Recombinant Mycobacterium tuberculosis Immunogenic protein MPT64 (mpt64)

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中文名稱：

結核分枝桿菌mpt64重組蛋白
貨號：

CSB-YP358713FSG
說明書：

MSDS Datasheet
規格：

￥2616
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP358713MVZ could indicate that this peptide derived from Yeast-expressed Mycobacterium tuberculosis mpt64.

Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP358713MVZ could indicate that this peptide derived from Yeast-expressed Mycobacterium tuberculosis mpt64.
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產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

mpt64
Uniprot No.：

P9WIN9
別名：

mpt64; MT2032; Immunogenic protein MPT64; Antigen MPT64
種屬：

Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
蛋白長度：

Full Length of Mature Protein
來源：

Yeast
分子量：

24.4kDa
表達區域：

24-228aa
氨基酸序列

APKTYCEELKGTDTGQACQIQMSDPAYNINISLPSYYPDQKSLENYIAQTRDKFLSAATSSTPREAPYELNITSATYQSAIPPRGTQAVVLKVYQNAGGTHPTTTYKAFDWDQAYRKPITYDTLWQADTDPLPVVFPIVQGELSKQTGQQVSIAPNAGLDPVNYQNFAVTNDGVIFFFNPGELLPEAAGPTQVLVPRSAIDSMLA
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產品描述：

The cDNA fragment encoding 24-228aa of Mycobacterium tuberculosis immunogenic protein MPT64 was fused with an N-terminal 6xHis-tag and then expressed in the yeast cells. The product obtained is the recombinant full-length of mature Mycobacterium tuberculosis MPT64 protein. Its purity was determined using SDS-PAGE and reached up to 90%. Under the reducing conditions, the gel presented a molecular mass band of about 21-24 kDa. It also underwent the validation through the LC-MS/MS analysis. In-stock MPT64 proteins are offered now. This recombinant MPT64 protein may find uses in the specific antibody production or the studies of tuberculosis (TB) infection.

The level of expression of the MPT64 protein is high in patients with active TB. Both in sputum and serum exosomes of a TB infected patient can be detected the presence of MPT64. A special receptor, single-stranded DNA (ssDNA) aptamer, can specifically recognize MPT64 protein. MPT64 mainly promotes survival and persistence in the host cell. It deactivates the expression of apoptotic cytokines and plays a major role in the survival and virulence of the mycobacteria.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

基因功能參考文獻：
1. Aptamers against Mtb secreted protein MPT64 were successfully selected and could be used as an alternative for detection and diagnosis of tuberculosis. PMID: 28454652
2. we found that polymorphisms of the mpt64 gene in the MTBC may be the reason for changes in the antigen produced, which may in turn cause alterations of related functions, thereby allowing immune evasion. PMID: 23390287
3. CFP21 (cutinase precursor) and MPT64 fusion protein stimulate higher level of interferon (IFN)-gamma in tuberculin skin test (TST)-positive healthy population than in TST-negative healthy population. PMID: 21340709
4. Data show that Recombinant Mycobacterium vaccae secreted MPT64 could induce high level humoral and cell mediated immune responses in mice and could be used as a candidate of new vaccine against TB. PMID: 19257983
5. Recombinant rMPT64 elicits a specific immune response indistinguishable from that of MPB64 purified from BCG Tokyo culture filtrates. PMID: 16216527
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亞細胞定位：

Secreted.
數據庫鏈接：

KEGG: mtu:Rv1980c

STRING: 83332.Rv1980c