E. coli biotin ligase
(BirA) is highly specific in covalently attaching biotin to the 15
amino
acid AviTag peptide. This recombinant protein was biotinylated in
vivo
by AviTag-BirA technology, which method is BriA catalyzes amide
linkage
between the biotin and the specific lysine of the AviTag.
The tag type will
be
determined during production process. If you have specified tag
type, please tell us and we will develop the specified tag
preferentially.
產(chǎn)品提供形式:
Lyophilized
powder
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Note: We will
preferentially ship the format that we have in stock, however,
if you have any special requirement for the format, please
remark your requirement when placing the order, we will prepare
according to your demand.
復(fù)溶:
We recommend that this vial be briefly centrifuged
prior
to opening to bring the contents to the bottom. Please reconstitute
protein in deionized sterile water to a concentration of 0.1-1.0
mg/mL.We recommend to add 5-50% of glycerol (final concentration)
and
aliquot for long-term storage at -20℃/-80℃. Our default final
concentration of glycerol is 50%. Customers could use it as
reference.
儲存條件:
Store at -20°C/-80°C upon receipt, aliquoting is
necessary for
mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期:
The shelf life is related to many factors, storage
state,
buffer ingredients, storage temperature and the stability of the
protein
itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C.
The
shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期:
Delivery time may
differ from different purchasing way or location, please kindly
consult your local distributors for specific delivery time.
Note: All of our
proteins are default shipped with normal blue ice packs, if you
request to ship with dry ice, please communicate with us in
advance
and extra fees will be charged.
注意事項:
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet :
Please contact us to get it.
產(chǎn)品評價
靶點詳情
功能:
Essential for the control of the cell cycle at the G2/M (mitosis) transition.
基因功能參考文獻:
The results show that Cyclin B1 is essential for mitosis in mammalian cells and that its spatial localization has a crucial role in triggering mitosis at the correct time, even when Cdk1 can be regulated by the antagonistic Wee1-Myt1-Cdc25 pathways. PMID: 29074707
Results indicate that CCNB1 is critically required for the proliferation of gonocytes and spermatogonia but may be redundant in meiosis of spermatocytes in mouse spermatogenesis. PMID: 29072697
Results provide evidence that cdc2/cyclin B1 kinase activation was synchronous with the initial appearance of cytoskeletal lesions in mouse with Niemann-Pick disease type C. PMID: 29058287
Inhibition of CDK7 bypasses spindle assembly checkpoint via premature cyclin B degradation during oocyte meiosis. PMID: 27693251
timing of Ccnb1 mRNA translation in mouse oocytes is dependent on the presence of transcripts with different 3' untranslated regions PMID: 28808066
AURKA induced phosphorylation and recruitment of CDC25B to MTOCs prior to p-Cyclin B1-Ser123, and this sequential regulation is essential for the commitment of the oocytes to resume meiosis. PMID: 25349079
Parvovirus-induced depletion of cyclin B1 prevents mitotic entry of infected cells. PMID: 24415942
Cyclin B1/Cdk1-mediated phosphorylation of mitochondrial substrates allows cells to sense and respond to increased energy demand for G2/M transition and, subsequently, to upregulate mitochondrial respiration for successful cell-cycle progression. PMID: 24746669
sticky siRNAs against survivin and cyclin B1 efficiently blocks growth of established subcutaneaous B16-F10 tumors as well as formation and dissemination of melanoma lung metastases. PMID: 23835136
Inhibitions of Aurora B and Cyclin-dependent kinase 1 activity in vertebrate cells also have opposite effects on the timing of abscission. PMID: 23948252
It was concluded that insensitivity of mouse germinal vesicle oocytes to cyclohexidine was due to the presence of sufficient cyclin B1, and that cyclin B1 level in such oocytes was maintained by an equilibrium between synthesis and degradation. PMID: 22444557
Data show nuclear accumulation of cyclin B1 with a subsequent premature increase in G2/M kinase activity in Nipa-/- spermatocytes. PMID: 22696294
