Recombinant Mouse Vacuolar protein sorting-associated protein 33A (Vps33a)

中文名稱：

小鼠Vps33a重組蛋白
貨號：

CSB-YP871968MO
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

小鼠Vps33a重組蛋白
貨號：

CSB-EP871968MO
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

小鼠Vps33a重組蛋白
貨號：

CSB-EP871968MO-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Vps33a重組蛋白
貨號：

CSB-BP871968MO
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Vps33a重組蛋白
貨號：

CSB-MP871968MO
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Vps33a
Uniprot No.：

Q9D2N9
別名：

Vps33a; Bf; BuffVacuolar protein sorting-associated protein 33A
種屬：

Mus musculus (Mouse)
蛋白長度：

full length protein
表達區域：

1-598
氨基酸序列

MAAHLSYGRV NLNVLREAVR RELREFLDKC AGSKAIVWDE YLTGPFGLIA QYSLLKEHEV EKMFTLKGSR LPAADVKNII FLVRPRLELM DIIAENVLSE DRRGPTRDFH ILFVPRRSLL CEQRLKDLGV LGSFIHREEY SLDLIPFDGD LLSMESEGAF KECYLEGDQT SLYHAAKGLM TLQALYGTIP QIFGKGECAR QVANMMVRMK REFTGSQNSV FPVFDNLLLL DRNVDLLTPL ASQLTYEGLI DEIYGIQNSY VKLPPEKFAP KKQGGGSGGK DLPTEAKKLQ LNSAEELYAE IRDKNFNAVG SVLSKKAKII SAAFEERHNA KTVGEIKQFV SQLPHMQAAR GSLANHTSIA ELIKDVTTSE DFFDKLTVEQ EFMSGIDTDK VNSYIEDCIA QKHPLIKVLR LVCLQSVCNS GLKQKVLDYY RREILQTYGY EHILTLNNLE KAGLLKAQTG GRNNYPTIRK TLRLWMDDVN EQNPTDISYV YSGYAPLSVR LAQLLSRPGW RSIEEVLRIL PGPHFEERQP LPTGLQKKRQ PGENRVTLVF FLGGVTFAEI AALRFLSQLE DGGTEYVIAT TKLMNGNSWI EALMEKPF
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations. Required for fusion of endosomes and autophagosomes with lysosomes; the function is dependent on its association with VPS16 but not VIPAS39. The function in autophagosome-lysosome fusion implicates STX17 but not UVRAG.
基因功能參考文獻：
1. VPS33A(D251E) mutation plays dual roles by increasing the HOPS complex assembly and its association with the autophagic SNARE complex, which selectively affects the autophagosome-lysosome fusion. PMID: 26259518
2. VPS33A is mutated in Hermansky-Pudlak syndrome and may have a role in melanogenesis PMID: 12538872
3. These studies suggest that loss of Purkinje neurons is the most obvious neurological atrophy in the buff mutant mouse, a structural change that generates motor coordination deficits and impaired postural phenotypes. PMID: 19254700
4. Knockdown of Vps33a expression reduced the lysosomal storage of RANKL and caused the accumulation of newly synthesized RANKL in the Golgi apparatus, indicating that Vps33a is involved in transporting RANKL from the Golgi apparatus to secretory lysosomes. PMID: 19419298
相關疾病：

Defects in Vps33a are the cause of the buff mutant which exhibits hypopigmentation in the coat and eyes, due to reduced size and number of melanosomes in their cells. In addition, mice are prone to prolonged bleeding, due to a platelet-storage pool defect.
亞細胞定位：

Cytoplasmic vesicle. Late endosome membrane; Peripheral membrane protein; Cytoplasmic side. Lysosome membrane; Peripheral membrane protein; Cytoplasmic side. Early endosome. Cytoplasmic vesicle, autophagosome. Cytoplasmic vesicle, clathrin-coated vesicle.
蛋白家族：

STXBP/unc-18/SEC1 family
數據庫鏈接：

KEGG: mmu:77573

STRING: 10090.ENSMUSP00000031388

UniGene: Mm.41372