Recombinant Mouse Double-stranded RNA-specific adenosine deaminase (Adar), partial

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中文名稱：

Recombinant Mouse Double-stranded RNA-specific adenosine deaminase (Adar), partial
貨號：

CSB-EP859990MO
說明書：

MSDS Datasheet
規(guī)格：

￥1836
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

Greater than 95% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

Adar
Uniprot No.：

Q99MU3
別名：

DRADA;EC 3.5.4.37;RNA adenosine deaminase 1
種屬：

Mus musculus (Mouse)
蛋白長度：

Partial
來源：

E.coli
分子量：

44.9 kDa
表達(dá)區(qū)域：

835-1170aa
氨基酸序列

SLGTGNRCVKGDSLSLKGETVNDCHAEIISRRGFIRFLYSELMKYNHHTAKNSIFELARGGEKLQIKKTVSFHLYISTAPCGDGALFDKSCSDRAVESTESRHYPVFENPKQGKLRTKVENGEGTIPVESSDIVPTWDGIRLGERLRTMSCSDKILRWNVLGLQGALLTHFLQPVYLKSVTLGYLFSQGHLTRAICCRVTRDGKAFEDGLRYPFIVNHPKVGRVSVYDSKRQSGKTKETSVNWCMADGYDLEILDGTRGTVDGPGKELSRVSKKNIFLQFKKLCSFRARRDLLQLSYGEAKKAARDYDLAKNYFKKSLRDMGYGNWISKPQEEKNF
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

