Recombinant Mouse PAX-interacting protein 1 (Paxip1), partial

中文名稱：

小鼠Paxip1重組蛋白
貨號：

CSB-YP761262MO
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

小鼠Paxip1重組蛋白
貨號：

CSB-EP761262MO
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

小鼠Paxip1重組蛋白
貨號：

CSB-EP761262MO-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Paxip1重組蛋白
貨號：

CSB-BP761262MO
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Paxip1重組蛋白
貨號：

CSB-MP761262MO
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Paxip1
Uniprot No.：

Q6NZQ4
別名：

Paxip1; PtipPAX-interacting protein 1; PAX transactivation activation domain-interacting protein
種屬：

Mus musculus (Mouse)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Involved in DNA damage response and in transcriptional regulation through histone methyltransferase (HMT) complexes such as the MLL2/MLL3 complex. Plays a role in early development. In DNA damage response is required for cell survival after ionizing radiation. In vitro shown to be involved in the homologous recombination mechanism for the repair of double-strand breaks (DSBs). Its localization to DNA damage foci requires Rnf8 and Ube2n. Recruits Tp53bp1 to DNA damage foci and, at least in particular repair processes, effective DNA damage response appears to require the association with Tp53bp1 phosphorylated by Atm. Together with Tp53bp1 regulates Atm association. Proposed to recruit Pagr1 to sites of DNA damage and the Pagr1:Paxip1 complex is required for cell survival in response to DNA damage independently of the MLL2/MLL3 complex. However, this function has been questioned. Promotes ubiquitination of PCNA following UV irradiation and may regulate recruitment of polymerase eta and Rad51 to chromatin after DNA damage. Proposed to be involved in transcriptional regulation by linking MLL-containing histone methyltransferase (HMT) complexes to gene promoters by interacting with promoter-bound transcription factors such as Pax2. Associates with gene promoters that are known to be regulated by Kmt2d/Mll2. During immunoglobulin class switching in activated B-cells is involved in trimethylation of histone H3 at 'Lys-4' and in transcription initiation of downstream switch regions at the immunoglobulin heavy-chain (Igh) locus; this function appears to involve the recruitment of MLL-containing HMT complexes. Conflictingly, its function in transcriptional regulation during immunoglobulin class switching is reported to be independent of the MLL2/MLL3 complex.
基因功能參考文獻：
1. PTIP as an epigenetic regulator of osteoclastogenesis. PMID: 30297393
2. The role for PTIP in promoting sterile transcription at the Igh locus. PMID: 29078319
3. a PTIP-PA1 subcomplex functions independently from the MLL3/MLL4 complex to mediate transcription during CSR PMID: 26744420
4. Polyglutamine expansion AR sequesters PTIP to attenuate DNA repair and increase genomic instability. PMID: 22736030
5. PAXIP1 controls distinct transcriptional programs during DP differentiation necessary for Tcra locus accessibility, licensing mature thymocytes for trafficking and natural killer T cell development. PMID: 23159437
6. PTIP plays an important role in the DNA repair process associated with the development of mature spermatozoa. PMID: 23063797
7. PTIP stabilizes the Pax5 DNA interactions that promote chromatin looping and regulate transcriptional responses needed for class switch recombination. PMID: 21282469
8. Data show that loss of PTIP resulting in subtle changes in gene expression patterns and lower H3K4 methylation. PMID: 21060806
9. PTIP accumulation at double stranded breaks contributes to class switch recombination and genome stability independently of Igh switch transcription PMID: 20671152
10. PTIP is an essential element of the cell proliferation machinery, perhaps by functioning in the DNA repair pathways PMID: 12588986
11. Pax transactivation domain-interacting protein is required for the urine concentration mechanism by modulating arginine vasopressin receptor 2 and AQP2 expression in the inner medulla. PMID: 17429055
12. PTIP bridges DNA-binding developmental regulators to histone methyltransferase-dependent epigenetic regulation. PMID: 17925232
13. These and other data suggest that PTIP has multiple functions, one of which is to promote the formation of transcriptional complexes that provide specificity of developmental gene expression. PMID: 18710940
14. subset of PTIP.PA1 complex is recruited to DNA damage sites via the RNF8-dependent pathway and is required for cell survival in response to DNA damage. PMID: 19124460
15. The results suggest that the maintenance of H3K4 methylation is essential and requires PTIP function during the in vitro propagation of pluripotent ES cells. PMID: 19544464
16. The PTIP regulates PPAR gamma and C/EBPalpha expression in mouse embryonic fibroblasts as well as during preadipocyte differentiation. PMID: 19583951
17. Without DNA damage agent treatment, endogenous PTIP associates with MLL3- and MLL4-containing histone H3 K4 methyltransferase complex that also contains ASH2L, RBBP5, WDR5, hDPY-30, NCOA6, PA1 and JmjC domain-containing putative histone demethylase UTX. PMID: 17500065
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亞細胞定位：

Nucleus matrix. Chromosome.
組織特異性：

Expression detected in all tissues examined, including brain stem, cerebellum, cortex, heart, spleen, kidney, liver, thymus and lung.
數據庫鏈接：

KEGG: mmu:55982

STRING: 10090.ENSMUSP00000002291

UniGene: Mm.277190