Recombinant Japanese encephalitis virus Genome polyprotein, partial
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中文名稱：Recombinant Japanese encephalitis virus Genome polyprotein ,partial
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貨號：CSB-YP335779JAC
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說明書：
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規格：
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來源：Yeast
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其他：
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中文名稱：Recombinant Japanese encephalitis virus Genome polyprotein ,partial
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貨號：CSB-EP335779JAC
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說明書：
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規格：
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來源：E.coli
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其他：
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中文名稱：Recombinant Japanese encephalitis virus Genome polyprotein ,partial
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貨號：CSB-EP335779JAC-B
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說明書：
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規格：
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來源：E.coli
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共軛：Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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其他：
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中文名稱：Recombinant Japanese encephalitis virus Genome polyprotein ,partial
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貨號：CSB-BP335779JAC
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說明書：
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規格：
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來源：Baculovirus
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其他：
產品詳情
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純度：>85% (SDS-PAGE)
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基因名：N/A
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Uniprot No.：
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別名：Genome polyprotein [Cleaved into: Capsid protein C; Core protein); Protein prM; Peptide pr; Small envelope protein M; Matrix protein); Envelope protein E; Non-structural protein 1; NS1); Non-structural protein 2A; NS2A); Serine protease subunit NS2B; Flavivirin protease NS2B regulatory subunit; Non-structural protein 2B); Serine protease NS3; EC 3.4.21.91; EC 3.6.1.15; EC 3.6.4.13; Flavivirin protease NS3 catalytic subunit; Non-structural protein 3); Non-structural protein 4A; NS4A); Peptide 2k; Non-structural protein 4B; NS4B); RNA-directed RNA polymerase NS5; EC 2.1.1.56; EC 2.1.1.57; EC 2.7.7.48; Non-structural protein 5)]
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種屬：Japanese encephalitis virus (strain Jaoars982)
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蛋白長度：Partial
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蛋白標簽：Tag?type?will?be?determined?during?the?manufacturing?process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially. -
產品提供形式：Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
復溶：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
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儲存條件：Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質期：The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期：Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
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注意事項：Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Datasheet ：Please contact us to get it.
產品評價
靶點詳情
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功能：Plays a role in virus budding by binding to the cell membrane and gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle. During virus entry, may induce genome penetration into the host cytoplasm after hemifusion induced by the surface proteins. Can migrate to the cell nucleus where it modulates host functions. Overcomes the anti-viral effects of host EXOC1 by sequestering and degrading the latter through the proteasome degradation pathway.; Inhibits RNA silencing by interfering with host Dicer.; Prevents premature fusion activity of envelope proteins in trans-Golgi by binding to envelope protein E at pH 6.0. After virion release in extracellular space, gets dissociated from E dimers.; Acts as a chaperone for envelope protein E during intracellular virion assembly by masking and inactivating envelope protein E fusion peptide. prM is the only viral peptide matured by host furin in the trans-Golgi network probably to avoid catastrophic activation of the viral fusion activity in acidic Golgi compartment prior to virion release. prM-E cleavage is inefficient, and many virions are only partially matured. These uncleaved prM would play a role in immune evasion.; May play a role in virus budding. Exerts cytotoxic effects by activating a mitochondrial apoptotic pathway through M ectodomain. May display a viroporin activity.; Binds to host cell surface receptor and mediates fusion between viral and cellular membranes. Efficient virus attachment to cell is, at least in part, mediated by host HSPA5. Envelope protein is synthesized in the endoplasmic reticulum in the form of heterodimer with protein prM. They play a role in virion budding in the ER, and the newly formed immature particle is covered with 60 spikes composed of heterodimer between precursor prM and envelope protein E. The virion is transported to the Golgi apparatus where the low pH causes dissociation of PrM-E heterodimers and formation of E homodimers. prM-E cleavage is inefficient, and many virions are only partially matured. These uncleaved prM would play a role in immune evasion.; Involved in immune evasion, pathogenesis and viral replication. Once cleaved off the polyprotein, is targeted to three destinations: the viral replication cycle, the plasma membrane and the extracellular compartment. Essential for viral replication. Required for formation of the replication complex and recruitment of other non-structural proteins to the ER-derived membrane structures. Excreted as a hexameric lipoparticle that plays a role against host immune response. Antagonizing the complement function. Binds to the host macrophages and dendritic cells. Inhibits signal transduction originating from Toll-like receptor 3 (TLR3).; Component of the viral RNA replication complex that functions in virion assembly and antagonizes the host alpha/beta interferon antiviral response.; Required cofactor for the serine protease function of NS3. May have membrane-destabilizing activity and form viroporins.; Displays three enzymatic activities: serine protease, NTPase and RNA helicase. NS3 serine protease, in association with NS2B, performs its autocleavage and cleaves the polyprotein at dibasic sites in the cytoplasm: C-prM, NS2A-NS2B, NS2B-NS3, NS3-NS4A, NS4A-2K and NS4B-NS5. NS3 RNA helicase binds RNA and unwinds dsRNA in the 3' to 5' direction.; Regulates the ATPase activity of the NS3 helicase activity. NS4A allows NS3 helicase to conserve energy during unwinding.; Functions as a signal peptide for NS4B and is required for the interferon antagonism activity of the latter.; Induces the formation of ER-derived membrane vesicles where the viral replication takes place. Inhibits interferon (IFN)-induced host STAT1 phosphorylation and nuclear translocation, thereby preventing the establishment of cellular antiviral state by blocking the IFN-alpha/beta pathway. Inhibits STAT2 translocation in the nucleus after IFN-alpha treatment.; Replicates the viral (+) and (-) RNA genome, and performs the capping of genomes in the cytoplasm. NS5 methylates viral RNA cap at guanine N-7 and ribose 2'-O positions. Besides its role in RNA genome replication, also prevents the establishment of cellular antiviral state by blocking the interferon-alpha/beta (IFN-alpha/beta) signaling pathway. Inhibits host TYK2 and STAT2 phosphorylation, thereby preventing activation of JAK-STAT signaling pathway.
