Recombinant Pseudomonas aeruginosa LexA repressor (lexA)
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中文名稱：銅綠假單胞菌lexA重組蛋白
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貨號：CSB-EP339819EZX-B
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說明書：
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規格：
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來源：E.coli
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共軛：Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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其他：
產品詳情
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純度：>85% (SDS-PAGE)
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基因名：lexA
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Uniprot No.：
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別名：lexA; PA3007LexA repressor; EC 3.4.21.88
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種屬：Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
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蛋白長度：full length protein
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表達區域：1-204
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氨基酸序列MQKLTPRQAE ILSFIKRCLE DHGFPPTRAE IAQELGFKSP NAAEEHLKAL ARKGAIEMTP GASRGIRIPG FEPHAANDDE GLPVIGRVAA GAPILAEQNI EESCRINPAF FNPRADYLLR VRGMSMKDIG ILDGDLLAVH VTREARNGQV VVARIGEEVT VKRFKREGSK VWLLAENPEF APIEVDLKEQ ELIIEGLSVG VIRR
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蛋白標簽：Tag?type?will?be?determined?during?the?manufacturing?process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially. -
產品提供形式：Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
復溶：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
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儲存條件：Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質期：The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期：Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
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注意事項：Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Datasheet ：Please contact us to get it.
產品評價
靶點詳情
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功能：Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair.
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基因功能參考文獻：
- studies shed light on the active site architecture of the LexA autoprotease and provide insights that may inform the design of probes of the SOS pathway. PMID: 24779472
- The LexA-controlled SOS regulon includes only 15 genes but does include several genes that encode proteins involved in recombination and replication, including two polymerases known to play a role in mutation and the evolution of antibiotic resistance PMID: 17015649
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蛋白家族：Peptidase S24 family
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數據庫鏈接：
KEGG: pae:PA3007
STRING: 208964.PA3007
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