Recombinant Mouse Non-homologous end-joining factor 1 (Nhej1)

中文名稱：

小鼠Nhej1重組蛋白
貨號：

CSB-YP662047MO
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

小鼠Nhej1重組蛋白
貨號：

CSB-EP662047MO
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

小鼠Nhej1重組蛋白
貨號：

CSB-EP662047MO-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Nhej1重組蛋白
貨號：

CSB-BP662047MO
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Nhej1重組蛋白
貨號：

CSB-MP662047MO
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Nhej1
Uniprot No.：

Q3KNJ2
別名：

Nhej1; Xlf; Non-homologous end-joining factor 1; Protein cernunnos; XRCC4-like factor
種屬：

Mus musculus (Mouse)
蛋白長度：

full length protein
表達區域：

1-295
氨基酸序列

MEELEQDLLL QPWAWLQLAE NSLLAKVSIT KHGYALLISD LQQVWHEQVD TSVVSQRAKE LNKRLTAPPA ALLCHLDEAL RPLFKDSAHP SKATFSCDRG EEGLILRVQS ELSGLPFSWH FHCIPASSSL VSQHLIHPLM GVSLALQSHV RELAALLRMK DLEIQAYQES GAVLSRSRLK TEPFEENSFL EQFMAEKLPE ACAVGDGKPF AMSLQSLYVA VTKQQIQARQ AHKDSGETQA SSSTSPRGTD NQPEEPVSLS STLSEPEYEP VAASGPMHRA RLVKSKRKKP RGLFS
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

DNA repair protein involved in DNA non-homologous end joining (NHEJ); required for double-strand break (DSB) repair and V(D)J recombination. Plays a key role in NHEJ by promoting the ligation of various mismatched and non-cohesive ends. Interacts with POLL (DNA polymerase lambda); promoting POLL recruitment to double-strand breaks (DSBs) and stimulation of the end-filling activity of POLL. May act in concert with XRCC5-XRCC6 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are non-complementary or partially complementary. Associates with XRCC4 to form alternating helical filaments that bridge DNA and act like a bandage, holding together the broken DNA until it is repaired. The XRCC4-NHEJ1/XLF subcomplex binds to the DNA fragments of a DSB in a highly diffusive manner and robustly bridges two independent DNA molecules, holding the broken DNA fragments in close proximity to one other. The mobility of the bridges ensures that the ends remain accessible for further processing by other repair factors. Binds DNA in a length-dependent manner.
基因功能參考文獻：
1. we also find that PAXX deficiency has no impact on V(D)J recombination DSB joining in ATM-deficient pro-B lines. We discuss implications of these findings with respect to potential PAXX and XLF functions in C-NHEJ. PMID: 27601633
2. Ku70 is epistatic with XLF and DNA-PKcs and support a model in which inactivation of Ku70 allows DNA lesions to become accessible to alternative DNA repair pathways (other than Classical Non-Homologous End-Joining (C-NHEJ)). PMID: 28759779
3. XLF has an important role during V(D)J recombination. PMID: 27281794
4. PAXX and XLF proteins may have redundant functions during Non-homologous end joining. PMID: 27830975
5. Paxx/Xlf double-knockout mice display embryonic lethality associated with genomic instability, cell death in the central nervous system, and an almost complete block in lymphogenesis PMID: 27798842
6. These results reveal an unanticipated functional interplay between the RAG complex and XLF in repairing RAG-induced DNA breaks and maintaining genome integrity during antigen receptor gene assembly. PMID: 26833222
7. XLF-deficient mice recapitulate the age-dependent lymphocytopenia of patients. PMID: 25075129
8. XLF functionally overlaps with DNA-PKcs in normal development, promotion of genomic stability in fibroblasts, and in IgH class switch recombination in mature B cells. PMID: 23345432
9. Cernunnos deficiency results in chronic activation of the DNA damage response, P53-driven upregulation of proapoptotic factors, leading to decreased thymocyte viability and a qualitative alteration of the T cell repertoire in both humans and mice. PMID: 23207905
10. XLF repair protein and 53BP1 DNA damage response factor have overlapping functions in end joining and lymphocyte development PMID: 22355127
11. find that combined XLF/53BP1 deficiency in mice severely impairs C-NHEJ, V(D)J recombination, and lymphocyte development while also leading to general genomic instability and growth defects PMID: 22308489
12. XLF, ATM and H2AX all have fundamental roles in processing and joining DNA ends during V(D)J recombination, but that these roles have been masked by unanticipated functional redundancies PMID: 21160472
13. in mice, Cernunnos-XLF is essential for normal NHEJ-mediated repair of DNA DSBs and the Cernunnos-XLF acts as a genomic caretaker to prevent genomic instability PMID: 17360556
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亞細胞定位：

Nucleus. Chromosome.
蛋白家族：

XLF family
數據庫鏈接：

KEGG: mmu:75570

STRING: 10090.ENSMUSP00000116797

UniGene: Mm.442409