Recombinant Mouse Inactive serine/threonine-protein kinase VRK3 (Vrk3)

中文名稱：

小鼠Vrk3重組蛋白
貨號：

CSB-YP809103MO
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

小鼠Vrk3重組蛋白
貨號：

CSB-EP809103MO
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

小鼠Vrk3重組蛋白
貨號：

CSB-EP809103MO-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Vrk3重組蛋白
貨號：

CSB-BP809103MO
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Vrk3重組蛋白
貨號：

CSB-MP809103MO
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Vrk3
Uniprot No.：

Q8K3G5
別名：

Vrk3Inactive serine/threonine-protein kinase VRK3; Serine/threonine-protein pseudokinase VRK3; Vaccinia-related kinase 3
種屬：

Mus musculus (Mouse)
蛋白長度：

full length protein
表達區域：

1-453
氨基酸序列

MISFCPVCGK SVKVSFKFCP YCGKALPVEE DGGTQSAVTP HVSSVPGSRR DLNSSFETSP KKVKCSHTVT SLPLSRHSDC DSSGSDNTLT SPDRATGTRS RPLTPKGSPL SNRQSPQTLK RTRVTTSLQA LATGTELTDQ NGKHWTLGAL QIRDDQGILY EAEPTSAVPS ESRTQKWRFS LKLDSKDGRL FNEQNFFQRV AKPLQVNKWK KQFLLPLLAI PTCIGFGIHQ DKYRFLVFPS LGRSLQSALD DNPKHVVSER CVLQVACRLL DALEYLHENE YVHGNLTAEN VFVNPEDLSQ VTLVGYGFTY RYCPGGKHVA YKEGSRSPHD GDLEFISMDL HKGCGPSRRS DLQTLGYCML KWLYGSLPWT NCLPNTEKIT RQKQKYLDSP ERLVGLCGRW NKASETLREY LKVVMALNYE EKPPYATLRN SLEALLQDMR VSPYDPLDLQ MVP
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Inactive kinase that suppresses ERK activity by promoting phosphatase activity of DUSP3 which specifically dephosphorylates and inactivates ERK in the nucleus.
基因功能參考文獻：
1. Stress-induced nuclear translocation of CDK5 suppresses neuronal death by downregulating ERK activation via VRK3 phosphorylation PMID: 27346674
2. Finally, downregulation of hvrk3 using siRNA reduced the amplitude and lengthened the period of the cellular bioluminescence rhythm. Taken together, these data suggest that VRK3 plays a role in setting the amplitude and period length of circadian rhythms in mammalian cells. PMID: 28412365
3. These data suggest that VRK3-mediated phosphorylation of BAF may facilitate DNA replication or gene expression by facilitating the dissociation of nuclear envelope proteins and chromatin during interphase. PMID: 25899223
4. various other rodent adult tissues and embryos possess a common signaling mechanism which is involved in an indirect regulation of ERK activity by VRK3-mediated vaccinia H1-related (VHR) phosphatase activity PMID: 18035061
亞細胞定位：

Nucleus.
蛋白家族：

Protein kinase superfamily, CK1 Ser/Thr protein kinase family, VRK subfamily
組織特異性：

Expressed in liver, kidney, muscle, thymus, and bone marrow. Weakly expressed in spleen.
數據庫鏈接：

KEGG: mmu:101568

STRING: 10090.ENSMUSP00000002275

UniGene: Mm.28816