus norvegicus (Rat)
蛋白長度：

full length protein
表達區域：

1-503
氨基酸序列

MMTISLIWGI AVLVSCCIWF IVGIRRRKAG EPPLENGLIP YLGCALKFGS NPLEFLRANQ RKHGHVFTCK LMGKYVHFIT NSLSYHKVLC HGKYFDWKKF HYTTSAKAFG HRSIDPNDGN TTENINNTFT KTLQGDALCS LSEAMMQNLQ SVMRPPGLPK SKSNAWVTEG MYAFCYRVMF EAGYLTLFGR DISKTDTQKA LILNNLDNFK QFDQVFPALV AGLPIHLFKT AHKAREKLAE GLKHKNLCVR DQVSELIRLR MFLNDTLSTF DDMEKAKTHL AILWASQANT IPATFWSLFQ MIRSPEAMKA ASEEVSGALQ SAGQELSSGG SAIYLDQVQL NDLPVLDSII KEALRLSSAS LNIRTAKEDF TLHLEDGSYN IRKDDMIALY PQLMHLDPEI YPDPLTFKYD RYLDESGKAK TTFYSNGNKL KCFYMPFGSG ATICPGRLFA VQEIKQFLIL MLSCFELEFV ESQVKCPPLD QSRAGLGILP PLHDIEFKYK LKH
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

A cytochrome P450 monooxygenase involved in the metabolism of endogenous cholesterol and its oxygenated derivatives (oxysterols). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). Functions as a critical regulatory enzyme of bile acid biosynthesis and cholesterol homeostasis. Catalyzes the hydroxylation of carbon hydrogen bond at 7-alpha position of cholesterol, a rate-limiting step in cholesterol catabolism and bile acid biosynthesis. 7-alpha hydroxylates several oxysterols, including 4beta-hydroxycholesterol and 24-hydroxycholesterol. Catalyzes the oxidation of the 7,8 double bond of 7-dehydrocholesterol and lathosterol with direct and predominant formation of the 7-keto derivatives.
基因功能參考文獻：
1. lnc-HC negatively regulates cholesterol metabolism within hepatocytes through physical interaction with hnRNPA2B1. By further binding to the target messenger RNA of Cyp7a1 or Abca1, the lnc-HC-hnRNPA2B1 complex decreases expressions of the two genes that are implicated in cellular cholesterol excretion. PMID: 26663205
2. Administration of glucocorticoids (GCs) enhanced liver bile acid uptake by increasing the expression of the major hepatocyte basolateral bile transporter (Ntcp) in rats. The reduced expression of a bile acid synthesis rate-controlling enzyme, Cyp7a1, suggests that treatment with GCs suppressed hepatic bile acid synthesis. PMID: 27133208
3. Dietary fats and glucomannan plus spirulina added to pork significantly alters Cyp7a1 expression in Zucker rats. PMID: 26475369
4. Ginsenosides promoted liver cholesterol metabolism due to upregulation of CYP7A1. PMID: 23108811
5. Portal blood FGF15/19 levels remain unchanged while the corresponding ileal mRNA and liver CYP7A1 levels change significantly in cholesterol fed rats. PMID: 23852734
6. Capsaicinoids consumption down-regulated the mRNA levels of HMG-CoA reductase and CYP7A1 in the cholesterol-free diet group but up-regulated the CYP7A1 level in the cholesterol-enriched diet group. PMID: 23617777
7. insulin-dependent suppression results in impairment of the hepatic cholesterol/bile acid metabolisms PMID: 21817852
8. Maternal protein restriction elevates cholesterol in adult rat offspring due to repressive changes in histone modifications at the cholesterol 7alpha-hydroxylase promoter PMID: 21372147
9. Cytochrome P450 7A1 cholesterol 7alpha-hydroxylation: individual reaction steps in the catalytic cycle and rate-limiting ferric iron reduction. PMID: 21147774
10. essential for heart and skeletal muscle function PMID: 20215401
11. The mRNA and protein expressions of SHP and CYP7A1 were increased in livers of pregnant rats with intrahepatic cholestasis. PMID: 18578998
12. Cyp7a1 activity is modified after chronic ingestion of depleted uranium in the rat. PMID: 20362056
13. The increased recruitment of LXRalpha, a Cyp7a1 stimulatory pathway, and decreased expression of FGF15 and phosphorylated Erk1/2, a Cyp7a1 repressive pathway, combined to increase Cyp7a1 expression during lactation. PMID: 19957370
14. LXR alpha is the dominant regulator of CYP7A1 transcription PMID: 12054605
15. transcription suppressed by alpha1-antitrypsin peptide via interaction with AFP PMID: 12223476
16. Data reveal a fundamental difference in the regulation of CYP7A1 in rodent and human hepatocytes. PMID: 12554795
17. ARP-1/COUP-TFII acts as a transcription activator of CYP7A1. PMID: 12777384
18. results suggest that the mechanisms of regulation of these bile acid metabolizing enzymes are significantly different during liver regeneration PMID: 16081591
19. These results suggest that the cyp7A1 gene plays an important role in development of hypercholesterolemia in Prague hereditary hypercholesterolemic rats. PMID: 18226361
20. Increased Cyp7a1 expression inlactating rats provides a mechanism for the increase in the bile acid pool. PMID: 18292185
21. The effect of osthole on protein expression of PPARalpha/gamma and related target molecules such as CYP7A and DGAT protein expression in the liver of hyperlipidemic fatty liver rats. PMID: 18696335
22. A relationship between the decreased expression of hepatic CYP7A1 mRNA and protein and dilated ER may contribute to hypercholesterolemia in chronic hypothyroidism. PMID: 19788618
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亞細胞定位：

Endoplasmic reticulum membrane; Single-pass membrane protein. Microsome membrane; Single-pass membrane protein.
蛋白家族：

Cytochrome P450 family
組織特異性：

Detected in liver (at protein level). Liver.
數據庫鏈接：

KEGG: rno:25428

STRING: 10116.ENSRNOP00000012819

UniGene: Rn.10737