Recombinant Mouse Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1 (Ndst1), partial

中文名稱：

小鼠Ndst1重組蛋白
貨號：

CSB-YP664945MO
說明書：

MSDS Datasheet
規(guī)格：
來源：

Yeast
其他：

中文名稱：

小鼠Ndst1重組蛋白
貨號：

CSB-EP664945MO
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
其他：

中文名稱：

小鼠Ndst1重組蛋白
貨號：

CSB-EP664945MO-B
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Ndst1重組蛋白
貨號：

CSB-BP664945MO
說明書：

MSDS Datasheet
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Ndst1重組蛋白
貨號：

CSB-MP664945MO
說明書：

MSDS Datasheet
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價
靶點詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

Ndst1
Uniprot No.：

Q3UHN9
別名：

Ndst1; Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1; EC 2.8.2.8; Glucosaminyl N-deacetylase/N-sulfotransferase 1; NDST-1; N-heparan sulfate sulfotransferase 1; N-HSST 1; [Heparan sulfate]-glucosamine N-sulfotransferase 1; HSNST 1) [Includes: Heparan sulfate N-deacetylase 1; EC 3.-.-.-); Heparan sulfate N-sulfotransferase 1; EC 2.8.2.-)]
種屬：

Mus musculus (Mouse)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Plays a role in determining the extent and pattern of sulfation of heparan sulfate. Compared to other NDST enzymes, its presence is absolutely required. Participates in biosynthesis of heparan sulfate that can ultimately serve as L-selectin ligands, thereby playing a role in inflammatory response. Required for the exosomal release of SDCBP, CD63 and syndecan.
基因功能參考文獻：
1. Podocyte-glomerular basement membrane interactions and cytoskeletal organization are disrupted in the glomeruli of the Ndst1-/- mutant mouse model PMID: 26936875
2. Although the renal phenotype of the Ndst1(-/-) mouse is mild, the data show that heparan chain N-sulfation plays a key role in podocyte organization. PMID: 23924956
3. Targeted ablation of Ndst1 in smooth muscle cells results in altered biomechanical properties of aorta. PMID: 24101444
4. Loss of NDST1 causes defective diaphragm vascular development and congenital diaphragmatic hernia. PMID: 24355925
5. The results of this study suggested that while Chst14 and its enzymatic products might be of limited importance for neural development, they may contribute to the regeneration-restricting environment in the adult mammalian nervous system PMID: 23360803
6. embryonic stem cells lacking both NDST1 and NDST2, expressing a very low sulfated heparan sulfate, can take the initial step toward differentiation into all three germ layers PMID: 22298785
7. Deletion of N-deacetylase-N-sulfotransferase1 in smooth muscle did not influence any of the blood pressure parameters measured despite significant decrease in aorta and thoracodorsal artery luminal area. PMID: 22555965
8. Lowered expression of NDST1 also results in a higher sulfate content of the heparin synthesized and is accompanied by increased levels of stored MC proteases. PMID: 22049073
9. Inhibitory peptides of the sulfotransferase domain of the heparan sulfate enzyme, N-deacetylase-N-sulfotransferase-1. PMID: 20129923
10. These data provide new evidence that modification of heparan sulfate fine structure through deletion of Ndst1 is sufficient to decrease vascular smooth muscle cell proliferation and alter vascular remodeling. PMID: 20206635
11. These findings demonstrate a selective, highly penetrant, cell autonomous effect of Ndst1-mediated sulfation on lobuloalveolar development. PMID: 20502530
12. Structural analyses of heparan sulfate produced by stable human embryonic 293 cells expressing mouse NDST-1 mutants indicate that the deacetylase of one enzyme and the sulfotransferase of another can act on the same glucosamine unit. PMID: 12590599
13. major changes in the heparan sulfate composition in skeletal muscle tissue derived from NDST-1-/- mice and NDST-/- cultured myotubes. PMID: 12692154
14. NDST-1 and C5-epimerase have roles in heparan sulfate structure alterations in mouse tissue PMID: 15292174
15. NDST1 and NDST2 have roles in N-sulfation but not in 6-O-sulfation of heparan sulfate PMID: 15319440
16. findings show that properly synthesized heparan sulfate is required for the normal development of the brain and face, and that Ndst1 is a modifier of heparan sulfate-dependent growth factor/morphogen signalling in those tissues PMID: 16020517
17. analysis of liver NDST1 and NDST2 biosynthesis PMID: 16984905
18. results establish an important role of Ndst1 function in FGF signaling during lens development PMID: 17107998
19. Heparan sulfate Ndst1 gene function variably regulates multiple signaling pathways during mouse development. PMID: 17183530
20. Reduction of N-sulfation due to deficiency in N-deacetylase/N-sulfotransferase-1 attenuated PDGF-BB binding in vitro, and led to pericyte detachment and delayed pericyte migration in vivo. PMID: 17289920
21. Ndst1 modulates BMPR and PTHrP signaling during endochondral bone formation in a gene knockout model. PMID: 17376755
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亞細胞定位：

Golgi apparatus membrane; Single-pass type II membrane protein.
蛋白家族：

Sulfotransferase 1 family, NDST subfamily
組織特異性：

Widely expressed in adult and throughout development.
數(shù)據(jù)庫鏈接：

KEGG: mmu:15531

STRING: 10090.ENSMUSP00000126623

UniGene: Mm.181862