Recombinant Mouse E3 ubiquitin-protein ligase FANCL (Fancl)

中文名稱：

小鼠Fancl重組蛋白
貨號：

CSB-YP871894MO
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

小鼠Fancl重組蛋白
貨號：

CSB-EP871894MO
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

小鼠Fancl重組蛋白
貨號：

CSB-EP871894MO-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Fancl重組蛋白
貨號：

CSB-BP871894MO
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Fancl重組蛋白
貨號：

CSB-MP871894MO
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Fancl
Uniprot No.：

Q9CR14
別名：

Fancl; Phf9; PogE3 ubiquitin-protein ligase FANCL; EC 2.3.2.27; Fanconi anemia group L protein homolog; Proliferation of germ cells protein; RING-type E3 ubiquitin transferase FANCL
種屬：

Mus musculus (Mouse)
蛋白長度：

Full Length of Mature Protein
表達區域：

2-375
氨基酸序列

DEAEASLLR HFPLLLPQNR EKTVYEGFIS AQGSDFHLRI VLPKDLQLKK ARLLCSLQLK NILNEYHQVV QQRMKHSPDL MSFMMELKMI LEVALKNKQE LCVQPPSCSF CKDLLTEIGA IGWDKLACVE SSFSTIKLKA DDASGRKHLI TVKLKAKYPV EPPDCVVDFP VPFSVSWTPQ SSLVDVYSQF LVALETLKVF WDVMDEIDEK TWVLEPEKPP RSATARRIAL GKNVSIAIEV DPRHPTMLPE FCFLGADHVT KPLGMKLSGS IHLWDPENSL LQNLKDVLEI DFPARSILEE SDFSMDCGIC YARHLNGAIP DQVCNNPQCG QPFHEICLYE WLRGLSTSRQ SFNVFFGDCP YCSKPITLKM SGRKP
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Ubiquitin ligase protein that mediates monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCI. May stimulate the ubiquitin release from UBE2W. May be required for proper primordial germ cell proliferation in the embryonic stage, whereas it is probably not needed for spermatogonial proliferation after birth.
基因功能參考文獻：
1. The authors report the structure of the human FANCL central domain and show that the RING domain is necessary and sufficient for E2 binding. PMID: 21775430
2. Using HeLa cells and lymphoblasts from Fanconi anemia patients, the authors characterize PHF9 (FANCL) as a ubiquitin ligase and show it is essential for FANCD2 monoubiquitination. The authors state that PHF9 is the human homolog of mouse Pog (Fancl). PMID: 1297335
3. FANCL increases the activity and expression of beta-catenin, a key pluripotency factor in hematopoietic stem cells. PMID: 22653977
4. Fancl is associated with acute lung injury PMID: 21297076
5. expression of a novel splice variant of FA complementation group L (FANCL), named FAVL, can impair the FA pathway in non-FA tumor cells and act as a tumor promoting factor PMID: 20407210
6. Deletion of Pog causes germ cell deficiency, but is also accompanied by reduced embryonic body weight and sometimes lethality. PMID: 12417526
7. GGN1 and GGN3, interacted with POG, regulated the localization of POG, and played a role in spermatogenesis. PMID: 12574169
8. unlike in POG-deficient females, the germ cells in POG-deficient males eventually populate the seminiferous tubules at 9 wk, and fertility can be achieved by 12 wk. PMID: 12606378
9. Germ cell depletion 2 (gcd2) is a chemically induced recessive mutation that causes infertility in male and female mice. PMID: 16822657
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相關疾病：

Defects in Fancl are a cause of the gcd (germ cell deficient) mutant phenotype, which leads to reduced numbers of precursor germ cells and adult sterility, probably due to a reduced precursor germ cells proliferation.
亞細胞定位：

Cytoplasm. Nucleus. Note=In the nucleus, colocalizes with UBE2W.
數據庫鏈接：

KEGG: mmu:67030

STRING: 10090.ENSMUSP00000004120

UniGene: Mm.18875