Recombinant Mouse Kinetochore protein NDC80 homolog (Ndc80)

中文名稱：

小鼠Ndc80重組蛋白
貨號：

CSB-YP875172MO
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

小鼠Ndc80重組蛋白
貨號：

CSB-EP875172MO
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

小鼠Ndc80重組蛋白
貨號：

CSB-EP875172MO-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Ndc80重組蛋白
貨號：

CSB-BP875172MO
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Ndc80重組蛋白
貨號：

CSB-MP875172MO
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Ndc80
Uniprot No.：

Q9D0F1
別名：

Ndc80; Hec1; Kntc2; Kinetochore protein NDC80 homolog; Kinetochore protein Hec1; Kinetochore-associated protein 2
種屬：

Mus musculus (Mouse)
蛋白長度：

full length protein
表達區域：

1-642
氨基酸序列

MKRSSVSTCG AGRLSMQELR TLDLNKPGLY TPQTKERSTF GKLSTHKPTS ERKVSIFGKR TSGHGSRNSQ LGIFSSSEKI KDPRPLNDKA FIQQCIRQLY EFLTENGYVY SVSMKSLQAP STKEFLKIFA FLYGFLCPSY ELPGTKCEEE VPRIFKALGY PFTLSKSSMY TVGAPHTWPH IVAALVWLID CIKIDTAMKE SSPLFDDGQL WGEETEDGIK HNKLFLEYTK KCYEKFMTGA DSFEEEDAEL QAKLKDLYKV DASKLESLEA ENKALNEQIA RLEEEREREP NRLMSLKKLK ASLQADVQNY KAYMSNLESH LAVLKQKSNS LDEEIGRVEQ ECETVKQENT RLQSIVDNQK YSVADIERIN HEKNELQQTI NKLTKDLEAE QQQMWNEELK YARGKEAIEA QLAEYHKLAR KLKLIPKGAE NSKGYDFEIK FNPEAGANCL VKYRTQVYAP LKELLNESEE EINKALNKKR HLEDTLEQLN TMKTESKNTV RMLKEEIQKL DDLHQQAVKE AEEKDKKSAS ELESLEKHKH LLESGVNDGL SEAMDELDAV QREYQLTVKT TTEERRKVEN NLQRLLEMVA THVGSLEKHL EEENAKADRE YEEFMSEDLL ENIREMAEKY KRNAAQLKAP DK
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules. Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules.
基因功能參考文獻：
1. These data strongly indicated that KNTC2 is a promising target for the treatment of HCC and that phosphorylated HH3 at serine 10 is one of the target engagement markers for KNTC2. PMID: 28843857
2. Further investigation of the phosphoregulation of meiotic Kif4 revealed that Aurora Kinase and Cdk activity is critical for Kif4 kinetochore localization and interaction with Ndc80 and CENP-C. Finally, Kif4 protein but not gene expression was found to be upregulated with age, suggesting a role for this protein in the decline of oocyte quality with age PMID: 28125646
3. Hec1-dependent cyclin B2 stabilization during early metaphase is important for early-stage spindle assembly. PMID: 23541922
4. Disruption of Ndc80 function by either siRNA injection or antibody injection resulted in severe chromosome misalignment, spindle disruption, and precocious polar body extrusion. PMID: 21600073
5. overexpression of Hec1 results in mitotic checkpoint hyperactivation that leads to aneuploidy sufficient to generate tumors PMID: 18940925
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亞細胞定位：

Nucleus. Chromosome, centromere, kinetochore.
蛋白家族：

NDC80/HEC1 family
組織特異性：

Expressed in spleen, testis and thymus.
數據庫鏈接：

KEGG: mmu:67052

STRING: 10090.ENSMUSP00000024851

UniGene: Mm.225956