E. coli biotin ligase
(BirA) is highly specific in covalently attaching biotin to the 15
amino
acid AviTag peptide. This recombinant protein was biotinylated in
vivo
by AviTag-BirA technology, which method is BriA catalyzes amide
linkage
between the biotin and the specific lysine of the AviTag.
The tag type will
be
determined during production process. If you have specified tag
type, please tell us and we will develop the specified tag
preferentially.
產品提供形式:
Lyophilized
powder
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Note: We will
preferentially ship the format that we have in stock, however,
if you have any special requirement for the format, please
remark your requirement when placing the order, we will prepare
according to your demand.
復溶:
We recommend that this vial be briefly centrifuged
prior
to opening to bring the contents to the bottom. Please reconstitute
protein in deionized sterile water to a concentration of 0.1-1.0
mg/mL.We recommend to add 5-50% of glycerol (final concentration)
and
aliquot for long-term storage at -20℃/-80℃. Our default final
concentration of glycerol is 50%. Customers could use it as
reference.
儲存條件:
Store at -20°C/-80°C upon receipt, aliquoting is
necessary for
mutiple use. Avoid repeated freeze-thaw cycles.
保質期:
The shelf life is related to many factors, storage
state,
buffer ingredients, storage temperature and the stability of the
protein
itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C.
The
shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期:
Delivery time may
differ from different purchasing way or location, please kindly
consult your local distributors for specific delivery time.
Note: All of our
proteins are default shipped with normal blue ice packs, if you
request to ship with dry ice, please communicate with us in
advance
and extra fees will be charged.
注意事項:
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet :
Please contact us to get it.
產品評價
靶點詳情
基因功能參考文獻:
C57Bl/6-engrailed-1 (En1+/-) mice do not display the neurodegenerative phenotype, suggesting that susceptibility to En1 heterozygosity is genetically regulated. PMID: 27550741
En1 is important for ventral nuclei of the lateral lemniscus neuron development and survival. PMID: 26914477
in absence of En1 and Pitx3, only a limited number of Mesodiencephalic dopaminergic neurons are present in mouse embryo. PMID: 28800615
Engrailed plays an evolutionarily conserved role in the separate innervation of vertebrate epaxial-hypaxial muscle. PMID: 28807781
FGFR Inhibitor Ameliorates Hypophosphatemia and Impaired Engrailed-1/Wnt Signaling in FGF2 High Molecular Weight Isoform PMID: 26762209
En1-null cells also fail to express the transcription factor FoxP1, suggesting that FoxP1 lies downstream of En1. PMID: 26542008
The homeoprotein Engrailed is not only a survival factor for mesencephalic dopaminergic (mDA) neurons during development, but continues to exert neuroprotective and physiological functions in adult mDA neurons. [Review] PMID: 26459030
En1+/Otx2 Mutants Exhibit Increased Intra-Individual Fluctuation in Activity, Habituation, Risk-Taking Behavior, Sociability, and Hedonic-Like Behavior. PMID: 25241801
En1 is involved in two distinct molecular mechanisms that regulate the establishment of neuronal connectivity to limb-musculature. PMID: 25710467
low-frequency non-coding variant near a novel locus, EN1, with an effect on bone mineral density--which was also associated with a decreased risk of fracture PMID: 26367794
The findings of this study support a progressive retrograde degeneration of En1(+/-) nigrostriatal neurons, akin to what is suggested to occur in Parkinson disease. PMID: 25281317
This study identified En1 as a gene essential for MNTB neuron development and Sound localization. PMID: 24048834
Two crucial mediators of mesodiencephalic dopaminergic neuronal development, En1 and Pitx3, interact in dopaminergic subset specification. PMID: 23863478
Analysis of an allelic series of conditional mutants showed that En1 is the predominant functional En paralog in maturing 5-HT neurons PMID: 22674259
En1 and En2 act together in multiple cerebellar cell types to determine overall growth and formation of particular fissures. PMID: 22564796
En1 has a role in regulation of cranial morphogenesis and cell fate at the neural crest-mesoderm boundary PMID: 22395741
Engrailed homeoprotein recruits the adenosine A1 receptor to potentiate ephrin A5 function in retinal growth cones. PMID: 22147955
This study characterized En1 and En2 expression around birth and at postnatal day (P) 21 during the period when the cerebellum undergoes a remarkable transformation from a smooth ovoid structure to a highly foliated structure. PMID: 21431469
The data of this study suggested that in En1+/- mice nigrostriatal degeneration in the substantia nigra is functionally compensated. PMID: 21104462
In summary, This study suggested that En1/2 are master regulators of three-dimensional organization of the cerebellum and coordinately regulate morphology, patterned gene expression, and afferent topography. PMID: 20668186
Dynamic expression and heterogeneous intracellular location of En-1 during late mouse embryonic development PMID: 19940436
genes are fundamental to patterning cerebellum foliation throughout the mediolateral axis and act late in development PMID: 20081196
Engrailed-1 null mutations lead to perinatal lethality accompanied by near-total absence of cerebellar and caudal midbrain structures, but these cerebellar phenotypes are nearly completely suppressed on a C57BL/6J genetic background PMID: 12832534
Pax6 and En1 have essential roles in establishing the recurrent inhibitory circuit between motor neurons and Renshaw cells PMID: 14762144
Engrailed genes are cell-autonomously required to prevent apoptosis in mesencephalic dopaminergic neurons PMID: 15175251
EN1 is a novel modulator of calvarial osteoblast differentiation and proliferation. PMID: 16319118
Viable and fertile mice, heterozygous null for Engrailed-1 and homozygous null for Engrailed-2 (En1+/-;En2-/-), have an adult phenotype that resembles key pathological features of Parkinson's disease. PMID: 17015829
Background effects in the En1 mouse knock-out provides insight as to possible molecular mechanisms and gender differences associated with autism. PMID: 17055592
Loss and gain of function experiments firmly establish that En1/2 is a true survival factor for dopaminergic neurons in vivo. PMID: 17267560
Genetic subdivision of the tectum and cerebellum into functionally related regions is based on differential sensitivity to En1. PMID: 17537797
expression of shRNA against GPM6A markedly reduced the expression of neuroectodermal-associated genes (OTX1, Lmx1b, En1, Pax2, Pax5, Sox1, Sox2, and Wnt1), and also the number of neural stem cells (NSC) derived from D3mshM6A cells PMID: 18522499
The results of this study indicating Engrailed genes as survival factors for mature dopaminergic neurons. PMID: 18585948
Progressive loss of dopaminergic neurons in the ventral midbrain of adult mice heterozygote for Engrailed1. PMID: 18585951
Studies reveal that En1/2 are fundamental components of the genetic pathways that pattern the two intersecting coordinate systems of the cerebellum, morphological divisions and the molecular code. PMID: 19020009
Data show that cell death in Engrailed-deficient mesDA neurons in heterozygote animals (En1+/-;En2-/-)is a result of higher P75NTR expression and the loss of Erk1/2 activity. PMID: 19291307