Recombinant Mouse Fermitin family homolog 2 (Fermt2)

中文名稱：

小鼠Fermt2重組蛋白
貨號：

CSB-YP809298MO
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

小鼠Fermt2重組蛋白
貨號：

CSB-EP809298MO
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

小鼠Fermt2重組蛋白
貨號：

CSB-EP809298MO-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Fermt2重組蛋白
貨號：

CSB-BP809298MO
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Fermt2重組蛋白
貨號：

CSB-MP809298MO
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Fermt2
Uniprot No.：

Q8CIB5
別名：

Fermt2; Plekhc1Fermitin family homolog 2; Kindlin-2; Pleckstrin homology domain-containing family C member 1
種屬：

Mus musculus (Mouse)
蛋白長度：

full length protein
表達區域：

1-680
氨基酸序列

MALDGIRMPD GCYADGTWEL SVHVTDLNRD VTLRVTGEVH IGGVMLKLVE KLDVKKDWSD HALWWEKKRT WLLKTHWTLD KCGIQADAKL QFTPQHKLLR LQLPNMKYVK VKVNFSDRVF KAVSDICKTF NIRHPEELSL LKKPRDPTKK KKKKLDDQSE DEALELEGPL IMPGSGSIYS SPGLYSKTMT PTYDAHDGSP LSPTSAWFGD SALSEGNPGI LAVSQPVTSP EILAKMFKPQ ALLDKAKTNQ GWLDSSRSLM EQDVKENEAL LLRFKYYSFF DLNPKYDAIR INQLYEQAKW ALLLEEIECT EEEMMMFAAL QYHINKLSIM TSENHLNNSD KEVDEVDAAL SDLEITLEGG KTSTILGDIT SIPELADYIK VFKPKKLTLK GYKQYWCTFK DTSISCYKSR EESSGTPAHQ LNLRGCEVTP DVNISGQKFN IKLLIPVAEG MNEIWLRCDN EKQYAHWMAA CRLASKGKTM ADSSYNLEVQ NILSFLKMQH LNPDPQLIPD QITTDVNPEC LVSPRYLKKY KSKQITARIL EAHQNVAQMS LIEAKMRFIQ AWQSLPEFGI THFIARFQGG KREELIGIAY NRLIRMDAST GDAIKTWRFS NMKQWNVNWE IKMVTVEFAD EVRLSFICTE VDCKVVHEFI GGYIFLSTRA KDQNESLDEE MFYKLTSGWV
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Scaffolding protein that enhances integrin activation mediated by TLN1 and/or TLN2, but activates integrins only weakly by itself. Binds to membranes enriched in phosphoinositides. Enhances integrin-mediated cell adhesion onto the extracellular matrix and cell spreading; this requires both its ability to interact with integrins and with phospholipid membranes. Required for the assembly of focal adhesions. Participates in the connection between extracellular matrix adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits FBLIM1 to focal adhesions. Plays a role in the TGFB1 and integrin signaling pathways. Stabilizes active CTNNB1 and plays a role in the regulation of transcription mediated by CTNNB1 and TCF7L2/TCF4 and in Wnt signaling.
基因功能參考文獻：
1. In a mouse model of wound healing, KINDLIN-2 gene knockdown inhibited wound healing, and increased neovascular permeability in vivo. PMID: 30070977
2. Our studies identify Kindlin-2 as a stabilizing linker of endothelial AJs with the actin cytoskeleton, thereby re-enforcing the vascular barrier. PMID: 28799653
3. Kindlin-2 is a key protein that couples cell adhesion by activating integrins and the induction of membrane protrusions by activating Rac1 and supplying Rac1 with the Arp2/3 complex. PMID: 28912124
4. Postnatal loss of Kindlin-2 from cardiac myocytes leads to progressive heart failure. PMID: 27502369
5. Data indicate that Kindlin-2 not only maintains the cardiac structure but also is required for cardiac function. PMID: 27722852
6. These findings show that talin and kindlin cooperatively activate integrins leading to fibronectin binding and adhesion. PMID: 26821125
7. These studies uncover a previously unrecognized function for Kindlin-2 and a mechanism for regulation of the chondrocyte differentiation programme and chondrogenesis. PMID: 26151572
8. Taken together, our study suggests the suppressive roles of Kindlin-2 in the pathogenesis of colorectal carcinoma PMID: 25633062
9. Data suggest that kindlin-2 (Kind2/Fermt2) interacts with actin alpha 2 (Actn2) and integrin beta 1 (Itgb1) and co-localizes to cardiac sarcomere at Z-disc; knockdown of Kind2 leads to dissociation of Actn2 and Itgb1. PMID: 26143257
10. beta3/kindlin-2 interaction promotes outside-in alphaVbeta3 signaling selectively, with biological consequences in vivo [kindlin-2] PMID: 25587038
11. kindlin-2 tyrosine phosphorylation and interaction with Src serve as a regulatable switch downstream of focal adhesion kinase in the integrin outside-in signaling circuit controlling cell migration and proliferation PMID: 25237194
12. kindlin-2, through activating Ras and the downstream ERK1/2 and Akt signaling pathways, plays an important role in regulating renal tubular epithelial-to-mesenchymal transition PMID: 24226523
13. kindlin-2 regulates trafficking of endothelial cell surface enzymes that control platelet responses and hemostasis. PMID: 23896409
14. Kindlin-2 mediates activation of TGF-beta/Smad signaling and renal fibrosis. PMID: 23723426
15. Kindlin has a role in regulating integrin alpha5beta1 surface expression. PMID: 22795696
16. Mediation of Rac1 activation by kindlin-2: an essential function in osteoblast adhesion, spreading, and proliferation PMID: 21590706
17. Endothelial cells derived from kindlin-2(+/-) mice had integrin expression levels similar to WT mice but reduced alphaVbeta3-dependent signaling, migration, adhesion, spreading, and tube formation. PMID: 21378273
18. physiological role for kindlin-2 in skin fibroblasts under normal steady-state conditions and during tissue regeneration PMID: 20861856
19. kindlin-2 is an essential component of the intercalated disc, is necessary for cytoskeletal organization at sites of membrane attachment, and is required for vertebrate myocardial formation and function PMID: 18174465
20. Kindlin-2 is a novel and essential element of bidirectional integrin signaling. PMID: 18483218
21. kindlin-2 is a novel integrin adaptor protein important for muscle differentiation, and is a critical regulator of myocyte elongation. PMID: 18611274
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亞細胞定位：

Cytoplasm. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, stress fiber. Cell junction, focal adhesion. Membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus. Cytoplasm, myofibril, sarcomere, I band. Cell surface.
蛋白家族：

Kindlin family
組織特異性：

Detected in adult heart muscle (at protein level). Detected in heart, skeletal muscle and testis.
數據庫鏈接：

KEGG: mmu:218952

STRING: 10090.ENSMUSP00000044554

UniGene: Mm.210018