Recombinant Pyrococcus kodakaraensis Probable L-threonine 3-dehydrogenase (tdh)

中文名稱：

Recombinant Pyrococcus kodakaraensis Probable L-threonine 3-dehydrogenase(tdh)
貨號：

CSB-YP702481FHY
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

Recombinant Pyrococcus kodakaraensis Probable L-threonine 3-dehydrogenase(tdh)
貨號：

CSB-EP702481FHY
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

Recombinant Pyrococcus kodakaraensis Probable L-threonine 3-dehydrogenase(tdh)
貨號：

CSB-EP702481FHY-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Pyrococcus kodakaraensis Probable L-threonine 3-dehydrogenase(tdh)
貨號：

CSB-BP702481FHY
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Pyrococcus kodakaraensis Probable L-threonine 3-dehydrogenase(tdh)
貨號：

CSB-MP702481FHY
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

tdh
Uniprot No.：

Q5JI69
別名：

tdh; TK0916; L-threonine 3-dehydrogenase; TDH; EC 1.1.1.103; L-threonine dehydrogenase
種屬：

Pyrococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Thermococcus kodakaraensis (strain KOD1))
蛋白長度：

full length protein
表達區域：

1-350
氨基酸序列

MAEKMQAIMK TKPAYGAELV EVDVPKPGPG EVLIKVLATS ICGTDLHIYE WNEWAQSRIK PPQIMGHEVA GEVVEVGPGV EDLQVGDYIS VETHIVCGKC YACKHNRYHV CQNTKIFGVD MDGVFAHYAI VPAKNAWKNP KDMPPEYAAL QEPLGNAVDT VLAGPIAGRS TLITGAGPLG LLGIAVAKAS GAYPVIVSEP SEFRRKLAKK VGADYVVNPF EEDPVKFVMD ITDGAGVEVF LEFSGAPKAL EQGLKAVTPG GRVSLLGLFP REVTIDFNNL IIFKALEVHG ITGRHLWETW YTVSSLIQSG KLNLDPIITH KYKGFDKFEE AFELMRAGKT GKVVFFPHKG
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Catalyzes the NAD(+)-dependent oxidation of L-threonine to 2-amino-3-ketobutyrate. To a lesser extent, also catalyzes the oxidation of L-serine.
基因功能參考文獻：
1. Crystallization and preliminary structural studies of L-threonine dehydrogenase from hyperthermophilic archaeon T. kodakaraensis KOD1 are reported and will further our understanding of the enzyme's stability at extremes of temperature and pH. PMID: 18765916
2. TDH expressed in E coli, purified, characterized. Sequence aligned. Activity highest at pH 12, 90C; dependent on divalent cations (e.g., Zn). At 50C, Km is 1.6mM for L-threonine and 0.028mM for NAD+. Half-life is > 2h at 85C, 24min in boiling water. PMID: 19307254
亞細胞定位：

Cytoplasm.
蛋白家族：

Zinc-containing alcohol dehydrogenase family
數據庫鏈接：

KEGG: tko:TK0916

STRING: 69014.TK0916