Recombinant Mouse Protein Filia

中文名稱：

小鼠Khdc3重組蛋白
貨號：

CSB-YP887455MO
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

小鼠Khdc3重組蛋白
貨號：

CSB-EP887455MO
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

小鼠Khdc3重組蛋白
貨號：

CSB-EP887455MO-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Khdc3重組蛋白
貨號：

CSB-BP887455MO
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Khdc3重組蛋白
貨號：

CSB-MP887455MO
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Khdc3
Uniprot No.：

Q9CWU5
別名：

Khdc3KH domain-containing protein 3; Protein Filia
種屬：

Mus musculus (Mouse)
蛋白長度：

full length protein
表達區域：

1-440
氨基酸序列

MASLKRFQTL VPLDHKQGTL FEIIGEPKLP KWFHVECLED PKRLYVEPRL LEIMFGKDGE HIPHLESMLH TLIHVNVWGP ERRAEIWIFG PPPFRRDVDR MLTDLAHYCR MKLMEIEALE AGVERRRMAA HKAATQPAPV KVREAAPRPA SVKVPETATQ PAPVKVREAA PQPAPVQEVR EAAPQQASVQ EEVREAATEQ APVQEVREAA TEQAPVQEVS EAATEQAPVQ EVNEAATEQA SVQAVREAAT RPAPGKVRKA ATQPAPVQVC QEATQLAPVK VREAATQPAS GKVREAATQL APVKVRKAAT QLAPVKVHEA ATQPAPGKVS DAATQSASVQ VREAATQLSP VEATDTSQLA QVKADEAFAQ HTSGEAHQVA NGQSPIEVCE TATGQHSLDV SRALSQKCPE VFEWETQSCL DGSYVIVQPP RDAWESFIIL
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

As part of the OOEP-KHDC3 scaffold, recruits BLM and TRIM25 to DNA replication forks, thereby promoting the ubiquitination of BLM by TRIM25, enhancing BLM retainment at replication forks and therefore promoting stalled replication fork restart. Regulates homologous recombination-mediated DNA repair via recruitment of RAD51 to sites of DNA double-strand breaks, and sustainment of PARP1 activity, which in turn modulates downstream ATM or ATR activation. Activation of ATM or ATR in response to DNA double-strand breaks may be cell-type specific. Its role in DNA double-strand break repair is independent of its role in restarting stalled replication forks. As a member of the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics. Required for maintenance of euploidy during cleavage-stage embryogenesis. Required for the formation of F-actin cytoplasmic lattices in oocytes which in turn are responsible for symmetric division of zygotes via the regulation of mitotic spindle formation and positioning. Ensures proper spindle assembly by regulating the localization of AURKA via RHOA signaling and of PLK1 via a RHOA-independent process. Required for the localization of MAD2L1 to kinetochores to enable spindle assembly checkpoint function. Promotes neural stem cell neurogenesis and neuronal differentiation in the hippocampus. May regulate normal development of learning, memory and anxiety. Capable of binding RNA.
基因功能參考文獻：
1. the identification of Filia as a specific regulator of genomic stability in mouse embryonic stem cells, is reported. PMID: 25936915
2. FILIA is part of the subcortical maternal complex (SCMC). SCMC is required for formation of the cytoplasmic F-actin meshwork that controls the central position of the spindle and ensures symmetric division. PMID: 25208553
3. FILIA and FILIA-N could sequester specific RNA components, supporting the critical roles of FILIA in regulation of RNA transcripts during mouse oogenesis and early embryogenesis. PMID: 22276159
4. High-quality diffraction data were collected from the two crystal forms of FILIA to resolutions of 1.8 and 2.2 A, respectively, using synchrotron radiation PMID: 20823540
5. Filia is central to integrating the spatiotemporal localization of regulators that helps ensure euploidy and high-quality cell cycle progression in preimplantation mouse development. PMID: 19376971
6. This paper states that the ECAT1 gene is absent in rodents. PMID: 17913455
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亞細胞定位：

Cytoplasm, cell cortex. Nucleus. Mitochondrion. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome.
蛋白家族：

KHDC1 family
組織特異性：

Detected in ovary, but not in testis or somatic tissues. In the ovary, expressed in growing oocytes.
數據庫鏈接：

KEGG: mmu:66991

STRING: 10090.ENSMUSP00000034737

UniGene: Mm.471745