Recombinant Mouse Probable tubulin polyglutamylase TTLL1 (Ttll1)

中文名稱：

小鼠Ttll1重組蛋白
貨號(hào)：

CSB-YP856290MO
說明書：

MSDS Datasheet
規(guī)格：
來源：

Yeast
其他：

中文名稱：

小鼠Ttll1重組蛋白
貨號(hào)：

CSB-EP856290MO
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
其他：

中文名稱：

小鼠Ttll1重組蛋白
貨號(hào)：

CSB-EP856290MO-B
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Ttll1重組蛋白
貨號(hào)：

CSB-BP856290MO
說明書：

MSDS Datasheet
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Ttll1重組蛋白
貨號(hào)：

CSB-MP856290MO
說明書：

MSDS Datasheet
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

Ttll1
Uniprot No.：

Q91V51
別名：

Ttll1; Probable tubulin polyglutamylase TTLL1; EC 6.-.-.-; Tubulin polyglutamylase complex subunit 3; PGs3; Tubulin--tyrosine ligase-like protein 1; p49
種屬：

Mus musculus (Mouse)
蛋白長(zhǎng)度：

full length protein
表達(dá)區(qū)域：

1-423
氨基酸序列

MAGRVKWVTD IEKSVLINNF EKRGWIQVTE NEDWNFYWMS VQTIRNVFSV ETGYRLSDDQ IVNHFPNHYE LTRKDLMVKN IKRYRKELEK EGSPLAEKDE NGKYLYLDFV PVTYMLPADY NLFVEEFRKS PSSTWIMKPC GKAQGKGIFL INKLSQIKKW SRDSKTSSFV SQSTKEAYVI SVYINNPLLI GGRKFDLRLY VLVSTYRPLR CYMYKLGFCR FCTVKYTPST SELDNMFVHL TNVAIQKHGE DYNHIHGGKW TVNNLRLYLE STRGREVTSK LFDEIHWIIV QSLKAVAPVM NNDKHCFECY GYDIIIDDKL KPWLIEVNAS PSLTSSTAND RILKYNLIND TLNIAVPNGE IPDCKWNKSP PKEVLGNYEI LYDEELAQGD GAERELRNRP GQPVGPRAGR SRDSGRSVLT TWK
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Catalytic subunit of a polyglutamylase complex which modifies tubulin, generating side chains of glutamate on the gamma-carboxyl group of specific glutamate residues within the C-terminal tail of tubulin. Probably involved in the side-chain elongation step of the polyglutamylation reaction rather than the initiation step. Modifies both alpha- and beta-tubulins with a preference for the alpha-tail. Unlike most polyglutamylases of the tubulin--tyrosine ligase family, only displays a catalytic activity when in complex with other proteins as it is most likely lacking domains important for autonomous activity. Part of the neuronal tubulin polyglutamylase complex. Mediates cilia and flagella polyglutamylation which is essential for their biogenesis and motility. Involved in respiratory motile cilia function through the regulation of beating asymmetry. Essential for sperm flagella biogenesis, motility and male fertility. Also mediates glutamylation of non-tubulin proteins. Involved in KLF4 glutamylation which impedes its ubiquitination, thereby leading to somatic cell reprogramming, pluripotency maintenance and embryogenesis.
基因功能參考文獻(xiàn)：
1. Klf4 polyglutamylation plays a critical role in the regulation of cell reprogramming and pluripotency maintenance in a process that involves TTLL4 and TTLL1 PMID: 29593216
2. TTLL1 plays a role in establishing normal functions of specific cilia and flagella. PMID: 20442420
3. loss of beating asymmetry resulted in a reduction of cilia-generated fluid flow in trachea from the Ttll1 knock-out mice. PMID: 20498047
4. findings showed that the neuronal tubulin polyglutamylase is a protein complex containing a tubulin tyrosine ligase-like (TTLL) protein, TTLL1 PMID: 15890843
亞細(xì)胞定位：

Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, cilium basal body. Cytoplasm, cytoskeleton, cilium axoneme. Cell projection, cilium, flagellum.
蛋白家族：

Tubulin polyglutamylase family
組織特異性：

Highly expressed in brain, heart and kidney. Expressed in liver, lung, muscle, spleen, testis and trachea. In the brain, expressed in ependymal cilia, cortex, corpus callosum and striatum. Expressed in blastomere.
數(shù)據(jù)庫(kù)鏈接：

KEGG: mmu:319953

STRING: 10090.ENSMUSP00000016897

UniGene: Mm.235007