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基因功能參考文獻：
- The results indicated Hsp70 up-regulation by Japanese encephalitis virus NS5 not only involved in type I interferon antagonism, but also responded to the anti-apoptotic action of Japanese encephalitis virus NS5 protein through the blocking IFN-beta-induced p38 MAPK/STAT1-mediated apoptosis. PMID: 28559152
- the results demonstrate that SPCS1 affects viral replication by interacting with NS2B, thereby influencing the posttranslational processing of Japanese Encephalitis Virus proteins and the assembly of virions. PMID: 29593046
- a single serine-to-asparagine substitution [Ser(139)-->Asn(139) (S139N)] in the viral polyprotein substantially increased Zika Virus infectivity in both human and mouse neural progenitor cells and led to severe microcephaly PMID: 28971967
- Liposome and heparin binding assays indicate that only the N-terminal region of NS1 mediates interaction with membranes and that sulfate binding sites common to NS1 structures are not glycosaminoglycan binding interfaces. This report highlights several differences between flavivirus NS1 proteins and contributes to our understanding of their structure-pathogenic function relationships. PMID: 29343583
- Crystal structure of full-length Zika virus NS5 protein reveals a conformation similar to Japanese encephalitis virus NS5. PMID: 28291746
- The authors demonstrated that BST2 restricts the release of Japanese encephalitis virus whose budding occurs at the endoplasmic reticulum-Golgi intermediate compartment, and in turn, Japanese encephalitis virus downregulates BST2 expression via envelope protein E. PMID: 28710958
- These results suggest that the Japanese encephalitis virus NS5 inhibits the induction of type I interferon by targeting KPNA3 and KPNA4. PMID: 28179530
- results demonstrate that, in addition to being a cofactor for NS3 protease, flavivirus NS2B also functions in viral RNA replication, as well as virion assembly PMID: 27053551
- This study indicates that RNAi targeting of the NS3 and NS4A genes of JEV can sufficiently inhibit the replication of JEV in vitro and in vivo, and NS3 and NS4A genes might be potential targets of molecular therapy for JEV infection. PMID: 27593564
- Together, these results indicate that the introduction of a single mutation in the M protein, while being tolerated in insect cells, strongly impacts Japanese encephalitis virus infection in mammalian hosts. PMID: 26656690
- The high-mannose N-linked glycan at N154 of Japanese encephalitis virus E glycoprotein was shown to be crucial for binding to DC-SIGN and subsequent internalization. PMID: 26629951
- nonstructural protein 5 (NS5) interacted with hydroxyacyl-CoA dehydrogenase alpha and beta subunits, two components of the mitochondrial trifunctional protein (MTP) involved in LCFA beta-oxidation PMID: 25816318
- These results clearly showed the genetic interaction between NS4A and NS4B in virus replication, although the mechanism underlying their interaction is unknown. PMID: 25575708
- variations in Viral E glycoprotein determines neurovirulence. PMID: 25077483
- Together, these data showed that the methyltransferase-polymerase interface of NS5 is relevant in both polymerase initiation and elongation, likely with different regulation mechanisms in these two major phases of RNA synthesis. PMID: 25320292
- The linear epitope located in amino acids residues (2)(2)ETHTLW(2)(3)(2) was highly conserved among Japanese encephalitis virus strains. PMID: 24631788
- (394)HHWH(397)is the epitope of domain III of the envelope protein. PMID: 24184444
- Mutational analysis of key residues that interact with GTP evinces that the RNA-dependent RNA polymerase/GTP structure represents a novel pre-initiation state. PMID: 24293643
- Crystal Structure of the full-length Japanese encephalitis virus NS5 reveals a conserved methyltransferase-polymerase interface. PMID: 23950717
- These findings clearly indicate that NS1' enhances the production of Japanese encephalitis virus in avian cells and may facilitate the amplification/maintenance role of birds in the virus transmission cycle in nature. PMID: 24443559
- Japanese encephalitis virus nonstructural protein 2A blocks double-stranded RNA-activated protein kinase PKR PMID: 22787234
- A point mutation G66A in the NS2A gene abolished the production of NS1' in vitro and reduced neurovirulence. PMID: 22739060
