Recombinant Human adenovirus A serotype 12 Early E1A 29.5 kDa protein

中文名稱：

Recombinant Human adenovirus A serotype 12 Early E1A 29.5 kDa protein
貨號：

CSB-YP355995HIB
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

Recombinant Human adenovirus A serotype 12 Early E1A 29.5 kDa protein
貨號：

CSB-EP355995HIB
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

Recombinant Human adenovirus A serotype 12 Early E1A 29.5 kDa protein
貨號：

CSB-EP355995HIB-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Human adenovirus A serotype 12 Early E1A 29.5 kDa protein
貨號：

CSB-BP355995HIB
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Human adenovirus A serotype 12 Early E1A 29.5 kDa protein
貨號：

CSB-MP355995HIB
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

N/A
Uniprot No.：

P03259
別名：

Early E1A protein; Early E1A 29.5 kDa protein
種屬：

Human adenovirus A serotype 12 (HAdV-12) (Human adenovirus 12)
蛋白長度：

full length protein
表達區域：

1-266
氨基酸序列

MRTEMTPLVL SYQEADDILE HLVDNFFNEV PSDDDLYVPS LYELYDLDVE SAGEDNNEQA VNEFFPESLI LAASEGLFLP EPPVLSPVCE PIGGECMPQL HPEDMDLLCY EMGFPCSDSE DEQDENGMAH VSASAAAAAA DREREEFQLD HPELPGHNCK SCEHHRNSTG NTDLMCSLCY LRAYNMFIYS PVSDNEPEPN STLDGDERPS PPKLGSAVPE GVIKPVPQRV TGRRRCAVES ILDLIQEEER EQTVPVDLSV KRPRCN
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. E1A protein has both transforming and trans-activating activities. Induces the disassembly of the E2F1 transcription factor from RB1 by direct competition for the same binding site on RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes and of the E2 region of the adenoviral genome. Release of E2F1 leads to the ARF-mediated inhibition of MDM2 and causes TP53/p53 to accumulate because it is not targeted for degradation by MDM2-mediated ubiquitination anymore. This increase in TP53, in turn, would arrest the cell proliferation and direct its death but this effect is counteracted by the viral protein E1B-55K. Inactivation of the ability of RB1 to arrest the cell cycle is critical for cellular transformation, uncontrolled cellular growth and proliferation induced by viral infection. Interaction with RBX1 and CUL1 inhibits ubiquitination of the proteins targeted by SCF(FBXW7) ubiquitin ligase complex, and may be linked to unregulated host cell proliferation. The tumorigenesis-restraining activity of E1A may be related to the disruption of the host CtBP-CtIP complex through the CtBP binding motif. Interaction with host TMEM173/STING impairs the ability of TMEM173/STING to sense cytosolic DNA and promote the production of type I interferon (IFN-alpha and IFN-beta). Promotes the sumoylation of host ZBED1/hDREF with SUMO1.
基因功能參考文獻：
1. Human adenovirus E1A and E1B early oncogene proteins expressed in primary progenitor cells transform them to exhibit features of tumor cells. PMID: 27795433
2. Adenovirus E1A oncoprotein is sufficient to induce Smad7 expression, an inhibitor of TGF-beta signalling. PMID: 19885573
亞細胞定位：

Host nucleus.
蛋白家族：

Adenoviridae E1A protein family
數據庫鏈接：

KEGG: vg:1460853