Recombinant Mouse Neutral ceramidase (Asah2), partial

中文名稱：

Recombinant Mouse Neutral ceramidase(Asah2),partial
貨號：

CSB-YP881290MO
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

Recombinant Mouse Neutral ceramidase(Asah2),partial
貨號：

CSB-EP881290MO
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

Recombinant Mouse Neutral ceramidase(Asah2),partial
貨號：

CSB-EP881290MO-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Mouse Neutral ceramidase(Asah2),partial
貨號：

CSB-BP881290MO
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Mouse Neutral ceramidase(Asah2),partial
貨號：

CSB-MP881290MO
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Asah2
Uniprot No.：

Q9JHE3
別名：

Asah2Neutral ceramidase; N-CDase; NCDase; EC 3.5.1.-; EC 3.5.1.23; Acylsphingosine deacylase 2; N-acylsphingosine amidohydrolase 2) [Cleaved into: Neutral ceramidase soluble form]
種屬：

Mus musculus (Mouse)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Plasma membrane ceramidase that hydrolyzes sphingolipid ceramides into sphingosine and free fatty acids at neutral pH. Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation. Also catalyzes the reverse reaction allowing the synthesis of ceramides from fatty acids and sphingosine. Together with sphingomyelinase, participates in the production of sphingosine and sphingosine-1-phosphate from the degradation of sphingomyelin, a sphingolipid enriched in the plasma membrane of cells. Also participates in the hydrolysis of ceramides from the extracellular milieu allowing the production of sphingosine-1-phosphate inside and outside cells. This is the case for instance with the digestion of dietary sphingolipids in the intestinal tract.
基因功能參考文獻：
1. These results suggest that neutral ceramidase is a mediator of necroptosis and might be a novel therapeutic target for protection from ischaemic injury. PMID: 26747710
2. Knocking down NCDase reduced sphingosine accumulation in mitochondria and preserved COX activity after the brain injury. PMID: 24659784
3. Neutral ceramidase deficiency increases inflammation in a mouse model of inflammatory bowel disease. PMID: 22940715
4. Novel pathway of ceramide production in mitochondria: thioesterase and neutral ceramidase produce ceramide from sphingosine and acyl-CoA. PMID: 21613224
5. the predicted HNF-4alpha binding site identified in the Asah2 promoter is functional PMID: 20803549
6. molecular cloning of the mouse neutral ceramidase gene and its promoter analysis PMID: 12435403
7. Neutral ceramidase secreted by endothelial cells is released in part associated with caveolin-1. PMID: 12921776
8. the Asah2-encoded neutral ceramidase is a key enzyme for the catabolism of dietary sphingolipids and regulates the levels of bioactive sphingolipid metabolites in the intestinal tract PMID: 16380386
9. This enzyme is decreased in the small intestine in CFTR transgenic mice with a deletion in CFTR> PMID: 18955860
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亞細胞定位：

[Neutral ceramidase]: Cell membrane; Single-pass type II membrane protein. Membrane raft; Single-pass type II membrane protein. Membrane, caveola; Single-pass type II membrane protein. Golgi apparatus membrane; Single-pass type II membrane protein. Mitochondrion. Secreted, extracellular exosome.; [Neutral ceramidase soluble form]: Secreted.
蛋白家族：

Neutral ceramidase family
組織特異性：

Widely expressed. Strongly expressed in small intestine and to a lower extent in liver and kidney. Highly expressed in duodenum, jejunum and ileum along the brush border of the small intestine (at protein level).
數據庫鏈接：

KEGG: mmu:54447

STRING: 10090.ENSMUSP00000093830

UniGene: Mm.491229