Recombinant Human Vascular cell adhesion protein 1 (VCAM1), partial

中文名稱：

人VCAM1重組蛋白
貨號：

CSB-YP025809HU
說明書：

MSDS Datasheet
規格：

￥1500
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

VCAM1
Uniprot No.：

P19320
別名：

CD106; CD106 Antigen; INCAM 100; INCAM-100; L1CAM; MGC99561; V-CAM 1; Vascular Cell Adhesion Molecule 1; Vascular cell adhesion protein 1; VCAM 1; VCAM-1; VCAM1; VCAM1_HUMAN
種屬：

Homo sapiens (Human)
蛋白長度：

Extracellular Domain
來源：

Yeast
分子量：

76.2kDa
表達區域：

25-698aa
氨基酸序列

FKIETTPESRYLAQIGDSVSLTCSTTGCESPFFSWRTQIDSPLNGKVTNEGTTSTLTMNPVSFGNEHSYLCTATCESRKLEKGIQVEIYSFPKDPEIHLSGPLEAGKPITVKCSVADVYPFDRLEIDLLKGDHLMKSQEFLEDADRKSLETKSLEVTFTPVIEDIGKVLVCRAKLHIDEMDSVPTVRQAVKELQVYISPKNTVISVNPSTKLQEGGSVTMTCSSEGLPAPEIFWSKKLDNGNLQHLSGNATLTLIAMRMEDSGIYVCEGVNLIGKNRKEVELIVQEKPFTVEISPGPRIAAQIGDSVMLTCSVMGCESPSFSWRTQIDSPLSGKVRSEGTNSTLTLSPVSFENEHSYLCTVTCGHKKLEKGIQVELYSFPRDPEIEMSGGLVNGSSVTVSCKVPSVYPLDRLEIELLKGETILENIEFLEDTDMKSLENKSLEMTFIPTIEDTGKALVCQAKLHIDDMEFEPKQRQSTQTLYVNVAPRDTTVLVSPSSILEEGSSVNMTCLSQGFPAPKILWSRQLPNGELQPLSENATLTLISTKMEDSGVYLCEGINQAGRSRKEVELIIQVTPKDIKLTAFPSESVKEGDTVIISCTCGNVPETWIILKKKAETGDTVLKSIDGAYTIRKAQLKDAGVYECESKNKVGSQLRSLTLDVQGRENNKDYFSPE
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產品描述：

A truncated version of Vascular cell adhesion protein 1 (VCAM1) encoding the extracellular domain and carrying a 6xHis-tag at the N-terminus was produced in the yeast. The resulting protein is a partial-length VCAM1 protein. It contains aa of 25-698 of the human VCAM1 protein. It was purified by SDS-PAGE and reached up to 90% in purity. On the reducing SDS-PAEG gel, this VCAM1 protein migrated on the molecular weight band of about 66-116 kDa. In addition to producing specific antibodies, the recombinant VCAM1 protein could also be used in the studies of VACM-related immunology or signal transduction.

