Recombinant Mouse Monoglyceride lipase (Mgll)

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中文名稱：

Recombinant Mouse Monoglyceride lipase (Mgll)
貨號：

CSB-EP013787MO
說明書：

MSDS Datasheet
規(guī)格：

￥2328
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

Greater than 95% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

Mgll
Uniprot No.：

O35678
別名：

MGL;Monoacylglycerol lipase (MAGL)
種屬：

Mus musculus (Mouse)
蛋白長度：

Full Length
來源：

E.coli
分子量：

37.5 kDa
表達(dá)區(qū)域：

1-303aa
氨基酸序列

MPEASSPRRTPQNVPYQDLPHLVNADGQYLFCRYWKPSGTPKALIFVSHGAGEHCGRYDELAHMLKGLDMLVFAHDHVGHGQSEGERMVVSDFQVFVRDVLQHVDTIQKDYPDVPIFLLGHSMGGAISILVAAERPTYFSGMVLISPLVLANPESASTLKVLAAKLLNFVLPNMTLGRIDSSVLSRNKSEVDLYNSDPLVCRAGLKVCFGIQLLNAVARVERAMPRLTLPFLLLQGSADRLCDSKGAYLLMESSRSQDKTLKMYEGAYHVLHRELPEVTNSVLHEVNSWVSHRIAAAGAGCPP
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

N-terminal 6xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評價

靶點(diǎn)詳情

功能：

Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain. Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth.
基因功能參考文獻(xiàn)：
1. Neuronal and astrocytic monoacylglycerol lipase contributes to 2-arachidonoylglycerol clearance and prevent CB1 receptor over-stimulation in the cerebellum. PMID: 27775008
2. Results provide evidence that MGL deficiency causes complex changes in cholesterol metabolism and in the regulation of gut transit. PMID: 28380440
3. N-arachidonoyl ethanolamine and 2-arachidonoyl glycerol hydrolyzing enzymes, FAAH and MAGL, and the CB1 receptor link the endocannabinoid system to broader lipid signaling networks in contrasting ways, potentially altering neurotransmission and behavior independently of cannabinoid receptor signaling. PMID: 27109320
4. Results suggest that neuronal and astrocytic MAGL collaborate to terminate endocannabinoid-mediated synaptic suppression and prompt synapse-specificity of endocannabinoid signaling in the cerebellum. PMID: 27182552
5. Activities of adipose triglyceride lipase (ATGL), hormone sensitive lipolitic enzyme (HSL) and monoacylglycerol lipase (MGL) were significantly higher (51 %, 38 %, 49 %) in the DE group than the HF group (p < 0.05). MGL, there were no differences between the CO group, HF group, and DC group, with the DE group (70 %) being significantly higher (p < 0.05). PMID: 27596982
6. MGL in astrocytes is an important regulator of 2-arachidonoylglyerol levels, arachidonic acid availability, and neuroinflammation. PMID: 26565024
7. Genetic and pharmacological ablation of Magl attenuated centrally-mediated fever response. PMID: 26287872
8. the results indicate that global MGL deletion leads to systemic changes that produce a leaner phenotype and an improved serum metabolic profile. PMID: 25842377
9. This study showed that Genetic deletion of monoacylglycerol lipase leads to impaired cannabinoid receptor CBR signaling and anxiety-like behavior. PMID: 26223500
10. Suggest that organophosphate agents induce plasma hypertriglyceridemia in mouse through single or dual inhibition of FAAH or/and MAGL, apparently leading to overstimulation of cannabinoid signal regulating energy metabolism. PMID: 24361246
11. Inactivation of Monoacylglycerol lipase robustly suppressed production and accumulation of beta-amyloid (Abeta) associated with reduced expression of beta-site amyloid precursor protein cleaving enzyme 1 (BACE1) in a mouse model of Alzheimer's disease. PMID: 23122958
12. Data indicate that nerve growth factor (NGF) controls monoacylglycerol lipase (MGL) degradation in vitro and in vivo. PMID: 23319656
13. The results therefore suggest a role for intestinal MGL in whole body energy balance via regulation of food intake as well as metabolic rate. PMID: 22937137
14. MGL regulates 2-arachidonoylglycerol signaling rather broadly within a certain range of neural tissue, although MGL expression is heterogeneous and limited to a subset of nerve terminals and astrocytes. PMID: 22783023
15. results indicate that genetic deletion of MAGL causes profound changes in eCB signaling, long-term synaptic plasticity, and learning behavior. PMID: 21940435
16. pathway exists in brain where MAGL hydrolyzes 2-arachidonoylglycerol to generate arachidonate precursor pool for neuroinflammatory prostaglandins; found MAGL as metabolic node coupling endocannabinoid to prostaglandin signaling networks in nervous system PMID: 22021672
17. MGL deficiency impairs lipolysis and attenuates diet-induced insulin resistance PMID: 21454566
18. MAGL is the major regulator of 2- arachidonoylglycerol (2-AG) levels and signaling. PMID: 20855465
19. spatially confined MGL activity generates a 2-arachidonoyl glycerol sensing microdomain and configures 2-arachidonoyl glycerol signaling to promote axonal growth PMID: 20962221
20. sustained inactivation of monoacylglycerol lipase impairs specific endocannabinoid-mediated forms of synaptic plasticity; ligand diversification may be important for shaping the distinct functions and properties of endocannabinoid signaling pathways PMID: 20729846
21. Astrocytes express monoacylglycerol lipase, the main hydrolyzing enzyme of 2-arachidonoylglycerol. Pharmacological inhibition of MGL potentiates ATP-induced 2-AG production. PMID: 15371507
22. analysis of developmental and nutritional regulation of monoacylglycerol lipase and monoacylglycerol acyltransferase PMID: 17848545
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亞細(xì)胞定位：

Cytoplasm, cytosol. Membrane; Peripheral membrane protein.
蛋白家族：

AB hydrolase superfamily, Monoacylglycerol lipase family
組織特異性：

Ubiquitous.
數(shù)據(jù)庫鏈接：

KEGG: mmu:23945

STRING: 10090.ENSMUSP00000127374

UniGene: Mm.272197