Recombinant Mouse Glycine receptor subunit alpha-1 (Glra1), partial

中文名稱：

小鼠Glra1重組蛋白
貨號：

CSB-YP717517MO
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

小鼠Glra1重組蛋白
貨號：

CSB-EP717517MO
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

小鼠Glra1重組蛋白
貨號：

CSB-EP717517MO-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Glra1重組蛋白
貨號：

CSB-BP717517MO
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Glra1重組蛋白
貨號：

CSB-MP717517MO
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Glra1
Uniprot No.：

Q64018
別名：

Glra1Glycine receptor subunit alpha-1; Glycine receptor 48 kDa subunit; Glycine receptor strychnine-binding subunit
種屬：

Mus musculus (Mouse)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Glycine receptors are ligand-gated chloride channels. Channel opening is triggered by extracellular glycine. Channel opening is also triggered by taurine and beta-alanine. Channel characteristics depend on the subunit composition; heteropentameric channels are activated by lower glycine levels and display faster desensitization. Plays an important role in the down-regulation of neuronal excitability. Contributes to the generation of inhibitory postsynaptic currents. Channel activity is potentiated by ethanol. Potentiation of channel activity by intoxicating levels of ethanol contribute to the sedative effects of ethanol.
基因功能參考文獻：
1. These findings suggest that spinal alpha1 GlyR is a potential target for cannabinoid analgesia in chronic inflammatory pain. PMID: 29407767
2. The results of this study suggested that the Glra1 activation can regulate receptor diffusion and cluster size at inhibitory synapses in mature stage, providing not only new insights into the postsynaptic mechanism of shifting inhibitory neurotransmission but also the inhibitory synaptic plasticity in mature nervous system PMID: 28197549
3. Glra1 receptor Beta8-Beta9 loop is an essential regulator of conformational rearrangements in ion channel opening and closing. PMID: 28724750
4. Absence of GlyRalpha1 input unmasked a serial and a direct feedforward GABAAergic modulation in PV5 RGCs, reflecting a complex interaction between glycinergic and GABAAergic inhibition. PMID: 25231618
5. Results suggest an important function of alpha1 GlyR on sedative effects of ethanol and confirms the role of KK385-386 residues in these effects PMID: 24801766
6. Meanwhile, neonatal incision significantly decreased the density of tonic GlyR-mediated current only in the presumed glutamatergic population during adulthood. PMID: 23639821
7. Histamine is an inverse agonist at strychnine sensitive glycine receptors. PMID: 23603522
8. Mutation of a zinc-binding residue in the glycine receptor alpha1 changes ethanol sensitivity in vitro and alcohol consumption in Glra1(D80A) knock-in mice. PMID: 23230213
9. TM3-4 loop subdomains are important for functional reconstitution of glycine receptors by independent domains PMID: 22995908
10. The distinct developmental regulation of GlyRalpha1 in the murine cochlea advocates a contribution of these chloride channels to efferent olivocochlear innervation. PMID: 21850450
11. Mutations in the GlyR alpha-1 subunit, M287L and Q266I, resulted in a small but general impairment of glycine action, that is most evident in the glycine-induced maximal currents. PMID: 22037201
12. Mutations in the GlyR alpha-1 subunit, M287L and Q266I, decreased specific behavioral actions of ethanol and altered other nonethanol behaviors, demonstrating the importance of GlyR function in diverse neuronal systems. PMID: 22037202
13. these data suggest most glycine receptors and glycinergic synapses in the superficial dorsal horn contain alpha1 subunits and few are composed exclusively of alpha3 subunits. PMID: 21486794
14. The values of breathing frequency, tidal volume, and minute ventilation in newborn Glra1-deficient oscillator mice decrease rapidly during postnatal development. PMID: 12380014
15. The new Glra1 mutation appears to affect glycine's inhibitory neurotransmission in the central nervous system (CNS) of the nmf11 homozygotes, which suffer from a severe startle disease-related phenotype and die by postnatal day 21. PMID: 16964444
16. Thus, the hyperekplexia phenotype of Glra1(D80A) mice is due to the loss of Zn(2+) potentiation of alpha1 subunit containing GlyRs, indicating that synaptic Zn(2+) is essential for proper in vivo functioning of glycinergic neurotransmission. PMID: 17114051
17. These results show that insulin has a novel regulatory action on the potency of glycine for ionotropic glycine receptors. PMID: 17308032
18. Our results suggest crosstalk between postsynaptic and presynaptic elements, leading to the developmental regulation of the presynaptic terminal neurotransmitter content according to the level of postsynaptic GlyR aggregation. PMID: 18445051
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相關疾?。?/div>
Defects in Glra1 are the cause of the spasmodic (spd) phenotype, a mouse mutant which resembles the human neurological disease, hyperekplexia (or startle disease (STHE)) (PubMed:7920629). Defects in Glra1 are the cause of the lethal oscillator (spd-ot) phenotype. Mutant mice display a fine motor tremor and muscle spasms that begin at 2 weeks of age and progressively worsen, resulting in death by 3 weeks of age (PubMed:7874121). Heterozygous mice show an increased acoustic startle response (PubMed:9145798). Neurons from homozygous oscillator mice have dramatically reduced amplitude and frequency of glycinergic inhibitory postsynaptic currents (PubMed:16672662). The oscillator phenotype is due to the complete absence of Glra1 protein (PubMed:9145798).
亞細胞定位：

Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell junction, synapse. Perikaryon. Cell projection, dendrite. Cell membrane; Multi-pass membrane protein.
蛋白家族：

Ligand-gated ion channel (TC 1.A.9) family, Glycine receptor (TC 1.A.9.3) subfamily, GLRA1 sub-subfamily
組織特異性：

Detected in spinal cord neurons. Detected in brain stem neurons. Detected at lower levels in hippocampus and cerebellum. Detected in the inner plexiform layer of the retina (at protein level).
數據庫鏈接：

KEGG: mmu:14654

STRING: 10090.ENSMUSP00000099777

UniGene: Mm.89320