- N-linked glycosylation in the prME and NS1 gene were correlated with the immunity. PMID: 22764522
- Amino acid residues located in the fusion loop of E domain II of E protein are critical for recognition by group cross-reactive mAbs. PMID: 22337639
- E protein assembles as an antiparallel dimer in the crystal lattice. PMID: 22156523
- The epitope located at aa 1-16,31-46, 131-146 and 301-316 of NS1 are conserved in West Nile virus and Japanese encephalitis virus. PMID: 21940411
- The comparison between the new genotype 1 JEV strains and the vaccine strain in E gene showed very high homology of base sequence and amino acid sequence. PMID: 22097600
- In addition to the core protein, hnRNP A2 also associated with Japanese encephalitis virus nonstructural protein 5 and with the 5'-untranslated region of the negative-sense Japanese encephalitis virus RNA. PMID: 21865391
- These data show that Tctex-1 may play a role in late stages of viral replication through its interaction with the flavivirus membrane protein. PMID: 21767858
- The NS3 protein was detectable 12 h post-infection and was distributed in the cytoplasm of infected cells. PMID: 21549011
- An amino acid substitution in NS4A protein increases its virulence in mice. PMID: 21411676
- novel mutation S227T in E gene loop region of domain II in virus circulating in North India PMID: 20727244
- These results suggest that CypB plays a crucial role in the replication of Japanese encephalitis virus through an interaction with NS4A. PMID: 21281954
- These results showed that JEV-MuLV pseudotyped viruses did not require prM for production of infectious pseudotyped viruses. PMID: 19640312
- results suggest that B23 plays an important role in the intracellular localization of the core protein and replication of Japanese encephalitis virus PMID: 16547420
- The results showed the capsid (core) protein had the ability to bind DNA and amino acids F46 to P61 mediate the DNA binding ability. PMID: 17957476
- a highly conserved N-glycosylation motif in prM is crucial for multiple stages of JEV biology: prM biogenesis, virus release, and pathogenesis PMID: 18524814
- JEV NS4A without the C-terminal 2K domain is associated with modulation of the IFN response and the interaction of JEV NS4A with RNA helicase DDX42 could be important for JE pathogenesis PMID: 18588927
- Motif of (146)EHARW(150) is the minimal unit of linear epitopes on NS1. PMID: 19185592
- NS1' may derive from ribosomal frameshifting as suggested by a conserved predicted pseudoknot in the NS2A-encoding sequence. [NS1', NS2A] PMID: 19196463
- JEV infection and the co-expression of JEV NS3 protease with NS2B cofactor induced caspase 3 activation and mitochondria-mediated apoptosis in human medulloblastoma cells, being valuable insight for cellular and molecular levels of JEV pathogenesis. PMID: 19463724
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亞細胞定位：[Capsid protein C]: Virion. Host nucleus. Host cytoplasm. Host cytoplasm, host perinuclear region.; [Peptide pr]: Secreted.; [Small envelope protein M]: Virion membrane; Multi-pass membrane protein. Host endoplasmic reticulum membrane; Multi-pass membrane protein.; [Envelope protein E]: Virion membrane; Multi-pass membrane protein. Host endoplasmic reticulum membrane; Multi-pass membrane protein. Host cell surface.; [Non-structural protein 1]: Secreted. Host endoplasmic reticulum membrane; Peripheral membrane protein; Lumenal side.; [Non-structural protein 2A]: Host endoplasmic reticulum membrane; Multi-pass membrane protein.; [Serine protease subunit NS2B]: Host endoplasmic reticulum membrane; Multi-pass membrane protein.; [Serine protease NS3]: Host endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side.; [Non-structural protein 4A]: Host endoplasmic reticulum membrane; Multi-pass membrane protein.; [Non-structural protein 4B]: Host endoplasmic reticulum membrane; Multi-pass membrane protein.; [RNA-directed RNA polymerase NS5]: Host endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Host nucleus.
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蛋白家族：Class I-like SAM-binding methyltransferase superfamily, mRNA cap 0-1 NS5-type methyltransferase family
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數據庫鏈接：
KEGG: vg:1489713
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