Recombinant Mouse C-type natriuretic peptide (Nppc)

中文名稱：

Recombinant Mouse C-type natriuretic peptide (Nppc)
品名簡稱：

Recombinant Mouse Nppc protein
貨號：

CSB-EP713991MO
說明書：

MSDS Datasheet
規格：

￥1536
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

Nppc
Uniprot No.：

Q61839
別名：

Nppc;Cnp
種屬：

Mus musculus (Mouse)
蛋白長度：

Full Length of Mature Protein
來源：

E.coli
分子量：

15.8 kDa
表達區域：

74-126aa
氨基酸序列

DLRVDTKSRAAWARLLHEHPNARKYKGGNKKGLSKGCFGLKLDRIGSMSGLGC
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged and C-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Hormone which plays a role in endochondral ossification through regulation of cartilaginous growth plate chondrocytes proliferation and differentiation. May also be vasoactive and natriuretic. Acts by specifically binding and stimulating NPR2 to produce cGMP. Binds the clearance receptor NPR3.
基因功能參考文獻：
1. this study shows that Nppc induces sperm attraction for fertilization PMID: 28054671
2. E2-ERs system was functional in maintaining oocyte meiotic arrest by regulating the expression of natriuretic peptide C and natriuretic peptide receptor 2 (NPPC/NPR2) PMID: 28277543
3. CNP activates bone turnover and remodeling in vivo and possibly accelerates fracture healing in a mouse model. PMID: 25860030
4. The up-to-day world-wide data about the structure, distribution, and physiological effects of the most poorly known among the natriuretic peptides--the C-type (CNP)--are summarized in the review. PMID: 26263678
5. Connexin mimetics directed against connexin-43 (Cx43 CMP) overcame NPPC-mediated meiotic arrest in both isolated cumulus oocyte complexes and antral follicles. PMID: 24804968
6. CNP/NPR-C pathway has potential as a disease-modifying therapeutic target for cardiovascular disorders PMID: 25105365
7. Data indicate an important, previously neglected, role of NEP for regulation of luminal factors in the epididymis and suggest a novel role for CNP/guanylyl cyclase B in the epididymal epithelium PMID: 24099862
8. CNP overexpression in chondrocytes can prevent endochondral growth delay and protect against cartilage damage in a mouse model of inflammatory arthritis. PMID: 24449577
9. Data suggest that intracerebroventricular administration of C-type natriuretic peptide (CNP-22 or CNP-53) has anorexigenic effect on hypothalamic neurons via activation of melanocortin/melanocortin receptor signal transduction. PMID: 23274904
10. CNP/NPR2 signaling is essential for oocyte meiotic arrest in early antral follicles and activated LH/amphiregulin/EGFR signaling pathway suppresses this signal by downregulating Nppc expression. PMID: 22987720
11. we show that CNP plays important region-specific roles in the GI tract of mice. PMID: 23127564
12. CNP secreted by growing follicles is capable of stimulating preantral and antral follicle growth PMID: 22595960
13. NPPC/NPR2 signaling is essential for oocyte meiotic arrest and cumulus oophorus formation, which affects female fertility through the production of oocytes with developmental capacity. PMID: 22696190
14. GTP-binding protein betagamma subunit-dependent potentiation of TRPV1 as novel signaling cascades recruited by CNP in dorsal root ganglia neurons leads to enhanced nociceptor excitability and thermal hypersensitivity and increases neuronal firing frequency. PMID: 22933780
15. These results demonstrate that lbab/lbab is a novel mouse model of chondrodysplasia caused by insufficient CNP action on endochondral ossification. PMID: 22271248
16. findings show mural granulosa cells express Nppc mRNA; cumulus cells surrounding oocytes express mRNA of NPPC receptor NPR2; granulosa cell ligand NPPC and receptor NPR2 in cumulus cells prevent precocious meiotic maturation PMID: 20947764
17. Data show that the mortality of CNP knockout mice was greatly decreased by circulating CNP. PMID: 20610569
18. CNP is a new regulator of food intake and energy expenditure. PMID: 20555027
19. Increased accumulation of CIOR in response to CNP might be related to the stimulation of osteoblastic mineralization by CNP. PMID: 11870417
20. study provides in vivo and in vitro genetic evidence that Cyclic GMP-dependent protein kinase II plays a critical role in CNP-mediated endochondral ossification PMID: 12193576
21. REVIEW: Significance of C-type natriuretic peptide (CNP) in endochondral ossification: analysis of CNP knockout mice. PMID: 12434160
22. Nppc is induced in renal tubular epithelia and then in interstitial myofibroblasts after unilateral ureteral obstruction and was activated in Wnt4-expressing cells during nephrogenesis PMID: 12475749
23. ANP has a role in vasodilating responsiveness to endothelial NO in mice PMID: 12637561
24. alterations in the renal CNP/NPR-B system may be an important physiological adjustment when water is scarce PMID: 14613785
25. C-type natriuretic peptide has a role in vasorelaxation in renal resistance arteries and aortas PMID: 15093694
26. Expression of CNP together with potent NPR-B receptors suggests that in podocytes, CNP may act in an autocrine and/or paracrine manner. PMID: 15327393
27. in hypertrophied mice, diminished effects of NO or CNP on ventricular myocyte contraction are not due to changes in guanylyl cyclase activity. PMID: 15550520
28. A single nucleotide mutation of Nppc is associated with dwarfism in lbab/lbab mice PMID: 17439653
29. Ostn is a naturally occurring ligand of the NPR-C clearance receptor and may act to locally modulate the actions of the natriuretic system in bone by blocking the clearance action of NPR-C, thus locally elevating levels of C-type natriuretic peptide. PMID: 17951249
30. Molecular modeling suggested that the conserved arginine is critical for binding to an equally conserved acidic pocket in NPR-B. These results indicate that reduced binding to and activation of NPR-B causes dwarfism in lbab(-/-) mice. PMID: 18554750
31. Results suggest that C-type natriuretic peptide is derived from Schwann cells and plays an important role for the development and function of nociceptive sensory neurones. PMID: 18752656
32. These results demonstrate that lbab/lbab mice have a hypomorphic mutation in the Nppc gene that is responsible for dwarfism caused by impaired endochondral ossification. PMID: 18775416
33. the secreted molecule C-type natriuretic peptide (CNP) induces a cGMP signaling cascade via its receptor particulate guanylyl cyclase Npr2 which is essential for sensory axon bifurcation at the dorsal root entry zone (DREZ) of the spinal cord PMID: 19805384
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亞細胞定位：

Secreted.
蛋白家族：

Natriuretic peptide family
數據庫鏈接：

KEGG: mmu:18159

STRING: 10090.ENSMUSP00000027449

UniGene: Mm.12802