Recombinant Mouse Interferon-inducible double stranded RNA-dependent protein kinase activator A (Prkra)

中文名稱：

小鼠Prkra重組蛋白
貨號(hào)：

CSB-YP896234MO
說(shuō)明書：

MSDS Datasheet
規(guī)格：
來(lái)源：

Yeast
其他：

中文名稱：

小鼠Prkra重組蛋白
貨號(hào)：

CSB-EP896234MO
說(shuō)明書：

MSDS Datasheet
規(guī)格：
來(lái)源：

E.coli
其他：

中文名稱：

小鼠Prkra重組蛋白
貨號(hào)：

CSB-EP896234MO-B
說(shuō)明書：

MSDS Datasheet
規(guī)格：
來(lái)源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Prkra重組蛋白
貨號(hào)：

CSB-BP896234MO
說(shuō)明書：

MSDS Datasheet
規(guī)格：
來(lái)源：

Baculovirus
其他：

中文名稱：

小鼠Prkra重組蛋白
貨號(hào)：

CSB-MP896234MO
說(shuō)明書：

MSDS Datasheet
規(guī)格：
來(lái)源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

Prkra
Uniprot No.：

Q9WTX2
別名：

Prkra; Rax; Interferon-inducible double-stranded RNA-dependent protein kinase activator A; PKR-associated protein X; PKR-associating protein X; RAX; Protein activator of the interferon-induced protein kinase; Protein kinase; interferon-inducible double-stranded RNA-dependent activator
種屬：

Mus musculus (Mouse)
蛋白長(zhǎng)度：

full length protein
表達(dá)區(qū)域：

1-313
氨基酸序列

MSHSRHRAEA PPLQREDSGT FSLGKMITAK PGKTPIQVLH EYGMKTKNIP VYECERSDVQ VHVPTFTFRV TVGDITCTGE GTSKKLAKHR AAEAAINILK ANASICFAVP DPLMPDPSKQ PKNQLNPIGS LQELAIHHGW RLPEYTLSQE GGPAHKREYT TICRLESFME TGKGASKKQA KRNAAEKFLA KFSNISPENH ISLTNVVGHS LGCTWHSLRN SPGEKINLLK RSLLSLPNTD YIQLLSEIAS EQGFNITYLD IEELSANGQY QCLAELSTSP ITVCHGSGIS CGNAQSDAAH NALQYLKIIA ERK
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1. Activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Promotes UBC9-p53/TP53 association and sumoylation and phosphorylation of p53/TP53 at 'Lys-386' at 'Ser-392' respectively and enhances its activity in a EIF2AK2/PKR-dependent manner.
基因功能參考文獻(xiàn)：
1. PKR knock-out alleviates abnormal brain signaling and body weight gain in experimental obesity. PMID: 29795582
2. PACT interacts with LGP2 and this interaction is enhanced by encephalomyocarditis virus (EMCV) infection. In vitro interaction analyses using purified recombinant proteins confirmed that the single-stranded Theiler's murine encephalitis virus genome enhanced the interaction between LGP2 and PACT. PMID: 29032202
3. Data (including data from studies in transgenic/knockout mice) suggest that PACT/RAX (protein activator of protein kinase R) functions as negative regulator of PKR/Eif2ak2 (protein kinase R) in development of postnatal anterior pituitary tissue. PMID: 26414443
4. Deficiency of PKR diminished peripheral inflammatory responses following E. coli PMID: 26585788
5. These findings demonstrated a novel function of PACT/RAX in the regulation of neuronal migration. PMID: 25609658
6. The protein kinase PKR is critical for LPS-induced iNOS production but is dispensable for inflammasome activation in macrophages. PMID: 23401008
7. These observations identified a specific mutation that reduces steady-state levels of RAX protein and disrupts the dsRNA binding function of the protein, demonstrating the importance of the Prkra gene in various aspects of mouse development. PMID: 22194846
8. RAX phosphorylation at serine 18 is required for PKR activation and consequent translation inhibition PMID: 15299031
9. Study demonstrated an essential role of PACT in mammalian ear development and produced the first animal model. PMID: 16571658
10. The role of PACT in mediating gene induction, PKR activation, and apoptosis in response to diverse stimuli PMID: 18729737
11. The authors demonstrate for the first time that PACT is phosphorylated in response to tunicamycin and is responsible for PKR activation by direct interaction. PMID: 19007793
12. Study revealed a physiologically significant role for PACT in cell proliferation and an essential role of a dsRNA-binding protein in mammalian pituitary expansion. PMID: 19541653
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亞細(xì)胞定位：

Cytoplasm, perinuclear region. Cytoplasm.
蛋白家族：

PRKRA family
組織特異性：

Expressed in brain, heart, kidney, liver, lung, muscle, spleen and testis.
數(shù)據(jù)庫(kù)鏈接：

KEGG: mmu:23992

STRING: 10090.ENSMUSP00000002808

UniGene: Mm.277250