Recombinant Mouse Synembryn-A (Ric8a)

中文名稱：

小鼠Ric8a重組蛋白
貨號：

CSB-YP662307MO
說明書：

MSDS Datasheet
規(guī)格：
來源：

Yeast
其他：

中文名稱：

小鼠Ric8a重組蛋白
貨號：

CSB-EP662307MO
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
其他：

中文名稱：

小鼠Ric8a重組蛋白
貨號：

CSB-EP662307MO-B
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Ric8a重組蛋白
貨號：

CSB-BP662307MO
說明書：

MSDS Datasheet
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Ric8a重組蛋白
貨號：

CSB-MP662307MO
說明書：

MSDS Datasheet
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價
靶點詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

Ric8a
Uniprot No.：

Q3TIR3
別名：

Ric8a; Ric8; Synembryn-A; Protein Ric-8A
種屬：

Mus musculus (Mouse)
蛋白長度：

full length protein
表達區(qū)域：

1-530
氨基酸序列

MEPRAVADAL ETGEEDAVTE ALRSFNREHS QSFTFDDAQQ EDRKRLAKLL VSVLEQGLSP KHRVTWLQTI RILSRDRSCL DSFASRQSLH ALACYADITV SEEPIPQSPD MDVLLESLKC LCNLVLSSPT AQMLAAEARL VVRLAERVGL YRKRSYPHEV QFFDLRLLFL LTALRTDVRQ QLFQELHGVR LLTDALELTL GVAPKENPPV MLPAQETERA MEILKVLFNI TFDSVKREVD EEDAALYRYL GTLLRHCVMV EAAGDRTEEF HGHTVNLLGN LPLKCLDVLL ALELHEGSLE FMGVNMDVIS ALLAFLEKRL HQTHRLKECV APVLNVLTEC ARMHRPARKF LKAQVLPPLR DVRTRPEVGD LLRNKLVRLM THLDTDVKRV AAEFLFVLCS ESVPRFIKYT GYGNAAGLLA ARGLMAGGRP EGQYSEDEDT DTEEYREAKA SINPVTGRVE EKPPNPMEGM TEEQKEHEAM KLVNMFDKLS RHRVIQPMGM SPRGHLTSLQ DAMCETMEGQ LSSDPDSDPD
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex. Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation.
基因功能參考文獻：
1. In conditional Ric8a knockout mice the absence of RIC8A severely affected the attachment and positioning of radial glial processes, Cajal-Retzius' cells, and the arachnoid trabeculae, and these mice displayed additional defects in the lens, skeletal muscles, and heart development. All the discovered defects might be linked to aberrancies in cell adhesion and migration. PMID: 29380551
2. found that RIC8A plays an important role in the organisation and remodelling of actin cytoskeleton and cell-extracellular matrix association PMID: 28526238
3. Results suggest that RIC8A has an essential role in the development of mammalian nervous system by maintaining the integrity of pial basement membrane and modulating cell division PMID: 25641781
4. Data show that the localization of maternally expressed RIC8 protein is highly dynamic and is dependent on the stage of folliculogenesis, oogenesis and cleavage, and imply that it may have a regulatory function in mammalian gametogenesis. PMID: 26062014
5. Data show that disrupting resistance to inhibitors of cholinesterase 8A (Ric-8A) expression in hematopoietic cells results in a loss of GTP-binding protein alpha subunits Galphai2, Galphai3, and Galphaq. PMID: 26232433
6. The activity of RIC8A in neurons is essential for survival and its deficiency causes a severe neuromuscular phenotype. PMID: 23977396
7. Ric-8 proteins support G protein levels by serving as molecular chaperones that promote Galpha subunit biosynthesis PMID: 23384070
8. These data indicate a dynamic interaction between GPR proteins, Galpha(i1) and Ric-8A, in the cell that influences subcellular localization of the three proteins and regulates complex formation. PMID: 23212907
9. The results of this study revealed a novel role of Ric-8a in modulating Bergmann glia-basement membrane adhesion during foliation. PMID: 23100420
10. NCAM180 regulates Ric8A membrane localization and potentiates beta-adrenergic response PMID: 22384181
11. data suggest that Ric-8 proteins are molecular chaperones required for the initial association of nascent Galpha subunits with cellular membranes PMID: 22114146
12. Ric-8A is critical for growth factor receptor-induced actin cytoskeletal reorganization PMID: 21771786
13. Activation of the Rsg14-Galphai1-GDP signaling complex is regulated by Ric8. PMID: 21158412
14. Study reveals that the activity of RIC-8 protein is irreplaceable for the correct gastrulation of mouse embryo. PMID: 21069829
15. Here we show that during the early development in mice (E9.5-E12.0) ric-8 (synembryn) is expressed in the developing nervous system PMID: 12971991
16. Based on these results we can conclude the importance of ric-8 in the regulation of memory and emotional behavior. PMID: 16221497
17. These results suggested that Ric-8A potentiates Gq-mediated signal transduction by acting as a novel-type regulator in intact cells. PMID: 16629901
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亞細胞定位：

Cytoplasm. Cell membrane.
蛋白家族：

Synembryn family
組織特異性：

Expressed in neurons and neurites of the CA1 and CA2 subregions of the hippocampus (at protein level). In adult brain, it is expressed in the neocortex, hippocampus and cerebellum as well as in the pineal gland and ependymal layer.
數(shù)據(jù)庫鏈接：

KEGG: mmu:101489

STRING: 10090.ENSMUSP00000026558

UniGene: Mm.281916