Recombinant Mouse Eukaryotic translation initiation factor 2-alpha kinase 4 (Eif2ak4), partial
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中文名稱：小鼠Eif2ak4重組蛋白
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貨號：CSB-EP879465MO-B
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說明書：
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規(guī)格：
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來源：E.coli
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共軛：Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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其他：
產(chǎn)品詳情
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純度：>85% (SDS-PAGE)
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基因名：
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Uniprot No.：
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別名：Eif2ak4; Gcn2; Kiaa1338eIF-2-alpha kinase GCN2; Eukaryotic translation initiation factor 2-alpha kinase 4; EC 2.7.11.1; GCN2-like protein; mGCN2
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種屬：Mus musculus (Mouse)
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蛋白長度：Partial
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蛋白標簽：Tag?type?will?be?determined?during?the?manufacturing?process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially. -
產(chǎn)品提供形式：Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
復(fù)溶：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
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儲存條件：Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質(zhì)期：The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期：Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
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注意事項：Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Datasheet ：Please contact us to get it.
產(chǎn)品評價
靶點詳情
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功能：Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to low amino acid availability. Plays a role as an activator of the integrated stress response (ISR) required for adaptation to amino acid starvation. EIF2S1/eIF-2-alpha phosphorylation in response to stress converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, leading to a global attenuation of cap-dependent translation, and thus to a reduced overall utilization of amino acids, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Binds uncharged tRNAs. Involved in cell cycle arrest by promoting cyclin D1 mRNA translation repression after the unfolded protein response pathway (UPR) activation or cell cycle inhibitor CDKN1A/p21 mRNA translation activation in response to amino acid deprivation. Plays a role in the consolidation of synaptic plasticity, learning as well as formation of long-term memory. Plays a role in neurite outgrowth inhibition. Plays a role in feeding behavior to maintain amino acid homeostasis; contributes to the innate aversion toward diets of imbalanced amino acid composition. Plays a proapoptotic role in response to glucose deprivation. Promotes global cellular protein synthesis repression in response to UV irradiation independently of the stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) and p38 MAPK signaling pathways.; (Microbial infection) Plays a role in the antiviral response against alphavirus infection; impairs early viral mRNA translation of the incoming genomic virus RNA, thus preventing alphavirus replication.; (Microbial infection) Plays a role in modulating the adaptive immune response to Yellow fever virus infection; promotes dendritic cells to initiate autophagy and antigene presentation to both CD4(+) and CD8(+) T-cells under amino acid starvation.
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基因功能參考文獻：
- our data provide solid evidence that GCN2 has a marked effect on Dox induced myocardial apoptosis and oxidative stress. Our findings suggest that strategies to inhibit GCN2 activity in cardiomyocyte may provide a novel approach to attenuate Dox-related cardiotoxicity. PMID: 29660505
- heme-regulated inhibitor (HRI), protein kinase R (PKR), PKR-like endoplasmic reticulum kinase, (PERK) and general control non-depressible 2 (GCN2) are sufficient for the integrated stress response; there are no additional eIF2alpha kinases in vertebrates PMID: 27633668
- These data point to a novel role for GCN2 and phosphorylation of eIF2alpha in the control of mTORC1 by certain amino acids. PMID: 27297692
- We hypothesize that the essential role of methionine-charged initiator tRNA in forming ternary complex is responsible for the robust ability of methionine deficiency to induce ATF4 and the ISR even in the absence of GCN2 or eIF2alpha kinase activity. PMID: 27613409
- GCN2 is only transiently required for LP-induced FGF21. PMID: 27396336
- GCN2 is required for normal cytotoxic T cell function. PMID: 27880901
- The data of this study suggest an important contribution of the General control non-derepressible 2 (GCN2) in T cell stress response to the resolution of autoimmune neuroinflammation. PMID: 27397084
- GCN2 activation increased phosphorylation of its substrate eIF2alpha and the induction of the integrated stress response master regulator, ATF4. PMID: 27852836
- GCN2 predisposes the exocrine pancreas to a maladaptive ER stress response and autophagy during asparaginase treatment. PMID: 26968207
- Inhibition of eIF2alpha phosphorylation via PERK suppression and reversal of de novo protein synthesis deficits can mitigate cognitive deficits in neurodegenerative diseases. PMID: 27103515
- Dietary EAA sensing depends on physiologic need, but not GCN2. PMID: 26526991
- Targeting ALDH18A1 activated the serine/threonine protein kinase GCN2 (general control nonderepressible 2) to inhibit protein synthesis in melanoma. PMID: 26082174
- acute amino acid starvation suppressed intestinal inflammation via a mechanism dependent on GCN2; hence, results reveal a mechanism that couples amino acid sensing with control of intestinal inflammation via GCN2 PMID: 26982722
- Gcn2 & Atf4 are involved in L-proline metabolism regulation in embryonic stem cells. PMID: 25857264
- Metabolic stress signals delivered by GCN2 inhibit innate inflammatory responses to apoptotic cells, restricting adaptive immunity and preventing autoimmunity. GCN2 activation by halofunginone halobromide potently reduced autoimmune reactivity. PMID: 26261340
