Recombinant Mouse EGF-containing fibulin-like extracellular matrix protein 1 (Efemp1)

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中文名稱：

小鼠Efemp1重組蛋白
貨號：

CSB-YP007450MO
說明書：

MSDS Datasheet
規(guī)格：

￥1836
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
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靶點詳情

產(chǎn)品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

Efemp1
Uniprot No.：

Q8BPB5
別名：

Efemp1; Fbln3EGF-containing fibulin-like extracellular matrix protein 1; Fibulin-3; FIBL-3
種屬：

Mus musculus (Mouse)
蛋白長度：

Full Length of Mature Protein
來源：

Yeast
分子量：

55.0kDa
表達區(qū)域：

18-493aa
氨基酸序列

QYTEETITYTQCTDGYEWDPIRQQCKDIDECDIVPDACKGGMKCVNHYGGYLCLPKTAQIIVNNEHPQQETPAAEASSGATTGTVAARSMATSGVVPGGGFMASATAVAGPEVQTGRNNFVIRRNPADPQRIPSNPSHRIQCAAGYEQSEHNVCQDIDECTSGTHNCRTDQVCINLRGSFTCQCLPGYQKRGEQCVDIDECTVPPYCHQRCVNTPGSFYCQCSPGFQLAANNYTCVDINECDASNQCAQQCYNILGSFICQCNQGYELSSDRLNCEDIDECRTSSYLCQYQCVNEPGKFSCMCPQGYEVVRSRTCQDINECETTNECREDEMCWNYHGGFRCYPRNPCQDHYVLTSENRCVCPVSNTMCRELPQSIVYKYMSIRSDRSVPSDIFQIQATMIYANTINTFRIKSGNENGEFYLRQTSPVSAMLVLVKSLSGPREYIVDLEMLTVSSIGTFRTSSVLRLTIIVGPFSF
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評價

問答及客戶評論

■ 常見問題解答

Is it possible to use Efemp1 to coat cell culture dishes?

Thanks for your inquiry. Do you plan to perform protein function related experiments? We haven't tested the activity of this protein so we can't 100% guarantee that it is active. However the protein we provide is full length of mature protein and purified under mild conditions, which should have activity in theory. If you plan to perform cell experiment, we suggest to process the protein with endotoxin removal and aseptic processing. The endotoxin level will be less than 1.0 EU per 1μg of the protein by the LAL method.

Can it be used to coat cell culture dishes?

Thanks for your inquiry. You could try to coat cell culture dishes but we can't guarantee the activity of the protein.

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靶點詳情

功能：

Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth.
基因功能參考文獻：
1. TWEAK promotes migration and invasion in murine embryonic fibroblasts through a mechanism dependent on ERKs activation and Fibulin 3 down-regulation. PMID: 28383766
2. Mutant fibulin-3 causes proteoglycan accumulation, reduction of MMP-2 and MMP-9, but increase of TIMP-3, and impairs diffusion across Bruch's membrane. PMID: 28622396
3. Define the Efemp1-p27(Kip1) axis as a potential marker module of PDAC cell sensitivity towards dual PI3K-mTOR inhibitors. PMID: 23470560
4. The Efemp1 knock-in mice reconstitute the most important histopathologic symptoms of both Malattia leventinese and age-related macular degeneration. PMID: 17664227
5. fibulin-3 plays an important role in maintaining the integrity of fascia connective tissues and regulates aging. PMID: 17872905
6. both fibulin-3 and -5 are important in maintaining pelvic organ support in mice. PMID: 19095964
7. Data suggest a role for fibulin-3 in tissue maintenance and regeneration in the adult olfactory pathway. PMID: 19520159
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亞細胞定位：

Secreted, extracellular space. Secreted, extracellular space, extracellular matrix.
蛋白家族：

Fibulin family
數(shù)據(jù)庫鏈接：

KEGG: mmu:216616

STRING: 10090.ENSMUSP00000020759

UniGene: Mm.44176