Recombinant Mouse Sperm flagellar protein 1 (Spef1)

中文名稱：

小鼠Spef1重組蛋白
貨號：

CSB-YP859538MO
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

小鼠Spef1重組蛋白
貨號：

CSB-EP859538MO
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

小鼠Spef1重組蛋白
貨號：

CSB-EP859538MO-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Spef1重組蛋白
貨號：

CSB-BP859538MO
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Spef1重組蛋白
貨號：

CSB-MP859538MO
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Spef1
Uniprot No.：

Q99JL1
別名：

Spef1; Clamp; Sperm flagellar protein 1; Calponin-homology and microtubule-associated protein
種屬：

Mus musculus (Mouse)
蛋白長度：

full length protein
表達區域：

1-234
氨基酸序列

MESSVDEEAL HQLYLWVDNI PLSRPKRNLS RDFSDGVLVA ELIKFYFPKM VEMHNYVPAN SLQQKLSNWG HLNRKVLNKL NFSVPDDVMR KIAQCSPGVV ELVLIPLRQR LEERQRRQKL GVGSLQELAP QDSSGYMDMG LPQKVRGEGA PALGEQLREG RPLASRPPGY NQALQGDPSF VLQIAEKEQE LLASQETVQV LQMKVKRLEH LLQLKNVRID DLSRRLQQAE RKQR
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Microtubule-associated protein that promotes microtubule bundling and stabilizes microtubules against depolymerization in response to cold shock. Microtubule-associated protein involved in the stabilization of microtubules along the axis of migration during radial intercalation. Promotes the establishment and stabilization of an axis of microtubules required for the active migration of cells into the outer epithelium. Essential for ciliary central apparatus formation which requires both its microtubule-binding and bundling activities and for ciliary localization of HYDIN and SPAG6 in ependymal cilia. Binds actin in intestinal epithelial cells (IECs), essential for IECs survival and contributes to formation of filopodia and lamellipodia in migrating IECs. Regulates planar cell polarity signaling pathway and asymmetric microtubule accumulation in ciliated epithelia.
基因功能參考文獻：
1. demonstrate a specific pattern of Spef1 localisation in the seminiferous epithelium of adult mouse testis PMID: 15979255
2. a.k.a. Spef1 (sperm flagella) and CLAMP (CaLponin-homology And Microtubule associated Protein) CLAMP/Spef1 expression also in brain, lung, and inner ear MT association of CLAMP requires the CH domain, which has highest similarity with EB family proteins PMID: 16206169
亞細胞定位：

Cytoplasm. Cell projection, cilium, flagellum. Cytoplasm, cytoskeleton, cilium axoneme. Apical cell membrane. Basolateral cell membrane. Cytoplasm, cytoskeleton, stress fiber. Cell projection, microvillus. Cell projection, lamellipodium. Cell projection, filopodium.
組織特異性：

Expressed predominantly in the seminiferous epithelium of adult testis. Expressed in pillar cells of the organ of Corti (at protein level). Expressed in brain, kidney, lung and testis. Highly expressed in the trachea, lung and oviduct.
數據庫鏈接：

KEGG: mmu:70997

STRING: 10090.ENSMUSP00000028801

UniGene: Mm.252722