Recombinant Mouse Natriuretic peptides A (Nppa)

中文名稱：

小鼠Nppa重組蛋白
貨號：

CSB-EP016020MO
說明書：

MSDS Datasheet
規格：

￥2328
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

Nppa
Uniprot No.：

P05125
種屬：

Mus musculus (Mouse)
蛋白長度：

Full Length of Mature Protein
來源：

E.coli
分子量：

18.4 kDa
表達區域：

123-150aa
氨基酸序列

SLRRSSCFGGRIDRIGAQSGLGCNSFRY
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-KSI-tagged
產品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Hormone that plays a key role in mediating cardio-renal homeostasis, and is involved in vascular remodeling and regulating energy metabolism. Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins, such as PRKG1, that drive various biological responses. Regulates vasodilation, natriuresis, diuresis and aldosterone synthesis and is therefore essential for regulating blood pressure, controlling the extracellular fluid volume and maintaining the fluid-electrolyte balance. Also involved in inhibiting cardiac remodeling and cardiac hypertrophy by inducing cardiomyocyte apoptosis and attenuating the growth of cardiomyocytes and fibroblasts. Plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus, and thus prevents pregnancy-induced hypertension. In adipose tissue, acts in various cGMP- and PKG-dependent pathways to regulate lipid metabolism and energy homeostasis. This includes upregulating lipid metabolism and mitochondrial oxygen utilization by activating the AMP-activated protein kinase (AMPK), and increasing energy expenditure by acting via MAPK11 to promote the UCP1-dependent thermogenesis of brown adipose tissue. Binds the clearance receptor NPR3 which removes the hormone from circulation.; May have a role in cardio-renal homeostasis through regulation of natriuresis, diuresis, vasodilation, and inhibiting aldosterone synthesis. In vitro, promotes the production of cGMP and induces vasodilation. May promote natriuresis, at least in part, by enhancing prostaglandin E2 synthesis resulting in the inhibition of renal Na+-K+-ATPase. However reports on the involvement of this peptide in mammal blood volume and blood pressure homeostasis are conflicting; according to a report, in vivo it is not sufficient to activate cGMP and does not inhibit collecting duct transport nor effect diuresis and natriuresis. Appears to bind to specific receptors that are distinct from the receptors bound by atrial natriuretic peptide and vessel dilator. Possibly enhances protein excretion in urine by decreasing proximal tubular protein reabsorption.; May have a role in cardio-renal homeostasis through regulation of natriuresis, diuresis, and vasodilation. In vitro, promotes the production of cGMP and induces vasodilation. May promote natriuresis, at least in part, by enhancing prostaglandin E2 synthesis resulting in the inhibition of renal Na+-K+-ATPase. However reports on the involvement of this peptide in mammal blood volume and blood pressure homeostasis are conflicting; according to a report it is not sufficient to activate cGMP and does not inhibit collecting duct transport nor effect diuresis and natriuresis. Appears to bind to specific receptors that are distinct from the receptors bound by the atrial natriuretic and long-acting natriuretic peptides. Possibly functions in protein excretion in urine by maintaining the integrity of the proximal tubules and enhancing protein excretion by decreasing proximal tubular protein reabsorption.; May have a role in cardio-renal homeostasis through regulation of diuresis and inhibiting aldosterone synthesis. In vitro, promotes the production of cGMP and induces vasodilation. May promote natriuresis, at least in part, by enhancing prostaglandin E2 synthesis resulting in the inhibition of renal Na+-K+-ATPase. May have a role in potassium excretion but not sodium excretion (natriuresis). Possibly enhances protein excretion in urine by decreasing proximal tubular protein reabsorption.; Hormone produced in the kidneys that appears to be important for maintaining cardio-renal homeostasis. Mediates vasodilation, natriuresis and diuresis primarily in the renal system, in order to maintain the extracellular fluid volume and control the fluid-electrolyte balance. Specifically binds and stimulates cGMP production by renal transmembrane receptors, likely NPR1. Urodilatin not ANP, may be the natriuretic peptide responsible for the regulation of sodium and water homeostasis in the kidney.; May have a role in cardio-renal homeostasis through regulation of natriuresis and vasodilation. In vivo promotes natriuresis and in vitro, vasodilates renal artery strips.; May have a role in cardio-renal homeostasis through regulation of natriuresis and vasodilation. In vivo promotes natriuresis and in vitro, vasodilates renal artery strips.; May have a role in cardio-renal homeostasis through regulation of regulation of natriuresis and vasodilation. In vivo promotes natriuresis. In vitro, vasodilates intestinal smooth muscle but not smooth muscle strips.; May have a role in cardio-renal homeostasis through regulation of natriuresis and vasodilation. In vivo promotes natriuresis. In vitro, selectively vasodilates intestinal and vascular smooth muscle strips.; May have a role in cardio-renal homeostasis through regulation of natriuresis and vasodilation. In vivo promotes natriuresis. In vitro, selectively vasodilates intestinal smooth muscle but not vascular smooth muscle strips.
