Recombinant Mouse Regenerating islet-derived protein 3-gamma (Reg3g), partial

^{In
Stock}

中文名稱：

小鼠Reg3g重組蛋白
貨號：

CSB-YP019549MO
說明書：

MSDS Datasheet
規格：

￥2208
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

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靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

Reg3g
Uniprot No.：

O09049
別名：

Reg3g; Pap3; Regenerating islet-derived protein 3-gamma; REG-3-gamma; Pancreatitis-associated protein 3; Regenerating islet-derived protein III-gamma; Reg III-gamma) [Cleaved into: Regenerating islet-derived protein 3-gamma 16.5 kDa form; Regenerating islet-derived protein 3-gamma 15 kDa form]
種屬：

Mus musculus (Mouse)
蛋白長度：

Partial
來源：

Yeast
分子量：

18.3kDa
表達區域：

27-174aa
氨基酸序列

EVAKKDAPSSRSSCPKGSRAYGSYCYALFSVSKNWYDADMACQKRPSGHLVSVLSGAEASFLSSMIKSSGNSGQYVWIGLHDPTLGYEPNRGGWEWSNADVMNYINWETNPSSSSGNHCGTLSRASGFLKWRENYCNLELPYVCKFKA
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

問答及客戶評論

■ 常見問題解答

I have another protein question for you.I would like to know if it is necessary to remove the His tag from this protein prior to their experiment.Is there a standard protocol for tag removal that your laboratory would recommend?

Thanks for your inquiry.
1. This protein is N-terminal 6xHis-tagged. This tag is very small which will not affect your experiment in theory. If your want to remove the tag, they could try, and we have successfully remove the tag from this protein before.
2. Thrombin cleavage site has been added between the tag and the target protein when this protein was designed, you can choose thrombin for cleavage.
3. You could purchase thrombin and remove the tag according to product operation instructions.

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靶點詳情

功能：

Bactericidal C-type lectin which acts exclusively against Gram-positive bacteria and mediates bacterial killing by binding to surface-exposed carbohydrate moieties of peptidoglycan. Restricts bacterial colonization of the intestinal epithelial surface and consequently limits activation of adaptive immune responses by the microbiota. The uncleaved form has bacteriostatic activity, whereas the cleaved form has bactericidal activity against L.monocytogenes and methicillin-resistant S.aureus. Regulates keratinocyte proliferation and differentiation after skin injury.
基因功能參考文獻：
1. the secretion of regenerating islet-derived IIIgamma (RegIIIgamma) is responsible for the protective effect of Adipose-derived stem cells. PMID: 26866937
2. The study shows that cardiac stress activates Reg3gamma expression and p38 MAPK is an upstream regulator of Reg3gamma gene expression in heart. Altogether the data suggest Reg3gamma is associated with cardiac inflammatory signaling. PMID: 27798352
3. regenerating islet-derived gene gamma promotes beta cell regeneration and preserves beta cells from autoimmunity damage by increasing regulatory T cell differentiation and inducing tolerated dendritic cells. PMID: 26667474
4. Recombinant IFN-I or IFN-I expression induced by RIG-I promoted growth of intestinal organoids in vitro and production of the antimicrobial peptide regenerating islet-derived protein 3 gamma (RegIIIgamma). PMID: 28424327
5. REG3gamma-deficient mice have altered mucus distribution and increased mucosal inflammatory responses to the microbiota and enteric pathogens in the ileum. PMID: 24345802
6. antibacterial function for lung epithelium through Stat3-mediated induction of Reg3gamma. PMID: 23401489
7. Reg-IIIgamma expression is mainly observed in epithelial cells of the airways and intestine, but not in the nervous system; expression levels show a gradually increasing pattern along with development. PMID: 21681751
8. Letter: aggregation status of PAP1 and PAP2 could change their role in regulating the inflammation in acute pancreatitis. PMID: 21926556
9. findings show that RegIIIgamma is essential for maintaining a ~50-micrometer zone that physically separates the microbiota from the small intestinal epithelial surface PMID: 21998396
10. findings show that RegIIIgamma and its human counterpart, HIP/PAP, are directly antimicrobial proteins that bind their bacterial targets via interactions with peptidoglycan carbohydrate PMID: 16931762
11. antibacterial activities of mouse RegIIIgamma and its human ortholog, HIP/PAP, are tightly controlled by an inhibitory N-terminal prosegment that is removed by trypsin in vivo. PMID: 19095652
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亞細胞定位：

Secreted. Cytoplasm.
組織特異性：

Predominantly expressed in the small intestine, including Paneth cells (at protein level). Hardly detectable in the colon (at protein level). Highly expressed in the lung epithelium during methicillin-resistant S.aureus infection (at protein level). Skin
數據庫鏈接：

KEGG: mmu:19695

STRING: 10090.ENSMUSP00000032089

UniGene: Mm.252385