Recombinant Porcine epidemic diarrhea virus Replicase polyprotein 1a (1a), partial

中文名稱：

Recombinant Porcine epidemic diarrhea virus Replicase polyprotein 1a (1a),partial
貨號：

CSB-YP314473PPW
說明書：

MSDS Datasheet
規(guī)格：
來源：

Yeast
其他：

中文名稱：

Recombinant Porcine epidemic diarrhea virus Replicase polyprotein 1a (1a),partial
貨號：

CSB-EP314473PPW
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
其他：

中文名稱：

Recombinant Porcine epidemic diarrhea virus Replicase polyprotein 1a (1a),partial
貨號：

CSB-EP314473PPW-B
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Porcine epidemic diarrhea virus Replicase polyprotein 1a (1a),partial
貨號：

CSB-BP314473PPW
說明書：

MSDS Datasheet
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Porcine epidemic diarrhea virus Replicase polyprotein 1a (1a),partial
貨號：

CSB-MP314473PPW
說明書：

MSDS Datasheet
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價
靶點詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

1a
Uniprot No.：

P0C6V6
別名：

1a; Replicase polyprotein 1a; pp1a; ORF1a polyprotein) [Cleaved into: Non-structural protein 1; nsp1; p9); Non-structural protein 2; nsp2; p87); Non-structural protein 3; nsp3; EC 3.4.19.12; EC 3.4.22.-; PL1-PRO/PL2-PRO; PLP1/PLP2; Papain-like proteinases 1/2; p195); Non-structural protein 4; nsp4; Peptide HD2); 3C-like proteinase; 3CL-PRO; 3CLp; EC 3.4.22.-; M-PRO; nsp5; p34); Non-structural protein 6; nsp6); Non-structural protein 7; nsp7; p5); Non-structural protein 8; nsp8; p23); Non-structural protein 9; nsp9; p12); Non-structural protein 10; nsp10; Growth factor-like peptide; GFL; p14); Non-structural protein 11; nsp11)]
種屬：

Porcine epidemic diarrhea virus (strain CV777) (PEDV)
蛋白長度：

Partial
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

The non-structural protein 1 (nsp1) protein plays a role in the inhibition of host interferon and pro-inflammatory cytokines production. Suppresses host RELA/p65 activation by blocking NFKBIA phosphorylation. Targets also the RLR pathway downstream of the IRF3 activation by targeting host CREBBP to proteasomal degradation.; The papain-like proteinase 1 (PLP1) and papain-like proteinase 2 (PLP2) are responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. PLP2 also antagonizes innate immune induction of type I interferon by blocking the nuclear translocation of host IRF-3.; Responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK. Also contains an ADP-ribose-1''-phosphate (ADRP)-binding function.; Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter.; Nsp9 is a ssRNA-binding protein.
亞細(xì)胞定位：

[Non-structural protein 1]: Host cytoplasm. Host nucleus.; [Non-structural protein 3]: Host membrane; Multi-pass membrane protein.; [Non-structural protein 4]: Host membrane; Multi-pass membrane protein.; [Non-structural protein 6]: Host membrane; Multi-pass membrane protein.; [Non-structural protein 7]: Host cytoplasm, host perinuclear region.; [Non-structural protein 8]: Host cytoplasm, host perinuclear region.; [Non-structural protein 9]: Host cytoplasm, host perinuclear region.; [Non-structural protein 10]: Host cytoplasm, host perinuclear region.
蛋白家族：

Coronaviruses polyprotein 1ab family