基因功能參考文獻：
1. data demonstrate that gestational hypertension and lack of maternal ANP did not significantly impact the progression and regression of pregnancy-induced cardiac hypertrophy over gestation and postpartum in ANP(+/-) offspring. PMID: 29802597
2. The present study provides a novel insight into the potential underlying mechanisms of telmisartan-induced inhibition of cardiomyocyte hypertrophy, which involves inhibition of NFAT activation, nuclear translocation and the ANP/BNP cascade. PMID: 28447738
3. the developmental regulation and stress response of the Nppa-Nppb cluster involve the concerted action of multiple enhancers and epigenetic changes distributed across a structurally rigid regulatory domain. PMID: 27048739
4. we investigated pregnancies of ANP wild type, heterozygous, and knockout mice. Maternal blood pressure did not differ between genotypes, and fetal weight was unaffected. Placental weight was greater in ANP(-/-) versus ANP(+/+) mice. Therefore, in our hands, the ANP model does not express phenotypic features of preeclampsia with fetal growth restriction. PMID: 27238710
5. Data (including data from studies in knockout mice) suggest expression of ANP/BNP/GC-A (atrial natriuretic peptide, brain natriuretic peptide, and guanylyl cyclase-A) is important in embryonic neovascularization and organogenesis. PMID: 26517044
6. Goalpha plays a role in ANF sorting during intracellular vectorial transport and with the presence of a mechanism that preserves the molar relationship between cellular ANF and BNP. PMID: 25917834
7. ANP prevents cancer metastasis by inhibiting the adhesion of tumor cells to inflamed endothelial cells PMID: 25775533
8. Expression of NPPA splice variants is differentially regulated in the developing mouse heart. PMID: 25260157
9. NPPA acts on the NPR1 receptor, is expressed in mouse dorsal root and trigeminal ganglia, and the spinal cord. PMID: 25123163
10. Natriuretic peptides buffer renin-dependent hypertension. PMID: 24717731
11. Data indicate that the identified stress-responsive enhancer of Nppa and Nppb, the most common markers of heart failure, is a 650-bp fragment within 50 kb of the Nppa and Nppb loci. PMID: 24391132
12. Absence of maternal atrial natriuretic peptide leads to gestational hypertension and adaptive changes fetal cardiac growth and blood pressure regulation. PMID: 23981445
13. This study investigated a novel mechanism of vascular barrier protection by ANP via modulation of GEF-H1 function. PMID: 24352660
14. Data indicate that NRG-1beta up-regulated the gene expressions of Nkx2.5, GATA4, alpha-actin, MLC-2v, and ANF in a time-dependent regulation of ErbB/ERK1/2 pathways. PMID: 23606057
15. Atrial natriuretic peptide suppresses Th17 development through regulation of cGMP-dependent protein kinase and PI3K-AKT signaling pathways. PMID: 23327998
16. there is a gut-heart GLP-1R-dependent and ANP-dependent axis that regulates blood pressure and involves Epac2 PMID: 23542788
17. Characterize an estradiol-regulated interaction of NPPA with ERalpha in cardiomyocytes. PMID: 22962310
18. Data show that KO/TgV mice had significantly higher levels of proatrial natriuretic peptide in the heart compared with that in control KO/TgWT mice, indicating that the corin variant was defective in processing natriuretic peptides in vivo. PMID: 22987923
19. PDE5 inhibition did not alter atrial natriuretic peptide-stimulated cGMP in the resting heart but augmented it in the transaortic constriction heart. PMID: 22829024
20. Data suggest that Western diets (i.e., diets high in saturated fat and or salt) up-regulate expression of ANP in cardiomyocytes and increase its serum level. PMID: 21499941
21. Data suggest that a NPPA gradient originating in the oviduct induces sperm chemotaxis by binding to its receptor NPR1 and then activating PKG pathway, and plays a physiological role in fertilization. PMID: 21809340
22. Nkx2-5 and its responsive cis-regulatory DNA elements are essential for Atrial natriuretic factor expression selectively in the developing heart. PMID: 21930795
23. These results strongly suggest a protective role of ANP in the in vitro and in vivo models of ALI associated with gram-positive infection. PMID: 21051573
24. Atrial natriuretic peptide did not localize to uterine natural killer cells at gestational days 6 or 8, but did colocalize to uNK cells at gd10 and 12. PMID: 20959647
