E. coli biotin ligase
(BirA) is highly specific in covalently attaching biotin to the 15
amino
acid AviTag peptide. This recombinant protein was biotinylated in
vivo
by AviTag-BirA technology, which method is BriA catalyzes amide
linkage
between the biotin and the specific lysine of the AviTag.
The tag type will
be
determined during production process. If you have specified tag
type, please tell us and we will develop the specified tag
preferentially.
產品提供形式:
Lyophilized
powder
Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
Note: We will
preferentially ship the format that we have in stock, however,
if you have any special requirement for the format, please
remark your requirement when placing the order, we will prepare
according to your demand.
復溶:
We recommend that this vial be briefly centrifuged
prior
to opening to bring the contents to the bottom. Please reconstitute
protein in deionized sterile water to a concentration of 0.1-1.0
mg/mL.We recommend to add 5-50% of glycerol (final concentration)
and
aliquot for long-term storage at -20℃/-80℃. Our default final
concentration of glycerol is 50%. Customers could use it as
reference.
儲存條件:
Store at -20°C/-80°C upon receipt, aliquoting is
necessary for
mutiple use. Avoid repeated freeze-thaw cycles.
保質期:
The shelf life is related to many factors, storage
state,
buffer ingredients, storage temperature and the stability of the
protein
itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C.
The
shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期:
Delivery time may
differ from different purchasing way or location, please kindly
consult your local distributors for specific delivery time.
Note: All of our
proteins are default shipped with normal blue ice packs, if you
request to ship with dry ice, please communicate with us in
advance
and extra fees will be charged.
注意事項:
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Acts as transcription regulator that binds target promoter DNA and bends the DNA. Binds to the sequences 5'-AACAAT-'3 or 5'-AACAAAG-3'. Modulates transcriptional regulation via WNT3A. Inhibits Wnt signaling. Promotes degradation of activated CTNNB1. Plays a key role in the regulation of embryonic development. Required for normal development of the definitive gut endoderm. Required for normal looping of the embryonic heart tube. Plays an important role in embryonic and postnatal vascular development, including development of arteries. Plays an important role in postnatal angiogenesis, where it is functionally redundant with SOX18. Required for the generation and maintenance of fetal hematopoietic stem cells, and for fetal hematopoiesis. Probable transcriptional activator in the premeiotic germ cells.
基因功能參考文獻:
Case-control analyses reveal significant overrepresentation of rare variants in ATP13A3, AQP1 and SOX17, and provide independent validation of a critical role for GDF2 in heritable pulmonary arterial hypertension. PMID: 29650961
These data strongly implicate SOX17 as a new risk gene contributing to Pulmonary arterial hypertension-congenital heart disease. PMID: 30029678
This study showed that 1 of 4 SNPs located near the SOX17 gene, rs1072737, was statistically associated with intracranial aneurysm (IA) formation in a Korean population. The MAF of this variant (minor allele, C) showed a highly ethnic difference between Korean and European populations. Two SNPs rs10958409 and rs9298506 showed statistically significant associations with increased IA risk in the current meta-analysis. PMID: 29191544
The combination of methylated SOX17 with cytology better predicted neoplastic grade than cytology alone PMID: 28148542
SOX17 acts as a tumor suppressor in cholangiocarcinoma and its genetic, molecular and/or pharmacological restoration may represent a new promising therapeutic strategy. PMID: 28237397
Our results suggest that decreasing SOX17 levels may promote EC development and progression, and that by downregulating MAML3 expression and Wnt signaling, SOX17 acts as a tumor suppressor that may improve outcome in patients with EC. PMID: 27738313
SOX2 repression in TCam-2 cells can be abrogated by recruitment of the constitutively expressed H3K27 demethylase UTX to the SOX2 promoter through retinoid signaling, leading to expression of neuronal and other lineage genes. SOX17 has been shown to initiate human PGC specification, with its target PRDM1 suppressing mesendodermal genes PMID: 27132888
Dedifferentiation of fibroblasts to CD34(+) progenitor cells gives rise to endothelial cells and erythroblasts in a SOX17-dependent manner. PMID: 28381471
Study extracted and analyzed the experimentally validated 3D models of SOX17-HMG domain and beta-catenin; the molecular level disturbance in the two essential human proteins upon M76A and G103R mutation of SOX17, which further hampers the cell signaling phenomena for the human cytological developments beginning from gastrulation and endoderm formation. PMID: 28132771
SOX17 promoter is highly methylated in primary tumors and in corresponding plasma samples both in operable and advanced non-small cell lung cancer. PMID: 26741346
The definitive endoderm and foregut endoderm differentiation capabilities of Wnt pathway-modulated cells were determined based on the expression levels of the endodermal transcription factors SOX17 and FOXA2 and those of the transcription activator GATA4 and the alpha-fetoprotein (AFP) gene, respectively. PMID: 26861571
Knockdown of OCT4 during differentiation inhibits mesendoderm formation and removal of the H3K27me3 mark from the SOX17 promoter, suggesting that OCT4 acts to induce removal of the Polycomb2 complex. PMID: 26411902
high expression of the Sox17 associated pathway in medium and small arteries indicates that Brain arteriovenous malformation vessels are intrinsically active PMID: 26463399
SOX17 demethylation induced miR-371-5p expression and consequently suppressed its direct target SOX2. A novel mechanism of the SOX17/miR-371-5p/SOX2 axis is involved in the regulation of EMT, stemness and metastasis. PMID: 25868860