C-terminal 6xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評價

靶點(diǎn)詳情

功能：

Catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA) referred to as A-to-I RNA editing. This may affect gene expression and function in a number of ways that include mRNA translation by changing codons and hence the amino acid sequence of proteins; pre-mRNA splicing by altering splice site recognition sequences; RNA stability by changing sequences involved in nuclease recognition; genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication; and RNA structure-dependent activities such as microRNA production or targeting or protein-RNA interactions. Can edit both viral and cellular RNAs and can edit RNAs at multiple sites (hyper-editing) or at specific sites (site-specific editing). Its cellular RNA substrates include: bladder cancer-associated protein (BLCAP), neurotransmitter receptors for glutamate (GRIA2) and serotonin (HTR2C) and GABA receptor (GABRA3). Site-specific RNA editing of transcripts encoding these proteins results in amino acid substitutions which consequently alters their functional activities. Exhibits low-level editing at the GRIA2 Q/R site, but edits efficiently at the R/G site and HOTSPOT1. Does not affect polyomavirus replication but provides protection against virus-induced cytopathic effects. Essential for embryonic development and cell survival and plays a critical role in the maintenance of hematopoietic stem cells.
基因功能參考文獻(xiàn)：
1. ADAR1 silencing reduces engraftment of myeloma in vivo. PMID: 29203771
2. ADAR1 functions other than RNA editing are reviewed. PMID: 28960389
3. protein recoding arising from ADAR1-mediated editing is not essential for organismal homeostasis PMID: 28874170
4. These findings shed a new light on the vital regulatory role of ADAR1(Adenosine deaminase) in hepatic immune homeostasis, chiefly its inhibitory function on the crosstalk between the NFkappaB and type-I interferons signaling cascades, restraining the development of liver inflammation and fibrosis. PMID: 27362366
5. ADAR1-mediated RNA editing is essential for normal erythropoiesis. PMID: 27373493
6. Knockout of ADAR1 dramatically inhibited injury-induced neointima formation and restored vascular smooth muscle cell remodeling. PMID: 27199464
7. we identified a novel ADAR1-dependent protective contribution through which hepatocytes guard against aberrant cytosolic RLR-RNA-sensing pathway mediated inflammatory reaction in response to acute liver IR. PMID: 26832817
8. Thus, Adarb1 and Adarb2 have nearly exclusive expression within brain tissue, while Adar has more appreciable expression across multiple tissues. PMID: 26768488
9. After fear conditioning protocol, mRNA expression of ADAR1 increased in amygdala and hippocampus. PMID: 26383032
10. ADAR1 is an essential molecule for maintaining adult liver homeostasis and, in turn, morphological and functional integrity. PMID: 26453800
11. The p150 isoform of ADAR1 uniquely regulated the MDA5 pathway. PMID: 26588779
12. A-to-I editing of endogenous dsRNA is the essential function of ADAR1, preventing the activation of the cytosolic dsRNA response by endogenous transcripts. PMID: 26275108
13. ADAR1 mutations causing Aicardi-Goutieres syndrome affect the activity of the interferon-inducible cytoplasmic isoform more severely than the nuclear isoform. PMID: 25456137
14. Analysis of editing in the filamin A encoding mRNA shows very high editing levels outside the nervous system; further shows FLNA editing is mainly achieved by ADAR2 but that in some cases ADAR1 can efficiently compensate for ADAR2. PMID: 24025532
15. Adenosine deaminase acting on RNA 1 limits RIG-I RNA detection and suppresses IFN production responding to viral and endogenous RNAs. PMID: 25172485
16. Findings highlight the fact that the differentially expressed ADARs in tumours, which are responsible for an A to I RNA editing imbalance, have great prognostic value and diagnostic potential for hepatocellular carcinoma. PMID: 23766440
17. Loss of ADAR1 induced ER stress and activation of IFN signaling, and altered expression in WNT targets, followed by intestinal inflammation. PMID: 23598411
18. ADAR1 differentiates its functions in RNA editing and RNAi by the formation of either ADAR1/ADAR1 homodimer or Dicer/ADAR1 heterodimer complexes, respectively. PMID: 23622242
19. results uncover a novel requirement for ADAR1 in myeloid leukemic cells and indicate that ADAR1 may comprise a new molecular target for CML-directed therapeutics PMID: 22987615
20. ADAR1 induced adenosine-targeted DNA mutations in IgM B cells from ADAR1-transgenic mice and in wild-type mouse embryonic fibroblasts PMID: 23209284
21. Analysis of the results identified ADAR1 as a new key regulator of bone mass and suggested that ADAR1 functions in this process mainly through modulation of the intrinsic properties of osteoblasts. PMID: 23123729
22. miRNA abundance and miRNA processing are mainly influenced by ADARB1; an additional loss of ADAR has only a minor effect on expression of mature miRNAs. PMID: 22310477
23. mRNA expressions of P110 and P150 ADAR1 isoforms show diverse patterns in the development of leukemia, suggesting that RNA editing mediated by ADAR1 isoforms may play different roles in leukemia. PMID: 21729523
24. Our results suggest that ADAR1 impairs cell-destructive processes following polyomavirus infection. PMID: 21632755
25. Data show that that the P110 isoform is generated when knockout cells are reconstituted with a P150 cDNA expression vector. PMID: 21593418
26. ADAR1 is a restriction factor in the replication of paramyxoviruses and orthomyxoviruses. PMID: 21173229
27. These findings suggest that ADAR1 is essential for the maintenance of function of T lymphocytes during acute rejection. PMID: 19757169
28. These data identify a novel regulatory role for ADAR1 activation under inflammatory stress to both promote muscle protein synthesis pathways and limit atrophy pathways. PMID: 20590675
29. Activation of phospholipase C and alternative splicing of pre-mRNA encoding the editing enzymes ADAR1 and ADAR2 have both overlapping and specific roles in modulating 5-HT2CR editing phenotypes. PMID: 20651031
30. ADAR1 has a role in protein translation independent of RNA editing PMID: 12453429
31. ADAR1 variants are differentially regulated during acute inflammation: the localization of these variants and of A-to-I RNA editing in the cytoplasm, nucleus, and nucleolus is intracellularly reorganized in response to inflammatory stimulation PMID: 12954622
32. results demonstrate an essential requirement for ADAR1 in embryogenesis and suggest that it functions to promote survival of numerous tissues by editing one or more double-stranded RNAs required for protection against stress-induced apoptosis PMID: 14613934
33. ADAR1 selectively edits in vivo two of five closely spaced adenosines in the serotonin 5-hydroxytryptamine subtype 2C receptor pre-mRNA of nervous tissue; and hence, site-selective adenosine-to-inosine editing is indeed a function of ADAR1. PMID: 14615479
34. the intracellular distribution of the various ADAR1 isoforms is determined by NLS-c, NES, NoLS, and a regulatory motif PMID: 14711814
35. ADAR1 as a cellular factor that limits the efficacy of siRNA in mammalian cells PMID: 15556947
36. the IFN inducible promoter and exon 1A-containing RNA are primarily responsible for the increased ADAR1 observed in Salmonella-infected mice PMID: 15677478
37. The roles of meri-1 (mouse enhanced RNAi) and adar1 in the regulation of RNA interference are reported.[meri-1] PMID: 16004606
38. ADAR1 has the potential both to change information content through editing of mRNA and to regulate gene expression through interacting with NF90 PMID: 16055709
39. The level of Adar1 mRNA in NG108-15 cells was decreased by cell differentiation; this may play an important role in the differentiation-induced alteration of the 5-HT2CR editing pattern in this tumor cell line. PMID: 16082172
40. mature miRNA-142 expression levels increased substantially in ADAR1 null or ADAR2 null mice PMID: 16369484
41. Possible target preferences of ADAR1 and ADAR2 were examined by determining the editing status of transcripts derived from mice deficient in either enzyme. PMID: 18430892
42. ADAR1 is essential for the maintenance of hematopoiesis and suppression of interferon signaling. PMID: 19060901
43. ADAR1 and ADAR2 are broadly distributed in most regions of the mouse forebrain by P0. PMID: 19325227
44. Results show that show that this regulation of editing activity cannot be explained by an altered expression of the ADAR proteins but, rather, by the presence of a regulatory network that controls the editing activity during development. PMID: 19420382
45. ADAR1-deficient hematopoietic stem cells (HSCs) were incapable of reconstituting irradiated recipients although being phenotypically present in the recipient bone marrow. ADAR1 functions in HPCs via an RNA-editing dependent mechanism. PMID: 19805087
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亞細(xì)胞定位：

[Isoform 1]: Cytoplasm. Nucleus, nucleolus. Note=Long forms starting at Met-1 are found predominantly in cytoplasm. Shuttles between the cytoplasm and nucleus.; [Isoform 2]: Cytoplasm. Nucleus, nucleolus. Note=Long forms starting at Met-1 are found predominantly in cytoplasm.; [Isoform 3]: Nucleus, nucleolus. Note=Short forms starting at Met-519 are found exclusively in the nucleolus.; [Isoform 4]: Nucleus, nucleolus. Note=Short forms starting at Met-519 are found exclusively in the nucleolus.
組織特異性：

Highest levels in brain and spleen. Lowest levels in liver.
數(shù)據(jù)庫鏈接：

KEGG: mmu:56417

STRING: 10090.ENSMUSP00000103028

UniGene: Mm.316628