VCAM-1, or called CD106, is a glycoprotein primarily expressed in endothelial cells. Pro-inflammatory cytokines, ROS, high glucose concentration, oxidized low-density lipoprotein, shear stress, and toll-like receptor agonists, can induce the expression of VCAM-1. VCAM-1 participates in the modulation of inflammation-associated vascular adhesion and the transendothelial migration of leukocytes. Recent studies show the close link of VCAM-1 to the progression of various immunological disorders, such as asthma, rheumatoid arthritis, transplant rejection, and cancer.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
基因功能參考文獻：
1. High VCAM1 expression is associated with Cardiovascular Disease. PMID: 28496046
2. not a specific screening marker for pulmonary arterial hypertension in systemic sclerosis PMID: 29687288
3. We observed an increase of VCAM-1 urine levels in diabetic nephropathy-basal patients compared to diabetic controls and an increase of urinary neprilysin in DN-treated patients with persistent albuminuria PMID: 29854824
4. Urinary angiostatin and VCAM-1 are predictive of specific histological changes in concurrent lupus nephritis renal biopsies. PMID: 29076253
5. In summary, it could be concluded that the expression of gene VCAM-1 was down regulated by taurocholic acid through miR-590-3p and that gene VCAM-1 could participate in the regulation of cell growth, cell proliferation and cell apoptosis PMID: 28851649
6. VCAM-1 expression is upregulated by CD34+/CD133+-stem cells derived from septic patients PMID: 29601599
7. Circulating soluble vascular cell adhesion molecule (VCAM-1) level was a useful diagnostic parameter for understanding the difference between coronary artery disease (CAD) and cardiac syndrome X (CSX). PMID: 28675064
8. findings indicate a novel role for JAK2-V617F in activation of beta1 integrins and enhanced adhesion of granulocytes and 32D myeloid progenitors to VCAM1-coated surfaces PMID: 28096537
9. High VCAM1 expression is associated with Cardiovascular Disease Risk. PMID: 28202840
10. VCAM-1 levels are elevated in serum of cystic fibrosis patients. PMID: 28646244
11. VCAM-1 levels are able to accurately predict significant fibrosis in non-alcoholic fatty liver disease patients PMID: 28461687
12. These results revealed the effect of CD106 and NF-kappaB in bone marrow failure of Acquired aplastic anemia. PMID: 28764810
13. Overexpression of LINC00341 suppresses the expression of vascular cell adhesion molecule 1 (VCAM1) and the adhesion of monocytes induced by atheroprone flow and tumor necrosis factor-alpha. PMID: 28500253
14. Increased expression of PECAM-1, ICAM-3, and VCAM-1 in colonic biopsies from patients with inflammatory bowel disease (IBD) in clinical remission is associated with subsequent flares; this suggests that increases in the expression of these proteins may be early events that lead to flares in patients with IBD PMID: 27552332
15. Association with the efficiency of hematopoietic stem cells (HSC) mobilization to PB were found in the SNPs of the DGKB gene involved in cell transport and SDF-1-induced migration ability and of the VCAM1 gene which is essential for HSC homing, suggesting that SNPs involved in cell migration ability might be partly involved in HSC PMID: 27839966
16. sVCAM-1 reflects xerostomia in primary Sjogren's syndrome. sICAM-1 and sE-selectin may be additional parameters of secondary Sjogren's syndrome activity. PMID: 29068581
17. Augmented expression of endothelial adhesion molecules ICAM1/VCAM1 is involved in the pathophysiology of patients with antiphospholipid syndrome. PMID: 29096830
18. VCAM-1 knockdown reduced TNFalpha-induced HUVEC migration and tube formation. PMID: 28209985
19. High levels of serum ICAM-1 was associated with the development of multiple organ failure . High levels of VCAM-1 was associated with both multiple organ failure and in-hospital mortality. PMID: 27701021
20. VCAM1 protein expression increased in cartilage tissue from spinal tuberculosis patients. PMID: 28829887
21. This study provided direct evidence that increasing extracellular Ca(2+) enhanced TNF-alpha-induced VCAM-1 activation and monocytes adhesion. Moreover, we identified a novel TRPC1/ERK1/2/NFkappaB signaling pathway mediating VCAM-1 activation and monocyte adhesion in this pathological process. PMID: 28583863
22. Findings suggest that sVCAM-1 is related to affective temperaments, and it is a trait marker for liability to mood disorders. This relationship between alterations in cellular adhesion and affective temperament may be important for vulnerability to affective disorders. PMID: 27693464
23. patients with newly-diagnosed MM have increased serum levels of VCAM-1 and ICAM-1 that correlate with advanced disease features. VCAM-1 is also independently correlated with survival, suggesting an important role in the biology of the disease and supporting its targeting with novel anti-myeloma drugs. PMID: 27232930
