Recombinant Mouse Tripartite motif-containing protein 71 (Trim71), partial

中文名稱：

小鼠Trim71重組蛋白
貨號：

CSB-YP631973MO
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

小鼠Trim71重組蛋白
貨號：

CSB-EP631973MO
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

小鼠Trim71重組蛋白
貨號：

CSB-EP631973MO-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Trim71重組蛋白
貨號：

CSB-BP631973MO
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Trim71重組蛋白
貨號：

CSB-MP631973MO
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Trim71
Uniprot No.：

Q1PSW8
別名：

Trim71; Gm1127; Lin41E3 ubiquitin-protein ligase TRIM71; EC 2.3.2.27; Protein lin-41 homolog; mLin41; RING-type E3 ubiquitin transferase TRIM71; Tripartite motif-containing protein 71
種屬：

Mus musculus (Mouse)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

E3 ubiquitin-protein ligase that cooperates with the microRNAs (miRNAs) machinery and promotes embryonic stem cells proliferation and maintenance. Binds to miRNAs and associates with AGO2, participating in post-transcriptional repression of transcripts such as CDKN1A. Facilitates the G1-S transition to promote rapid embryonic stem cell self-renewal by repressing CDKN1A expression. In addition, participates in post-transcriptional mRNA repression in a miRNA independent mechanism. Required to maintain proliferation and prevent premature differentiation of neural progenitor cells during early neural development: positively regulates FGF signaling by controlling the stability of SHCBP1. Specific regulator of miRNA biogenesis. miRNA Binds MIR29A hairpin and postranscriptionally modulates MIR29A levels, which indirectly regulates TET proteins expression.
基因功能參考文獻：
1. LIN41 interacts with p53, controls its abundance by ubiquitination and antagonizes p53-dependent pro-apoptotic and pro-differentiation responses. In vivo, lack of LIN41 is associated with upregulation of Grhl3 and widespread caspase-3 activation, 2 downstream effectors of p53 with essential roles in neural tube closure. PMID: 28430184
2. suggest that Trim71 keeps priming steps of differentiation in check, which do not pre-require a loss of the pluripotency network in ES cells PMID: 26057209
3. mLin41 acts as a temporal regulator to promote neural progenitor cell maintenance, not via the regulation of AGO2 stability, but through FGF signaling. PMID: 22508726
4. The transcripts of mlin-41 and the protein level in mouse adult tissues and embryos from 9.5 to 13 days were detected by RT-PCR and western blot. PMID: 20824311
5. Lin-41 is genetically and biochemically downstream of both the Shh and Fgf signaling pathways and is expressed in three phases over developmental time and most notably is associated with the developing autopod. PMID: 16245339
6. lin-41 is temporally regulated by miRNAs in order to direct key developmental events such as limb formation. PMID: 16247770
7. Strong loss of function Mlin41 gene-trap mutants demonstrated a striking neural tube closure defect during development, and embryonic lethality. PMID: 19098426
8. demonstrate the presence of mLin41 protein in several stem cell niches, including the embryonic ectoderm, epidermis and male germ line. PMID: 19898466
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亞細胞定位：

Cytoplasm, P-body.
蛋白家族：

TRIM/RBCC family
組織特異性：

Highly expressed in undifferentiated embryonic stem cells (ESCs). Expressed in the epiblast and in interfollicular epidermal stem cells during early development. Also expressed in male germ cells and in the reproductive tract. Highly expressed in neuroepi
數據庫鏈接：

KEGG: mmu:636931

STRING: 10090.ENSMUSP00000107447

UniGene: Mm.17857