Recombinant Mouse E3 ubiquitin-protein ligase rififylin (Rffl)

中文名稱：

小鼠Rffl重組蛋白
貨號：

CSB-YP751168MO
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

小鼠Rffl重組蛋白
貨號：

CSB-EP751168MO
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

小鼠Rffl重組蛋白
貨號：

CSB-EP751168MO-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Rffl重組蛋白
貨號：

CSB-BP751168MO
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Rffl重組蛋白
貨號：

CSB-MP751168MO
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Rffl
Uniprot No.：

Q6ZQM0
別名：

Rffl; E3 ubiquitin-protein ligase rififylin; EC 2.3.2.27; RING finger and FYVE-like domain-containing protein 1; Fring; RING-type E3 ubiquitin transferase rififylin
種屬：

Mus musculus (Mouse)
蛋白長度：

full length protein
表達區域：

1-377
氨基酸序列

MSQPSLWKDS HYFIMWASCC NWFCLDGQPE EAPPPQGART QAYSNPGYSS FPSPTGSEPS CKACGVHFAS TTRKQTCLDC KKNFCMTCSS QEGNGPRLCL LCLRFRATAF QREELMKMKV KDLRDYLSLH DISTEMCREK EELVFLVLGQ QPVISEADRT RVPHLPQAFP EQQAFLTQPQ TSTVPPTSPG LPSSPAQVTS VPLAQDQETQ QAVGHVSQDH EEPIFPESTA RVPTEDETQS VDSEDSFVPG RRASLSDLTH LEDIEGLTVR QLKEILARNF VNYKGCCEKW ELMERVTRLY KDQKGLQHLV SGNEDQNGGA VPSGLEENLC KICMDSPIDC VLLECGHMVT CTKCGKRMNE CPICRQYVIR AVHVFRS
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

E3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Mediates 'Lys-48'-linked polyubiquitination of PRR5L and its subsequent proteasomal degradation thereby indirectly regulating cell migration through the mTORC2 complex. Also ubiquitinates the caspases CASP8 and CASP10, promoting their proteasomal degradation, to negatively regulate apoptosis downstream of death domain receptors. Also negatively regulates the tumor necrosis factor-mediated signaling pathway through targeting of RIPK1 to ubiquitin-mediated proteasomal degradation. Negatively regulates p53/TP53 through its direct ubiquitination and targeting to proteasomal degradation. Indirectly, may also negatively regulate p53/TP53 through ubiquitination and degradation of SFN. May also play a role in endocytic recycling.
基因功能參考文獻：
1. EGF, which signals through CRAF, and an activated BRAF mutant also activate PKC and stimulate cell migration through up-regulating RFFL expression. PMID: 24114843
2. Data show that the the amino-terminal domain of rififylin is critical for its recruitment to recycling endocytic membranes and for the inhibition of recycling. PMID: 15229288
3. CARP2 [Rififylin] deficiency does not alter induction of NF-kappaB by TNFalpha. PMID: 19138581
亞細胞定位：

Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein. Recycling endosome membrane; Peripheral membrane protein. Note=The FYVE-type zinc finger may mediate phosphatidylinositol phosphate-binding and control subcellular localization.
組織特異性：

Ubiquitous. Detected in heart, brain, spleen, lung, liver, skeletal muscle, kidney, testis, thymus, whole embryo and embryonic stem cells.
數據庫鏈接：

KEGG: mmu:67338

STRING: 10090.ENSMUSP00000071150

UniGene: Mm.341608