Recombinant Mouse N-lysine methyltransferase SMYD2 (Smyd2)

中文名稱：

小鼠Smyd2重組蛋白
貨號：

CSB-YP854521MO
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

小鼠Smyd2重組蛋白
貨號：

CSB-EP854521MO
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

小鼠Smyd2重組蛋白
貨號：

CSB-EP854521MO-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Smyd2重組蛋白
貨號：

CSB-BP854521MO
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Smyd2重組蛋白
貨號：

CSB-MP854521MO
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Smyd2
Uniprot No.：

Q8R5A0
別名：

Smyd2; N-lysine methyltransferase SMYD2; EC 2.1.1.-; Histone methyltransferase SMYD2; EC 2.1.1.354; SET and MYND domain-containing protein 2
種屬：

Mus musculus (Mouse)
蛋白長度：

full length protein
表達區域：

1-433
氨基酸序列

MRAEARGGLE RFCSAGKGRG LRALRPFHVG DLLFSCPAYA CVLTVGERGH HCECCFARKE GLSKCGRCKQ AFYCDVECQK EDWPLHKLEC SSMVVLGENW NPSETVRLTA RILAKQKIHP ERTPSEKLLA VREFESHLDK LDNEKKDLIQ SDIAALHQFY SKYLEFPDHS SLVVLFAQVN CNGFTIEDEE LSHLGSAIFP DVALMNHSCC PNVIVTYKGT LAEVRAVQEI HPGDEVFTSY IDLLYPTEDR NDRLRDSYFF TCECRECTTK DKDKAKVEVR KLSSPPQAEA IRDMVRYARN VIEEFRRAKH YKSPSELLEI CELSQEKMSS VFEDSNVYML HMMYQAMGVC LYMQDWEGAL KYGQKIIKPY SKHYPVYSLN VASMWLKLGR LYMGLENKAA GEKALKKAIA IMEVAHGKDH PYISEIKQEI ESH
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Protein-lysine N-methyltransferase that methylates both histones and non-histone proteins, including p53/TP53 and RB1. Specifically trimethylates histone H3 'Lys-4' (H3K4me3) in vivo. The activity requires interaction with HSP90alpha. Shows even higher methyltransferase activity on p53/TP53. Monomethylates 'Lys-370' of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates RB1 at 'Lys-860'.
基因功能參考文獻：
1. Our data indicate that Smyd2 has a critical role downstream of Myc in acute myeloid leukemia PMID: 27655694
2. two positive feedback loops that integrate epigenetic regulation and renal inflammation in cyst development: SMYD2/IL-6/STAT3/SMYD2 and SMYD2/TNF-alpha/NF-kappaB/SMYD2. PMID: 28604386
3. a pivotal role for SMYD2 in promoting pancreatic cancer. PMID: 26988419
4. Data show that histone methyltransferase Smyd2 inhibits macrophage interleukin 6 (IL-6) and tumor necrosis factor alpha (TNF-alpha) production. production. PMID: 25583990
5. Smyd2 is a lysine methyltransferase which has, next to its nuclear activity, specific regulatory functions in the cytoplasm of heart and skeletal muscle cells. PMID: 23047121
6. Sin3A-mediated deacetylation within the coding regions of active genes is directly linked to the histone methyltransferase activity of Smyd2 [Symd2] PMID: 16805913
7. results show that Smyd2 negatively regulates p53-responsive genes in a p53-dependent manner PMID: 17108971
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亞細胞定位：

Cytoplasm, cytosol. Nucleus.
蛋白家族：

Class V-like SAM-binding methyltransferase superfamily
組織特異性：

Highly expressed in heart, skeletal muscle and brain tissue. During cardiac development, it is differentially expressed with highest expression in the neonatal heart while very low expression is detected at E12.5 and adult. Specifically expressed in cardi
數據庫鏈接：

KEGG: mmu:226830

STRING: 10090.ENSMUSP00000027897

UniGene: Mm.156895