Decreased expression of SOX17 is associated with tumor progression in breast cancer. PMID: 25971583
In this study, oligodendroglioma patients with 1p/19q LOH and Sox17 protein expression had a better prognosis. PMID: 25674225
SOX17 might play a role in the development of Ovarian Cancer, and may be a therapeutic target in OC. PMID: 25953442
Decreased Sox17 expression is correlated with melanoma progression, an unfavorable survival of melanoma patients and is an independent molecular prognostic factor for melanoma. PMID: 25310020
Possible association of SOX-17 and RBBP8 with brain arteriovenous malformations, genes involved in cell cycle progression, deserves further investigation PMID: 25053769
promoter methylation may play an important role in breast cancer progression and could be used as a prognostic biomarker to identify patients at risk of developing metastasis or recurrence after mastectomy PMID: 25789956
Human intracranial aneurysm samples showed reduced Sox17 expression and impaired endothelial integrity. PMID: 25596186
Low Sox17 expression is associated with hepatocellular carcinoma. PMID: 25106407
SOX17 is the key regulator of human primordial germ cells-like cells specification, whereas BLIMP1 represses endodermal and other somatic genes during hPGCLC specification. PMID: 25543152
Hypermethylation of SOX17 promoter may be one of the early events in the development of myelodysplatic syndrome and predicts poor prognosis. PMID: 25291942
Low SOX17 expression is associated with esophageal cancer progression. PMID: 24407731
Experimental confirmation of miRNA-mRNA interactions established a critical role of miR-200a in regulating both EMT and definitive endoderm formation, through direct repression of ZEB2 and SOX17, during early stage differentiation. PMID: 23813959
In multiple sclerosis tissue, Sox17 was primarily detected in actively demyelinating lesions and periplaque white matter. PMID: 23918253
Immunofluorescence analysis of human pancreatic tissue arrays revealed the presence of tuft cells in metaplasia and early-stage tumors, along with SOX17 expression, consistent with a biliary phenotype. PMID: 23999170
Overall survival of patients with gastric cancer was found to be significantly associated with SOX17 promoter methylation. PMID: 23403728
after electrostatic interactions attract Sox17 to DNA, Asn73, Ser99, and Trp106 form hydrogen bonds with DNA, Arg70, Lys80, Arg83, His94, and Asn95 on Sox17 undergo conformational changes and form hydrogen bonds with DNA PMID: 23061670
recombinant Sox17 mediates modulation of the Wnt pathway through changes in beta-catenin, SFRP1 and Wnt/Frizzled expression. PMID: 23474492
SOX17 promoter is highly methylated in primary breast tumors, in CTCs isolated from patients with breast cancer, and in corresponding cfDNA samples PMID: 23136251
SOX17 plays a key role in priming hemogenic potential in endothelial cells, thereby regulating hematopoietic development from stem cells. PMID: 23169777
findings establish Sox17 as a key regulator of tumor angiogenesis and tumor progression. PMID: 23241958
SOX17 was frequently methylated in human PTC. Loss of SOX17 expression was induced by promoter region hypermethylation. SOX17 inhibited thyroid cancer proliferation. Methylation of SOX17 activated the Wnt signaling pathway in human thyroid cancer. PMID: 23044318
SOX17 promoter region is frequently methylated in esophageal cancer and in a progression tendency during esophageal carcinogenesis. PMID: 22921431
provided further evidence to support the previously reported association of intracranial aneurysm with single nucleotide polymorphism in SOX17 PMID: 22961961
New sequence variations in SOX17 were identified but all correspond to nonpathogenic variants, suggesting that SOX17 is not involved in UHL phenotype PMID: 22348788
silencing of Sox17 occurs frequently in early gastric cancer PMID: 22161215
Downstream targets of Sox17 define signaling pathways and molecular mechanisms in oligodendrocyte progenitor cells that are regulated by Sox17 during cell cycle exit and differentiation in oligodendrocyte development. PMID: 21957254
A stage-specific transduction of SOX17 in the primitive endoderm or mesendoderm promotes directive extraembryonic endoderm or definitive endoderm differentiation by SOX17 transduction, respectively. PMID: 21760905
Sox17 might be a key transcription factor controlling CD133 expression, and that it might also play a role in the control of gastric tumor progression. PMID: 21457403
Data show that down-regulated by Sox17 expressing HepG2 cells is a set of genes that are expressed in the developing liver, suggesting that one function of Sox17 is the repression of liver gene expression. PMID: 21305474
Sox17 prominently contributes to gastric cancer progression through regulating proliferation and cell cycle, indicating a novel diagnosis and prognosis biomarker as well as a potential therapeutic target in gastric cancer PMID: 21514720
SOX17 negatively regulates canonical WNT/beta-catenin signaling pathway and inhibits human HCC cells growth PMID: 20716954
These data indicate a role of SOX17 in human kidney and urinary tract development and implicate the SOX17-p.Y259N mutation as a causative factor in congenital anomalies of the kidney and the urinary tract . PMID: 20960469
Sox17 may be a valuable biomarker for the study of breast cancer carcinogenesis and progression. PMID: 19301122
SOX17 silencing due to promoter hypermethylation is an early event during tumorigenesis and may contribute to aberrant activation of Wnt signaling in colorectal cancer. PMID: 18413743
顯示更多
收起更多
相關疾病:
Vesicoureteral reflux 3 (VUR3)
亞細胞定位:
Nucleus.
組織特異性:
Expressed in adult heart, lung, spleen, testis, ovary, placenta, fetal lung, and kidney. In normal gastrointestinal tract, it is preferentially expressed in esophagus, stomach and small intestine than in colon and rectum.