- p58IPK is a general inhibitor of the eIF2alpha kinases in that it also interacts with GCN2. Thus forced overexpression of cytoplasmic p58 delays eIF2alpha phosphorylation, suppresses GCN2 phosphorylation and prolongs protein synthesis PMID: 25329545
- Data indicate that the crystal structures of murine and yeast GCN2 protein kinase C-terminal domain (CTD) showed dimers. PMID: 24719324
- The results suggest that the GCN2 kinase-dependent sensing of indoleamine 2,3-dioxygenase activity represents an important trigger to the experimental autoimmune encephalomyelitis remission phase. PMID: 24362236
- Deletion of the eIF2alpha Kinase GCN2 fails to rescue the memory decline associated with Alzheimer's disease. PMID: 24146979
- The present study provides the first direct evidence that inhibition of GCN2 activity by GCN2 gene deletion attenuates the development of heart failure. PMID: 24166753
- Data indicate that the GCN2 knockout mice showed reduced inflammatory responses, with decreased IL-6 and IL-12 expression correlating with significant reduction in animal mortality. PMID: 24248597
- This study extends our understanding of the roles played by GCN1 in GCN2 activation induced by a variety of stress arrangements and suggests that IMPACT and YIH1 use similar mechanisms for regulating this eIF2alpha kinase. PMID: 24333428
- GCN2 protects the brain from developing a lethal leukodystrophy in response to amino acid deficiencies. PMID: 24019515
- Data from knockout mice suggest that GCN2 (general control nondepressible-2) is important in maintaining gluconeogenesis in the liver; this effect is likely mediated through regulation of expression of Cebpb (CCAAT enhancer binding protein beta). PMID: 23900421
- Many genes with important functions in protein synthesis/degradation, transcriptional regulation and apoptosis were induced by NaSal and most of these were dependent on GCN2. PMID: 23356852
- the participation of the GCN2-IMPACT module of translational regulation in a highly controlled step in the development of the nervous system. PMID: 23447528
- GCN2 kinase is a key regulator of fibrogenesis and acute and chronic liver injury induced by carbon tetrachloride in mice. PMID: 23318883
- Both dietary and pharmacological interventions required the amino acid sensor and eIF2alpha kinase Gcn2, implicating the amino acid starvation response and translational control in stress protection. PMID: 22277968
- High dosage of resveratrol pretreatment inhibited the injury-induced up-regulation of eIF2alpha-CHOP and IRE1alpha-XBP1 pathways. PMID: 22491413
- Data from GCN2 knockout mice suggest that GCN2 participates in the control of food preferences; studies address role of GCN2 in macronutrient selection and its age-related changes. PMID: 21614613
- describe the recessive, loss-of-function mutation atchoum (atc) in Eif2ak4, encoding GCN2, which increased susceptibility to infection by the double-stranded DNA viruses mouse cytomegalovirus (MCMV) and human adenovirus PMID: 22114338
- It was shown that urea-inducible GCN2 activation initiates the phosphorylation of eIF2alpha and the downstream increase of activating transcription factor 3. PMID: 21880833
- GCN2 and its downstream signaling pathway have an important role in the protection against oxidative injuries induced by an amino acid-imbalanced diet, and that it can play a critical role in the prevention of oxidative damage PMID: 22020073
- PERK and GCN2 also have a functional role on regulating translation under non-stressed conditions, apart from their long established roles as stress kinases. PMID: 21638082
- amino acid starvation was able to induce p8 expression; GCN2/ATF4 pathway is involved in this regulation through the presence of an Amino Acid Response Element in p8 promoter. PMID: 21867687
- the GCN2/eIF2alpha/ATF4 pathway is essential for the induction of the TRB3 gene transcription PMID: 21203563
- the effects of arginine deficiency were solely mediated by GCN2 PMID: 21239484
- The eIF2alpha kinases PKR and GCN2 promote apoptosis. PMID: 21076179
- Results from knockout mice emphasize importance of GCN2 in adaptation of immune system in amino acid starvation/deprivation. PMID: 20861212
- Data show that reduced phosphorylation of eIF2alpha by knocking out either PERK or GCN2 suppresses hypoxia-induced G(1) arrest and promotes apoptosis in accompany with activation of p53 signal cascade. PMID: 20072654
- Data demonstrated that eIF2alpha kinases GCN2 and PERK differentially regulate mRNA transcription and translation, which in the latter case suggests that increased eIF2alpha phosphorylation is not sufficient for derepression of translation. PMID: 19509078
- regulated translation initiation controls stress-induced gene expression in mammalian cells PMID: 11106749
- activation in UV-irradiated cells inhibits translation PMID: 12176355
- GCN2 has a role in maintaining protein mass in skeletal muscle and liver PMID: 15213227
- reported that the mechanism for recognizing indispensable amino acid-deficient foods follows the conserved general control system, wherein uncharged transfer RNA induces phosphorylation of eukaryotic initiation factor 2 alpha via the GCN2 kinase PMID: 15774759
- GCN2 acts as a molecular sensor in T cells, allowing them to detect and respond to conditions created by indoleamine 2,3-dioxygenase. PMID: 15894280
- Overexpression of IMPACT in mouse embryonic fibroblasts inhibited phosphorylation of eIF2alpha by GCN2. PMID: 15937339
- Brain-specific inactivation of GCN2 impairs the aversive response of omnivores to otherwise nutritious foods of imbalanced amino acid content. PMID: 16054071
- genetic, physiological, behavioural and molecular evidence that GCN2 regulates synaptic plasticity, as well as learning and memory, through modulation of the ATF4/CREB pathway PMID: 16121183
- functions to cooperatively regulate eIF2alpha phosphorylation and cyclin D1 translation after unfolded response pathway activation. PMID: 16176978
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亞細胞定位：Cytoplasm.
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蛋白家族：Protein kinase superfamily, Ser/Thr protein kinase family, GCN2 subfamily
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組織特異性：Expressed in liver. Expressed predominantly in the hippocampal CA1 region and the dentate gyrus, and to a lesser degree in CA3 (at protein level). Expressed in liver, lung, brain, kidney, skeletal muscle and testis. Expressed weakly in heart and spleen. E
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