25. direct myocardial role for ANP/GC-A/cGMP to antagonize the Ca(2+) (i)-dependent hypertrophic growth response to Ang II, but not to beta-adrenergic stimulation PMID: 20352235
26. results suggest that particulate matter and O3 exposures, alone and combined, lead to different cardiac functional changes, and these unique changes are age-specific and dependent on Nppa and Npr1 genes PMID: 20540624
27. Upregulation of ANP mRNA in the kidney and heart of eNOS knockout mice PMID: 20403400
28. cGKIbeta signaling via IRAG is essential for regulation of smooth muscle tone and of intracellular calcium by NO and by atrial natriuretic peptide. PMID: 20080989
29. The ANP/NPR-A signaling pathway is upregulated in streptozotocin-induced diabetic mice, and contributes to the development of diabetic gastroparesis. PMID: 20039448
30. ANP-mediated increases in distal sodium delivery alone exert modest effects on sodium excretion PMID: 19906950
31. The upregulation of left ventricular atrial natriuretic peptide (ANP) serves as a molecular marker of cardiac hypertrophy. PMID: 11821706
32. Processing of pro-atrial natriuretic peptide by corin in cardiac myocytes. PMID: 11884416
33. Cooperative action of Tbx2 and Nkx2.5 inhibits ANF expression in the atrioventricular canal thus suppressing myocardial chamber formation PMID: 12023302
34. ANP acts as an anti-permeability factor by inhibiting the signaling functions of vascular permeability factor and by preserving the endothelial cell tight junctions functional morphology PMID: 12213803
35. HAND2 is an upstream transcriptional regulator of ANP expression PMID: 12392994
36. Data suggest that stretching and endothelin-1 are important stimuli to atrial natriuretic peptide (ANP) secretion from mouse atria, and the responsiveness of the ANP system to those stimuli are not coupled to the pathway involving heat shock protein 70. PMID: 12563028
37. Isoforms of PITX2 specifically regulate expression and repression of this factor, synergistically with Nkx2.5 PMID: 12692125
38. Effects of pressure overload on extracellular matrix expression in the heart of the atrial natriuretic peptide-null mouse. PMID: 12756220
39. Jumonji may play important roles in the down-regulation of ANF gene expression and in heart development PMID: 15542826
40. IL-18 induces cardiomyocyte hypertrophy via PI3K-dependent signaling, mediates ANF gene transcription, and has a role in inflammatory heart diseases including heart failure PMID: 15574430
41. data establish corin as the physiological pro-ANP convertase and indicate that corin deficiency may contribute to hypertensive heart disease (pro-ANP convertase corin) PMID: 15637153
42. local ANP/GC-A/cyclic GMP signaling counter-regulates MAPK/ERK- and calcineurin/nuclear factor of activated T cells-dependent pathways of cardiac myocyte growth in hypertensive eNOS-/- mice PMID: 15793309
43. activation of cardiac GC-A by locally secreted atrial and brain natriuretic peptides protects the heart from excessive cardiac remodeling by inhibiting the calcineurin-NFAT pathway. PMID: 15939815
44. high salt was able to increase plasma ANP levels and ventricular mRNA expression of ANP suggesting that ANP is an important determinant of the degree of salt-sensitivity observed in the proANP gene-disrupted animal PMID: 16132693
45. Loss of Hey1/2 leads to elevated GATA4/6 and ANF mRNA levels in embryoid bodies, while forced expression of Hey factors strongly represses expression of the GATA4 and GATA6 promoter. PMID: 16199874
46. The salt-sensitive hypertension in the ANP+/- mouse is exacerbatted,and possibly driven by the vasoconstrictive effects resulting from an upregulated endothelin-1/endothelin A receptor pathway. PMID: 16335784
47. ANP and AT1 receptor blockade attenuate angiotensin II-induced renomedullary interstitial cells proliferation and extracellular matrix synthesis more efficiently in the absence of neutral endopeptidase. PMID: 16582578
48. Results confirm the important role of ANP in the modulation of sympathetic nerve activity and its contribution to blood pressure homeostasis. PMID: 17482290
49. A significant increase in plasma oxytocin level was observed at 5 and 20 min after each central stimulation. PMID: 17524563
50. phosphorylation of Smad3 by protein kinase G is a potential molecular mechanism by which activation of ANP/cGMP/protein kinase G signaling disrupts TGF-beta1-induced nuclear translocation of pSmad3 and downstream events PMID: 17991884
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亞細胞定位：

[Long-acting natriuretic peptide]: Secreted.; [Vessel dilator]: Secreted.; [Kaliuretic peptide]: Secreted.; [Urodilatin]: Secreted.; [Atrial natriuretic peptide]: Secreted. Perikaryon. Cell projection.; [Atriopeptin-3]: Secreted.
蛋白家族：

Natriuretic peptide family
數據庫鏈接：

KEGG: mmu:230899

STRING: 10090.ENSMUSP00000099520

UniGene: Mm.482123