24. VCAM-1 was statistically elevated in major depressive disorder. PMID: 27598970
25. Data show that podoplanin (PDPN), CD106 (VCAM-1) and CD248 protein were increased in diseased compared to healthy tendon cells. PMID: 28122639
26. Peptide nanoparticles targeting VCAM-1 are suggested as a theranostic carrier for drug delivery to inflamed endothelium. PMID: 27105996
27. IRF-5 targeted the expression of vascular cell adhesion molecule 1 (VCAM-1) at the transcriptional level by binding to its promoter. PMID: 28818665
28. In patients with optic neuritis that developed into multiple sclerosis, there was a decrease in serum VCAM-1. PMID: 27806869
29. There was a negative correlation of miR-126 with the PCI-induced markers of inflammation such as hs-CRP and VCAM-1 PMID: 27900989
30. this study shows that quercetin reduces the expression of VCAM-1 in oxidized LDL cholesterol-induced endothelial cells PMID: 27585526
31. Gastrin-releasing peptide (GRP) increased the expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) by activating nuclear factor-kappaB (NF-kappaB) in endothelial cells. PMID: 28093230
32. Mesothelium VCAM-1 expression is negatively associated with progression-free and overall survival in EOC. This is especially compelling in light of previous data suggesting that persistent VCAM-1 expression during treatment is an indicator of platinum resistance. PMID: 28263384
33. The results of this study suggest that VCAM-1 gene variants may be related to obesity and inflammatory markers in the Korean population PMID: 27853845
34. Panax notoginseng saponins and Ginsenoside protect aorta form atherosclerosis via Rb1 via activation of Nrf2 and suppression of p38 - VCAM-1 signal pathway. PMID: 27620662
35. Our results suggest that an impairment in adhesion exists in vitiligo skin, which is supported by the diminished immunohistochemical expression of laminin, beta1 integrin, ICAM-1 and VCAM-1. PMID: 28214208
36. Th1-induced CD106 expression mediates leukocytes adhesion on synovial fibroblasts from juvenile idiopathic arthritis patients via TNF-alpha. PMID: 27123929
37. Although the expression of VCAM-1 was higher in adult T-cell leukemia/lymphoma patients individuals, there was no significant difference between the studied groups. PMID: 28110427
38. Activin A and preeclamptic serum upregulate ET-1, ICAM-1, and VCAM-1 in human umbilical vein endothelial cells, consistent with activin A contributing to the pathophysiology of preeclampsia. PMID: 27155341
39. Statistically significant associations were identified between the chronic periodontal patients and the controls in the gene polymorphisms of rs5498 ICAM-1 (P=0.007) and rs1041163 VCAM-1 (P=0.029). The distribution of rs5498 (P=0.029) and rs1041163 (P=0.049) differed significantly across the mild, moderate, and severe groups of periodontitis. PMID: 27391418
40. VCAM-1 had a synergistic effect with coronary artery disease on the BDNF PMID: 28131673
41. findings suggest that the increased levels of IL-17,Hcy and sVCAM contributes contributes to the vascular inflammation and activation presented by sickle cell anemia patients PMID: 26334689
42. VCAM-1 plays an important role in the development and generation of gestational hypertension. PMID: 27706617
43. gLDL stimulate the expression of cellular VCAM-1, the secretion of soluble VCAM-1, and the adhesion of monocytes through mechanisms involving p38 MAP kinase and NF-kB signalling pathways activated by RAGE, ERS and oxidative stress, thus contributing to diabetic atherosclerosis. PMID: 27206739
44. High VCAM1 expression is associated with non-insulin resistant women with polycystic ovary syndrome. PMID: 27245110
45. Denbinobin inhibited IL-1beta-induced ICAM-1/VCAM-1 expression and monocyte adhesion to osteoarthritis fibroblast-like synoviocytes via mirn146-a PMID: 25052989
46. Theobroma cacao extract prohibits the increase in IL-6 and sVCAM-1 in endothelial cells induced by plasma from preeclamptic patients. PMID: 26955771
47. BD patients have a lower expression of ICAM-1, VCAM-1, and E-selectin in their tumor tissues. BD-HDL facilitates the adhesion of tumor cells to vascular endothelium by upregulating the expression of ICAM-1 and VCAM-1, thereby promoting the initial progression of breast cancer metastasis PMID: 26872904
48. Clopidogrel diminishes TNFalpha-stimulated VCAM-1 expression at least in part via HO-1 induction and CaMKKbeta/AMPK/Nrf2 pathway in endothelial cells. PMID: 26824050
49. Results show that VCAM1 expression is regulated by TSPO to activate endothelial cells. PMID: 26608360
50. Human placental, chorionic villi-derived mesenchymal stem cells expressing VCAM-1 promote angiogenesis in a mouse ischemia model. PMID: 27044487
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亞細胞定位：

Membrane; Single-pass type I membrane protein.
組織特異性：

Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue.
數據庫鏈接：

HGNC: 12663

OMIM: 192225

KEGG: hsa:7412

STRING: 9606.ENSP00000294728

UniGene: Hs.109225