Recombinant Human herpesvirus 8 type P ORF57 protein (ORF57)

中文名稱：

Recombinant Human herpesvirus 8 type P ORF57 protein(ORF57)
貨號：

CSB-YP644149HKE
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

Recombinant Human herpesvirus 8 type P ORF57 protein(ORF57)
貨號：

CSB-EP644149HKE
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

Recombinant Human herpesvirus 8 type P ORF57 protein(ORF57)
貨號：

CSB-EP644149HKE-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Human herpesvirus 8 type P ORF57 protein(ORF57)
貨號：

CSB-BP644149HKE
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Human herpesvirus 8 type P ORF57 protein(ORF57)
貨號：

CSB-MP644149HKE
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

ORF57
Uniprot No.：

Q2HR75
別名：

ORF57mRNA export factor ICP27 homolog; EB2 protein homolog
種屬：

Human herpesvirus 8 type P (isolate GK18) (HHV-8) (Kaposi's sarcoma-associated herpesvirus)
蛋白長度：

full length protein
表達區域：

1-455
氨基酸序列

MVQAMIDMDI MKGILEDSVS SSEFDESRDD ETDAPTLEDE QLSEPAEPPA DERIRGTQSA QGIPPPLGRI PKKSQGRSQL RSEIQFCSPL SRPRSPSPVN RYGKKIKFGT AGQNTRPPPE KRPRRRPRDR LQYGRTTRGG QCRAAPKRAT RRPQVNCQRQ DDDVRQGVSD AVKKLRLPAS MIIDGESPRF DDSIIPRHHG ACFNVFIPAP PSHVPEVFTD RDITALIRAG GKDDELINKK ISAKKIDHLH RQMLSFVTSR HNQAYWVSCR RETAAAGGLQ TLGAFVEEQM TWAQTVVRHG GWFDEKDIDI ILDTAIFVCN AFVTRFRLLH LSCVFDKQSE LALIKQVAYL VAMGNRLVEA CNLLGEVKLN FRGGLLLAFV LTIPGMQSRR SISARGQELF RTLLEYYRPG DVMGLLNVIV MEHHSLCRNS ECAAATRAAM GSAKFNKGLF FYPLS
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Early protein that promotes the accumulation and nuclear export of viral intronless RNA transcripts by interacting with mRNAs and cellular export proteins. Probably acts as a viral splicing factor that regulates viral RNA splicing. Functions as a multifunctional regulator of the expression of viral lytic genes.
基因功能參考文獻：
1. data provide the first evidence that KSHV ORF57 plays a role in modulating PKR/eIF2alpha/SG axis and enhances virus production during virus lytic infection. PMID: 29084250
2. Herein, the authors extend their previous research and provide evidence that ORF57 of human herpesvirus-8 interacts with CHTOP and CIP29, in contrast to POLDIP3. PMID: 27189710
3. These data suggest that ORF57 is involved in modulating host gene expression in addition to KSHV gene expression during lytic reactivation. PMID: 25710169
4. Authors speculate that binding of particular hnRNPs to human herpesvirus 8 lytic transcripts is the prerequisite for ORF57 to enhance their expression. PMID: 25694606
5. The C-terminal domain of ORF57contributes to homodimerization of ORF57 to prevent proteasome-mediated protein degradation. PMID: 25568207
6. The eight genes identified to critically require ORF57 belong to both early and late lytic temporal classes, and seven are involved in DNA replication, virion assembly, or viral infectivity. PMID: 25410858
7. Results identified modulation of SRSF3 activity as the molecular mechanism by which ORF57 promotes RNA splicing. PMID: 25234929
8. ORF57 protein is a multifunctional protein involved in all stages of viral mRNA processing and is essential for virus replication. PMID: 23896747
9. These data indicate that PABPC1 is an important cellular target of human herpesvirus 8 ORF57 to directly upregulate polyadenylated nuclear RNA accumulation during viral lytic infection. PMID: 23077296
10. Although ORF57 promotes expression of a selection of KSHV viral intronless RNAs, it is not a bona fide export factor. PMID: 22993146
11. Human REF/Aly-ORF57 interaction is not essential for KSHV lytic replication but may contribute to target RNA stability independent of effects on RNA export, suggesting a novel role for REF/Aly in viral RNA metabolism. PMID: 22761374
12. findings show that ORF57 binds directly to the 5' end of PAN RNA in KSHV-infected cells. PMID: 21889182
13. results suggest the mutation of a carboxy-terminal RGG motif affects ORF57 RNA binding, nuclear trafficking, and multimerization PMID: 21593148
14. ORF57 interacts with RBM15 and OTT3 to promote expression of viral intronless genes. PMID: 21106733
15. Our data indicate that the expression of KSHV ORF57 is regulated by cellular RNA export factors and cofactors UAP56, URH49, RBM15, and OTT3 at the posttranscriptional level. PMID: 20828777
16. Kaposi sarcoma-associated herpesvirus ORF57 protein functions to enhance the nuclear stability of intronless viral transcripts by protecting them from a cellular RNA quality control pathway PMID: 20221435
17. caspase 7 cleavage of ORF57 may represent a cellular function against lytic KSHV infection PMID: 20159985
18. We propose that one molecular mechanism of Mta-mediated transactivation is a direct effect on transcription by direct and indirect promoter association. PMID: 17913801
19. The third RRE in the ORF57 promoter was identified and characterized in RTA mediated transactivation and induction of KSHV lytic replication. PMID: 19223488
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亞細胞定位：

Host cytoplasm. Host nucleus. Note=Shuttles between the nucleus and the cytoplasm.
蛋白家族：

HHV-1 ICP27 protein family
數據庫鏈接：

KEGG: vg:4961525