Data show that Cyclin B1 is synchronously degraded in whole oocytes and oocyte halves with a single bivalent PMID: 22125605
Data show that protein expression of cyclins D1 and B1 and cyclin-dependent kinases 2 and 4 was downregulated upon LXR activation. PMID: 21398609
Sustained spindle-assembly checkpoint response requires de novo transcription and translation of cyclin B1. PMID: 20927403
Results demonstrate that the transcriptional activity of HOX11 is regulated by phosphorylation of Thr247 in a cell cycle-specific manner and that this phosphorylation modulates the expression of the target gene, cyclin B1. PMID: 20846384
Linker histone binding to NASP controls the ability of HSPA2 to activate CDC2 for CDC2/cyclin B1 complex formation. PMID: 19553603
Nuclear accumulation is necessary for cyclin B1-dependent apoptosis. This is consistent with the idea that localization of cyclin B1 is among the factors determining the cellular decision to undergo apoptosis in response to DNA damage. PMID: 12424202
findings show that in the regenerating liver the circadian clock controls the expression of cell cycle-related genes that in turn modulate the expression of active Cyclin B1-Cdc2 kinase, a key regulator of mitosis PMID: 12934012
homologue disjunction in mouse oocytes is dependent on proteolysis of the separase inhibitor securin and cyclin B1 PMID: 14593421
demonstrated an expression pattern in preimplantation embryos opposite to a somatic G2/M cyclin, with peak levels in G1 PMID: 15214576
the ubiquitin-proteasome pathway plays important roles in oocyte meiosis resumption, spindle assembly, polar body emission, and pronuclear formation, probably by regulating cyclin B1 degradation and MAPK/p90rsk phosphorylation PMID: 15327787
Cyclin B1 suppression is a critical step in the mechanism by which farnesyl transferase inhibitors trigger apoptosis and robust antitumor efficacy PMID: 15548709
cyclin B1, phosphorylated cyclin B1 and p34cdc2 have similar distributions at some stages but different localizations at other stages during oocyte meiotic maturation and fertilization PMID: 15984162
These results indicate that Kruppel-like factor 5 accelerates mitotic entry in H-Ras-transformed cells by transcriptionally activating cyclin B1 and Cdc2, which leads to an increase in cyclin B1/Cdc2 kinase activity. PMID: 16102754
These experiments show that cyclinB1 is able to maintain a bipolar spindle even after sister chromatids had become separated and suggest an important role of hKid in this process. PMID: 16724106
The present study was aimed to investigate the possibility that selenium (Se)-induced oxidative stress mediated alterations in Cdc25c and p21 may cause modulations in the CDC2/Cyclin B1 complex responsible for G2/M phase checkpoint in spermatogenesis. PMID: 17160696
DEDD acts as a novel inhibitor of the mitotic Cdk1/cyclin B1 complex and is an impeder of cell mitosis, and its absence critically influences cell and body size via modulation of rRNA synthesis PMID: 17283331
Selenium-induced oxidative-stress-mediated modulations of thia cell cycle regulators may cause DNA damage. PMID: 17320365
cyclin B1 may have a role in esophageal squamous cell carcinoma invasive growth and metastasis PMID: 18048386
Data show that in mouse oocytes, excess securin causes stabilization of cyclin B and precocious entry into M-phase. PMID: 18364698
the suppression of nuclear import of cyclin B1, the induction of Wee1 kinase activity, and the transient nuclear accumulation of p21(CIP1/WAF1) may play important roles in the transient cell cycle delay in response to ionizing radiation PMID: 18425394
REVIEW. Some cyclins exhibit unique patterns of expression in germ cells that suggest possible concomitant distinct functions; embryonic lethality has obviated understanding of the germline functions of cyclin A2 and cyclin B1 PMID: 19001847
cyclin B1 has a role in inducing anticarcinogenic T-cell and antibody responses PMID: 19666607
cyclin B1 vaccination delays spontaneous cyclin B1(+) tumor growth and increases median survival of tumor-bearing p53 deficient mice. PMID: 19769738
PKA regulates cell cycle progression of fertilized eggs by interfering with the degradation of cyclin B. PMID: 15580572
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亞細胞定位:
Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.