Recombinant Mouse Prolyl 3-hydroxylase 3 (Leprel2)

中文名稱：

小鼠P3h3重組蛋白
貨號：

CSB-YP804962MO
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

小鼠P3h3重組蛋白
貨號：

CSB-EP804962MO
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

小鼠P3h3重組蛋白
貨號：

CSB-EP804962MO-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠P3h3重組蛋白
貨號：

CSB-BP804962MO
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

小鼠P3h3重組蛋白
貨號：

CSB-MP804962MO
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

P3h3
Uniprot No.：

Q8CG70
別名：

P3h3; Leprel2; Prolyl 3-hydroxylase 3; EC 1.14.11.7; Leprecan-like protein 2; Protein B
種屬：

Mus musculus (Mouse)
蛋白長度：

Full Length of Mature Protein
表達區域：

20-732
氨基酸序列

E PPEPPGLAQL SPGSPPQAPD LLYADGLRAY SAGAWAPAVA LLREALRSRA ALGRARQECG ASCAAEPGAA LPSQLLGAPH PVSGPGVWEP LLLRATLRRA ECLTQCAVRR LGPGGAARLR VGSALRDAFR RREPYNYLQR AYYQLKKLDL AASAAHTFFV ANPTHLQMRE DMAKYRRMSA IRPQSFRDLV TPLYWAAYDT GLELLEQREA ALALPQLEEA LQGSLAHMES CRAACEGPEE HQGAEEEGEG SQGGLYEAIA GHWIRVLQCR QHCVADTATR PGRSFPVQDF LLSQLRRLHE AYAQVGNMSQ AMENVLSVLL FYPEDEAAKK ALNQYQTQLG EPRPDLGPRE DIQRFILRSL GEKRQLYYAM EHLGTSFKDP DSWTPEALIP KALRERLRED QEKKPWDHQP PQQKPLAHWK DALLMEGVTL TQDAQQLNGS ERAVLDGLLT SAECGVLLQL AKDAAQAGAR SGYRGRRSPH SPHERFEGLT VLKAAQLARA GTVGRPGAKL LLEVSERVRT LTQAYFSPER PLHLSFTHLV CRSAIEGEQE QRMDLSHPVH ADNCVLDPDT GECWREPPAY TYRDYSGLLY LNDDFKGGDL FFTQPNALTV TAQVRPRCGR LVAFSSGGEN PHGVWAVTRG RRCALALWHT WAPEHSEQEW TEAKELLQEE EEEEEEEDIL SRDPSPEPPS HKLQRVQEKA GKPRRVRVRE EL
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Part of a complex composed of PLOD1, P3H3 and P3H4 that catalyzes hydroxylation of lysine residues in collagen alpha chains and is required for normal assembly and cross-linkling of collagen fibrils. Required for normal hydroxylation of lysine residues in type I collagen chains in skin, bone, tendon, aorta and cornea. Required for normal skin stability via its role in hydroxylation of lysine residues in collagen alpha chains and in collagen fibril assembly. Apparently not required for normal prolyl 3-hydroxylation on collagen chains, possibly because it functions redundantly with other prolyl 3-hydroxylases.
基因功能參考文獻：
1. both P3h3- and Sc65-null mice displayed a similar pattern of severe under-hydroxylation of lysines, particularly at cross-linking sites in type I collagen from bone, skin, dentin, cornea, and aorta, revealing a complex evolution and interplay between the proteins forming the machinery of collagen prolyl 3-hydroxylation and lysyl hydroxylation in the ER PMID: 28115524
2. Study found that all 3 mouse Leprecan genes Lepre1, leprel1 and leprel2 are expressed in the notochord; additionally, they are expressed in dorsal root ganglia, limb buds, branchial arches, and developing kidneys. PMID: 18798549
3. This report characterizes expression of the P3H1, P3H2 and P3H3 genes in embryonic and adult mice. PMID: 19652424
亞細胞定位：

Endoplasmic reticulum.
蛋白家族：

Leprecan family
組織特異性：

Detected in kidney (at protein level).
數據庫鏈接：

KEGG: mmu:14789

STRING: 10090.ENSMUSP00000023958

UniGene: Mm.35708